Determine the Essential Micronutrients Intake by Vibrio cholerae in Association with Algae in Different Waters

Abstract

Phosphate, nitrite, nitrate are the micronutrients for Vibrio cholerae and algal growth. Current study intended to determine the utilization of these micronutrients by V. cholerae in association with algae in four different water (Distilled water, River water, Aritificial sea water and BOD dilution water). Amount of these micronutrients were estimated from stock water, control water and algal water by developing standard curve method. Among these four stock water, orthophosphate was the highest in amount (10.44µg/L) in BOD dilution stock water and significant positive changes (0.06 µg/L) from stock found in the algal water in River water microcosm. In stock sample , the highest amount of nitrite was found in both River and BOD dilution water (6.486µg/L) and the most significant increase ( 388.014µg/L) from stock was found in the control water of BOD dilution water. River stock water contained the maximum nitrate (1865.204µg/L) among four water types and the highest increase was found in the control water of River. In brief, the amount of phosphate, nitrite and nitrate varied with the types of water. Most cases amount of nitrite- nitrate increased by the decay of V. cholerae which used by algae for their growth and overloaded algae did not use phosphate from the surrounding water.