Determination of α-fetoprotein concentration based on liquid-phase binding assay using anion exchange chromatography and sulfated peptide introduced antibody

Yamagata, Yukari; Katoh, Hideo; Nakamura, Kenji; Tanaka, Takumi; Satomura, Shinji; Matsuura, Shuji

doi:10.1016/s0022-1759(98)00020-9

Cited by 46 publications

(23 citation statements)

References 10 publications

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“…[23][24][25] When total AFP concentration was less than 10 ng/mL, AFP-L3% could not be determined because of limitations in instrument sensitivity, and arbitrarily was assigned a value of 0.5%. The typical interassay variance for this test is 2.6% to 13.4%.…”

Section: Measurement Of Serum ␣-Fetoprotein Afp-l3% and Des-gamma-cmentioning

confidence: 99%

Utility of Lens culinaris Agglutinin-Reactive Fraction of α-Fetoprotein and Des-Gamma-Carboxy Prothrombin, Alone or in Combination, as Biomarkers for Hepatocellular Carcinoma

Sterling¹,

Jeffers²,

Gordon³

et al. 2009

Clinical Gastroenterology and Hepatology

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120

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Section: Measurement Of Serum ␣-Fetoprotein Afp-l3% and Des-gamma-cmentioning

confidence: 99%

Utility of Lens culinaris Agglutinin-Reactive Fraction of α-Fetoprotein and Des-Gamma-Carboxy Prothrombin, Alone or in Combination, as Biomarkers for Hepatocellular Carcinoma

Sterling¹,

Jeffers²,

Gordon³

et al. 2009

Clinical Gastroenterology and Hepatology

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120

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“…Serum AFP-L3%, which is the ratio of AFP-L3 to total AFP, and DCP were measured on an automated analyzer, called LiBASys (Liquid-Phase Binding Assay System), which is manufactured by Wako Pure Chemical Industries, Ltd., Osaka, Japan [42][43][44]. AFP was tested by an immunochemiluminescent assay (ADVIA Centaur immunoassay system; Bayer Healthcare, Tarrytown, NY, USA).…”

Section: Tumor Marker Measurementmentioning

confidence: 99%

Clinical Evaluation of Lens Culinaris Agglutinin-Reactive α-Fetoprotein and Des-γ-Carboxy Prothrombin in Histologically Proven Hepatocellular Carcinoma in the United States

Carr

Kanke

Wise³

et al. 2007

Dig Dis Sci

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116

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There is no established clinical role for the lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (AFP-L3%) and des-gamma-carboxy prothrombin (DCP) in the management of the U.S. hepatocellular carcinoma (HCC) patient population. In order to clarify the clinical usefulness and characteristics of AFP-L3% and DCP, a prospective study was performed on United States patients having histologically proven hepatocellular carcinoma. Ninety-nine histologically proven HCC patients, who were diagnosed with unresectable cancer between July 1999 and March 2001 at the Liver Cancer Center of the University of Pittsburgh Medical Center, were included for analysis. The sensitivity of AFP-L3%, DCP, and AFP was 61.6%, 72.7%, and 67.7%, respectively. The highest sensitivity, 85.9%, was obtained in the combination of three markers. Statistically significant differences were observed for portal vein invasion in AFP-L3% and AFP levels (P = 0.0059 and P = 0.0360, respectively). DCP was significantly associated with metastasis (P = 0.0368). There were significant associations between AFP-L3% and AFP results and patient survival (P = 0.0150 and P = 0.0020, respectively). AFP-L3%, platelet count,and albumin showed a significant difference with respect to outcomes on Cox's proportional hazard model (P = 0.0059, P = 0.0073, and P = 0.0265, respectively). The combination of AFP-L3%, DCP, and AFP was determined to be superior for detection of HCC compared with each marker alone or to other combinations. AFP-L3% was significantly related to portal vein invasion and patient outcomes and appears to be a useful prognostic marker for HCC.

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“…In our previous report, we demonstrated the "liquid-phase binding assay" (LBA) that separates bound and free forms by using polysulfated tyrosine peptide-modified antibody as a separation improvement molecule in HPLC [9][10][11]. It has the advantage that optimization of assay conditions such as antibody concentration can be done easily and precisely because of stoichiometric reaction in the liquid phase, resulting in good reproducibility and linear dose-response curve with wide assay range [12]. To perform liquid-phase immunoassay on chip, CE is most commonly used as a separation tool after immune reaction [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“Electrokinetic Analyte Transport Assay” for α‐fetoprotein immunoassay integrates mixing, reaction and separation on‐chip

Kawabata¹,

Wada²,

Watanabe³

et al. 2008

Electrophoresis

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A rapid and highly sensitive CE immunoassay method integrating mixing, reaction, separation, and detection on-chip is described for the measurement of alpha-fetoprotein (AFP), a liver cancer marker in blood. Antibody-binding reagents, consisting of 245-bp DNA coupled anti-AFP WA1 antibody (DNA-WA1) and HiLyte dye-labeled anti-AFP WA2 antibody (HiLyte-WA2), and AFP-containing sample were filled into adjacent zones of a chip channel defined by the laminar flow lines of the microfluidic device using pressure-driven flow. The channel geometry was thus used to quantitatively aliquot the reagents and sample into the chip. DNA-WA1 was electrokinetically concentrated in the channel and sequentially transported through the AFP-sample zone and HiLyte-WA2 zone by ITP in such a manner that the AFP sandwich immune complex formation took place in the sample and HiLyte-WA2 zones. The sandwich AFP immune complex was then detected by LIF after CGE in a separation channel that was arranged downstream of the reaction channel. AFP was detected within 136 s with a detection sensitivity of 5 pM. The on-chip immunoassay described here, applying ITP concentration, in-channel reaction, and CGE separation, has the potential of providing a rapid and sensitive method for both clinical and research applications.

show abstract

Determination of α-fetoprotein concentration based on liquid-phase binding assay using anion exchange chromatography and sulfated peptide introduced antibody

Cited by 46 publications

References 10 publications

Utility of Lens culinaris Agglutinin-Reactive Fraction of α-Fetoprotein and Des-Gamma-Carboxy Prothrombin, Alone or in Combination, as Biomarkers for Hepatocellular Carcinoma

Utility of Lens culinaris Agglutinin-Reactive Fraction of α-Fetoprotein and Des-Gamma-Carboxy Prothrombin, Alone or in Combination, as Biomarkers for Hepatocellular Carcinoma

Clinical Evaluation of Lens Culinaris Agglutinin-Reactive α-Fetoprotein and Des-γ-Carboxy Prothrombin in Histologically Proven Hepatocellular Carcinoma in the United States

“Electrokinetic Analyte Transport Assay” for α‐fetoprotein immunoassay integrates mixing, reaction and separation on‐chip

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