Determination of δ13C values of sedimentary straight chain and cyclic alcohols by gas chromatography/isotope ratio mass spectrometry

Jones, Dorian P; Carter, J. F.; Eglinton, G.; Jumeau, E. J.; Fenwick, C. S.

doi:10.1002/bms.1200201009

Cited by 98 publications

(42 citation statements)

References 5 publications

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“…In this regard, future combinations of both technologies for two-tiered complementary analysis could provide plant physiologists with a sophisticated toolbox for initial screening of several distinct phenotypes, thereby identifying the most promising ones for more comprehensive analysis. The highprecision GC-C-IRMS analysis, demonstrated here for amino acids, might be easily widened to fatty acids (Meier-Augenstein, 2002;Richter et al, 2010;Panetta and Jahren, 2011), sterols (Jones et al, 1991), monosaccharides (Docherty et al, 2001;Derrien et al, 2003), and lignin (Goñi and Eglinton, 1996), which are all accessible via such an instrumentation. This promises to extend the approach to other pathways of primary metabolism (e.g.…”

Section: Resultsmentioning

confidence: 99%

Novel Approach for High-Throughput Metabolic Screening of Whole Plants by Stable Isotopes

Dersch

Beckers²,

Rasch³

et al. 2016

Plant Physiol.

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Here, we demonstrate whole-plant metabolic profiling by stable isotope labeling and combustion isotope-ratio mass spectrometry for precise quantification of assimilation, translocation, and molecular reallocation of 13 CO 2 and 15 NH 4 NO 3 . The technology was applied to rice (Oryza sativa) plants at different growth stages. For adult plants, 13 CO 2 labeling revealed enhanced carbon assimilation of the flag leaf from flowering to late grain-filling stage, linked to efficient translocation into the panicle. Simultaneous 13 CO 2 and 15 NH 4 NO 3 labeling with hydroponically grown seedlings was used to quantify the relative distribution of carbon and nitrogen. Two hours after labeling, assimilated carbon was mainly retained in the shoot (69%), whereas 7% entered the root and 24% was respired. Nitrogen, taken up via the root, was largely translocated into the shoot (85%). Salt-stressed seedlings showed decreased uptake and translocation of nitrogen (69%), whereas carbon metabolism was unaffected. Coupled to a gas chromatograph, labeling analysis provided enrichment of proteinogenic amino acids. This revealed significant protein synthesis in the panicle of adult plants, whereas protein biosynthesis in adult leaves was 8-fold lower than that in seedling shoots. Generally, amino acid enrichment was similar among biosynthetic families and allowed us to infer labeling dynamics of their precursors. On this basis, early and strong 13 C enrichment of Embden-Meyerhof-Parnas pathway and pentose phosphate pathway intermediates indicated high activity of these routes. Applied to mode-of-action analysis of herbicides, the approach showed severe disturbance in the synthesis of branched-chain amino acids upon treatment with imazapyr. The established technology displays a breakthrough for quantitative high-throughput plant metabolic phenotyping.

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Section: Resultsmentioning

confidence: 99%

Novel Approach for High-Throughput Metabolic Screening of Whole Plants by Stable Isotopes

Dersch

Beckers²,

Rasch³

et al. 2016

Plant Physiol.

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“…and preferably at the same time, to allow a correct calculation to be made of the δ 13 C value of the added carbon (see Rieley, 1994). The GC-C-IRMS analysis of n-alkanols and sterols as their trimethylsilyl ether derivatives has been previously reported by Jones et al (1991). For n-alkanols and n-alkanoic acids, these errors have been corrected for the contribution of the added carbon, in a similar way as that described by Rieley (1994).…”

Section: Methodsmentioning

confidence: 99%

Stable carbon isotope evidence for the microbial origin of C14C18 n-alkanoic acids in soils

Lichtfouse

Berthier

Houot

et al. 1995

Organic Geochemistry

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“…13 C values were corrected for the addition of the extra carbon atom introduced to the molecule during methylation, using a correction factor obtained by CF e EA -IRMS measurement on the derivatising methanol and application of the mass balance equation of Jones et al (1991). For details of the instrumental set up see supplementary information e S2.…”

Section: -Plfa Analysesmentioning

confidence: 99%

Functional differences in the microbial processing of recent assimilates under two contrasting perennial bioenergy plantations

Elias

Rowe

Pereira

et al. 2017

Soil Biology and Biochemistry

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a b s t r a c tLand use change driven alteration of microbial communities can have implications on belowground C cycling and storage, although our understanding of the interactions between plant C inputs and soil microbes is limited. Using phospholipid fatty acids (PLFA's) we profiled the microbial communities under two contrasting UK perennial bioenergy crops, Short Rotation Coppice (SRC) willow and Miscanthus Giganteus (miscanthus), and used Functional differences in microbial communities were highlighted by contrasting processing of labelled C. SRC willow allocated 44% of total 13 C detected into fungal PLFA relative to 9% under miscanthus and 380% more 13 C was returned to the atmosphere in soil respiration from SRC willow soil compared to miscanthus. Our findings elucidate the roles that bacteria and fungi play in the turnover of recent plant derived C under these two perennial bioenergy crops, and provide important evidence on the impacts of land use change to bioenergy on microbial community composition. Crown

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Determination of δ13C values of sedimentary straight chain and cyclic alcohols by gas chromatography/isotope ratio mass spectrometry

Cited by 98 publications

References 5 publications

Novel Approach for High-Throughput Metabolic Screening of Whole Plants by Stable Isotopes

Novel Approach for High-Throughput Metabolic Screening of Whole Plants by Stable Isotopes

Stable carbon isotope evidence for the microbial origin of C14C18 n-alkanoic acids in soils

Functional differences in the microbial processing of recent assimilates under two contrasting perennial bioenergy plantations

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