Determination the levels of total bilirubin, total protein and liver histopathological changes in hepatotoxic rats and the role of Beta vulgaris roots ethanolic extract

Abstract

The present study was done to investigate the therapeutic efficiency of Beta vulgaris roots ethanolic extract versus N-acetylcysteine (NAC) in overcoming experimentally induced hapatotoxicity in rats by given double maximum therapeutic oral dose of Acetaminophen (N-acetyl-para-aminophenol (APAP). The experiment was performed by two parts: First part was involved the extraction of Beta vulgaris roots by using 90% ethanolic solvent and identification the main phytochemical constituents of Beta vulgaris roots ethanolic extract by GC-MS spectrometry analysis , while the second part of experiment was involved the determination and understand the therapeutic effect of Beta vulgaris roots ethanolic extract on hepatotoxicity induced by Acetaminophen which administered more than the recommended dose in rats. Three treated groups of male albino rats (12 rats each) and the dosing regimen was performed as follows: Each group was dosed (APAP) twice daily of (100 mg/kg) for 14 days as follows dosing regimen: Positive control group: Rats were daily administered Acetaminophen at dose (100 mg/kg) orally twice a day as (50 mg/kg) in the morning and (50 mg/kg) at the evening for 14 days followed by two weeks recovery.