Determination of β-sitosterol and cholesterol in oils after reverse micelles with Triton X-100 coupled with ultrasound-assisted back-extraction by a water/chloroform binary system prior to gas chromatography with flame ionization detection

Kardani, Fatemeh; Daneshfar, Ali; Sahrai, Reza

doi:10.1016/j.aca.2011.05.047

Cited by 11 publications

(2 citation statements)

References 37 publications

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“…A GC-MS method has been applied to the determination of sterols aer derivatization as pentauorobenzyl esters in human plasma. 11 GC with FID, 12,13 high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, 14,15 HPLC with refractive index (RI) detection 16 and ultra high performance liquid chromatography (UHPLC)-atmospheric pressure chemical ionization (APCI)-MS for phytosterols 17 are the most frequently used methods for the analyses of sterols. A rapid development of a range of analytical platforms, including GC, HPLC, UPLC, and CE coupled to MS spectroscopy, enables separation, detection, characterization and quantitation of such metabolites and related metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of oxysterols, phytosterols and cholesterol precursors by high performance liquid chromatography tandem mass spectrometry in human serum

et al. 2013

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A fast and sensitive high performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (HPLC-APCI-MS/MS) method to identify and quantify oxysterols, phytosterols and non-cholesterol sterols at the pico-molar concentration level in human serum in only one run was developed in this study. This method allows the simultaneous separation and quantitation of individual phytosterols, cholesterol precursors and oxidized derivatives of cholesterol without a derivatization step in a single run, thus providing a more confident quantitation of sterols in serum. After saponification, solid-phase extraction (SPE) used as a clean-up step and HPLC separation, detection by MS was developed using APCI and multiple ion monitoring modes. This method employs reversed-phase C 18 SPE cartridges and serum calibrators, as well as isotopically labelled cholesterol as an internal standard added before sample processing. The time consumed for a single sample is reduced from the 4 hours of conventional sterol analysis to 1 hour including the chromatographic run time. The method has been evaluated by analyzing a certified cholesterol sample as well as by comparison to other two methods used as reference, based on GC and enzymatic reaction, respectively. Serum from 14 individuals was successfully analyzed. Detection limits for oxysterols, phytosterols and non-cholesterol sterols, all determined in a single run in small serum volumes, were between 0.47 and 1.69 pM. Intra-day precision was <7% for all sterols in inhouse-made lipoprotein-deficient serum. The limits of detection showed high sensitivity with very good intraday-and interday-precision for all sterols. The recoveries of the phytosterols, cholesterol precursors and cholestanol ranged from 77% to 92%. These analytical parameters provide a reliable and reproducible method for the identification and quantitation of human sterols.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of oxysterols, phytosterols and cholesterol precursors by high performance liquid chromatography tandem mass spectrometry in human serum

et al. 2013

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“…In most cases, the determination of compounds from complicated matrices was achieved in two steps. Initially, analytes were extracted by using solid sorbents, surfactants, or organic phases and then back extracted into a small volume of appropriate solvent compatible with the analytical instrument . In this work, extraction, clean‐up, and preconcentration were performed in one step.…”

Section: Introductionmentioning

confidence: 99%

Reversed‐phase vortex‐assisted liquid–liquid microextraction: A new sample preparation method for the determination of amygdalin in oil and kernel samples

Hosseini

Heydari

Alimoradi

2015

J of Separation Science

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A novel, simple, and rapid reversed-phase vortex-assisted liquid-liquid microextraction coupled with high-performance liquid chromatography has been introduced for the extraction, clean-up, and preconcentration of amygdalin in oil and kernel samples. In this technique, deionized water was used as the extracting solvent. Unlike the reversed-phase dispersive liquid-liquid microextraction, dispersive solvent was eliminated in the proposed method. Various parameters that affected the extraction efficiency, such as extracting solvent volume and its pH, vortex, and centrifuging times were evaluated and optimized. The calibration curve shows good linearity (r(2) = 0.9955) and precision (RSD < 5.2%) in the range of 0.07-20 μg/mL. The limit of detection and limit of quantitation were 0.02 and 0.07 μg/mL, respectively. The recoveries were in the range of 96.0-102.0% with relative standard deviation values ranging from 4.0 to 5.1%. Unlike the conventional extraction methods for plant extracts, no evaporative and re-solubilizing operations were needed in the proposed technique.

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Ringer Tablet-Based Micelle-Mediated Extraction-Solvent Back Extraction Coupled with High-Performance Liquid Chromatography for Preconcentration and Determination of Neonicotinoid Pesticides

et al. 2021

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Determination of β-sitosterol and cholesterol in oils after reverse micelles with Triton X-100 coupled with ultrasound-assisted back-extraction by a water/chloroform binary system prior to gas chromatography with flame ionization detection

Cited by 11 publications

References 37 publications

Simultaneous determination of oxysterols, phytosterols and cholesterol precursors by high performance liquid chromatography tandem mass spectrometry in human serum

Simultaneous determination of oxysterols, phytosterols and cholesterol precursors by high performance liquid chromatography tandem mass spectrometry in human serum

Reversed‐phase vortex‐assisted liquid–liquid microextraction: A new sample preparation method for the determination of amygdalin in oil and kernel samples

Ringer Tablet-Based Micelle-Mediated Extraction-Solvent Back Extraction Coupled with High-Performance Liquid Chromatography for Preconcentration and Determination of Neonicotinoid Pesticides

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