Determination Trace Levels of Vitamin C and Folic Acid in Urine Sample by Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction Method Coupled HPLC-UV

Sheikh-Akbari, Akbar; Chamkouri, Narges; Zadabdollah, Atefeh

doi:10.13005/ojc/320623

Cited by 7 publications

(3 citation statements)

References 15 publications

(16 reference statements)

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“…The colorimetric change during XTT assay, expressed as OD, was measured using a microtiter plate reader at A505 [40]. Higher OD values indicate an increased number of surviving candida in the biofilm.…”

Section: Quantitative Analysis -Xtt Reduction Assaymentioning

confidence: 99%

Chitosan Nanogel Design on Gymnema sylvestre Essential Oils to Inhibit Growth of Candida albicans Biofilm and Investigation of Gene Expression ALS1, ALS3

Akbari

Bayat

Roudbarmohammadi

et al. 2019

Period. Polytech. Chem. Eng.

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Chitosan (CS) is a polycation with a pka of about 6.3 with a charge density dependent on pH and the %DA-value which can interact with polyanions to form complex and gels. Nanoparticles (CNPs) in addition can increase the antifungal potential of bioactive compounds like essential oils by increasing cellular interactions between them and the fungal as a result of the very small size that enhances cellular uptake. In this study, was set to investigate the encapsulation of the Gymnema sylvestre essential oils (G.EOs) using Chitosan and Myrestic acid made Nanogel in order to enhance its antifungal activity and stability to the oil against C. albicans strain (ATCC 10231). To procedure this, the self-assembled process of Chitosan and Myrestic acid Nanogel (CS-MA) through the 1- ethyl - 3- (3 dimethyl aminoprophyl) carbodiimide (EDC) was designed. Its physicochemical properties were determined by Fourier Transforms Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and microscopic methods by Atomic force microscopy (AFM), Transmission Electron Microscopy (TEM), and Scanning Electron Microscopy (SEM). Minimum inhibitory concentration (MIC) at 18.7 to 37.5 µg/ml and 2.3 to 4.6 µg/ml and minimal fungicidal concentration (MFC) at 75 µg/ml and 5.38 µg/ml using by broth micro dilution (BMD) method for G. sylvestre oils (G.EOs) and oil-loaded Nanogels (G.OLNPs) were measurement. The susceptibility of C. albicans biofilm to fractions was examined by 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[8phenylamino) carbonyl] - 2H - tetrazolium hydroxide (XTT) quantitatively.The concentration of G.OLNPs required to inhibit 50 % biofilm formation was 4.68 µg/ml, while that to remove 90 % biofilm growth was 18.07 µg/ml. In addition, it was observed that cell uptake of G.OLNPs was much higher compared with free G.EOs. Reverse transcription polymerase chain reaction (RT-PCR) analysis was performed to determine the effect of sub-MIC concentrations of G.EOs and G.OLNPs on expression of the biofilm–related gene ALS1 / ALS3, and indicated the G.OLNPs down-regulated the expression of hypha-specific gene ALS3. Furthermore, the data strongly suggested that G.OLNPs more effective suppressed C. albicans planktonic cells and reduction biofilm biomass.

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Section: Quantitative Analysis -Xtt Reduction Assaymentioning

confidence: 99%

Chitosan Nanogel Design on Gymnema sylvestre Essential Oils to Inhibit Growth of Candida albicans Biofilm and Investigation of Gene Expression ALS1, ALS3

Akbari

Bayat

Roudbarmohammadi

et al. 2019

Period. Polytech. Chem. Eng.

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“…On the other hand, vitamin C (ascorbic acid) has several different functions in humans and other mammals. In addition to its well-known role as an antioxidant, the vitamin serves as a co-factor in several important enzymatic reactions, including synthesis of catecholamines, carnitine, cholesterol, amino acids, and certain peptide hormones (Akbari et al 2016;Animoku et al 2018). Of course, its bestknown function is to facilitate the hydroxylation of proline and lysine residues in collagen, allowing proper intracellular folding of pro-collagen for export and deposition as mature collagen (Padayatty and Levine 2016).…”

Section: Introductionmentioning

confidence: 99%

Vitamin C Attenuate Neurological Changes in the Medial Prefrontal Cortex of Juvenile Mice Exposed to Diclofenac Sodium

Adekomi¹,

Adekeye²,

Adewale³

et al. 2022

Nig J Neurosci

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Diclofenac sodium is one of the commonly used therapeutic non-steroidal anti-inflammatory drugs; notwithstanding, diverse adverse effects are clearly described. In humans, vitamin C is an essential nutrient that is ubiquitously a water-soluble electron donor with biological characteristics. To a greater extent, it has been widely recognized not only as an antioxidant but also as a specific co-factor in patho-enzymatic processes and reactions. This study investigated the effect of vitamin C on the medial prefrontal cortex (mPFC) of juvenile mice exposed to Diclofenac sodium. Thirty juvenile mice were randomly assigned into 5 experimental groups; control, saline-treated, vitamin C treated, Diclofenac sodium treated, and vitamin C + Diclofenac sodium. Histochemical, immunohistochemical, stereologi-cal, and quantitative neurochemical studies were respectively, employed to assess the effect of vitamin C on Diclofenac sodium-associated neurological damage. Results showed that the histoarchitectural profile of the mPFC was well preserved in the control, saline, vitamin C, and vitamin C + Diclofenac sodium treated groups compared with the Diclofenac sodium treated group. Exposure to Diclofenac sodium during elicited significant glutamate level reduction in the mPFC. Furthermore, co-administration of vitamin C + Diclofenac sodium significantly decreased (p<0.05) glutamate level compared with the Diclofenac sodium-treated group. It could be concluded from this study that vitamin C conferred neuroprotective effect on the mPFC of the juvenile mice exposed to Diclofenac sodium.

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“…In this way, all microorganisms are killed without causing pathogenesis. In plasma sterilization, in addition to killing bacteria, dead bacteria are removed from the surface of the material due to a process similar to that of plasma engraving [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

The eliminatory effects of cold argon plasma jet on aflatoxin B1 produced by different isolates of Aspergillus section nigri

Hassanpour

Bayat

Nosrati

et al. 2019

BCC

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Aflatoxin and ochratoxin are among the most resistant and severe toxins produced by Aspergillus spp. The aim of this study was an evaluation of eliminatory effects of cold argon plasma jet on aflatoxin B1. Sampling of wheat, corn, oatmeal, flour and rice products from northern cities of Iran was carried out. The fungal species were cultured on CHAPK medium and next to sabouraud dextrose broth + malt extract (SB + ME) and also SB + yeast extract (SB + YE) media to obtain aflatoxin B1. The ELISA test was conducted to measure the aflatoxin level. The mean initial concentration of the aflatoxin from charts analysis in the SB +ME medium was 16.106 μg / kg (10.4, 8.32 and 7.55 μg / kg at 30, 60 and 360 sec, respectively), and in the SB +YE medium from 23.699 μg / kg (12.82, 9.93 and 9.54 μg / kg, respectively), at the same time. Furthermore, the mean concentrations of aflatoxin B1 by tables analysis in the SB+ ME medium changed from 20.02 μg / kg to 6.87, 10.46 and 7.55 μg / kg, at 30, 60 and 360 sec, respectively, and in the medium SB +YE from 28.85 μg / kg reached 6.56.6, 8.43 and 7.02 μg / kg, respectively, at the same time. In this study, the change or decrease in the concentration of aflatoxin B1, from Lin/Log and Log/Lin analyses of charts was significant (p<0.05) at 60 s and 360 s of application of the cold argon plasma jet in both media. The cold plasma as a new technology in various fields of food and agriculture can provide effective and suitable solutions in order to promote the goals of food industry, especially in the areas of mycotoxin elimination.

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Determination Trace Levels of Vitamin C and Folic Acid in Urine Sample by Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction Method Coupled HPLC-UV

Cited by 7 publications

References 15 publications

Chitosan Nanogel Design on Gymnema sylvestre Essential Oils to Inhibit Growth of Candida albicans Biofilm and Investigation of Gene Expression ALS1, ALS3

Chitosan Nanogel Design on Gymnema sylvestre Essential Oils to Inhibit Growth of Candida albicans Biofilm and Investigation of Gene Expression ALS1, ALS3

Vitamin C Attenuate Neurological Changes in the Medial Prefrontal Cortex of Juvenile Mice Exposed to Diclofenac Sodium

The eliminatory effects of cold argon plasma jet on aflatoxin B1 produced by different isolates of Aspergillus section nigri

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