Determination of zinc in blood serum by electrothermal atomic absorption spectrometry with matrix modification

Accominotti, M.; Pégon, Y.; Vallon, J.J.

doi:10.1016/0009-8981(88)90247-1

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…The highest pyrolysis temperature described hitherto was 800 C, with ammonium phosphate used as the matrix modier. 12 We found that this method produced both non-linear calibrations and incomplete recovery of Zn. Welz et al 16 presented a very detailed examination on the use of a combined Mg and Pd matrix modi-er for 21 elements including Zn.…”

Section: Determination Of Pyrolysis and Atomisation Temperaturesmentioning

confidence: 86%

“…The results compared favorably with those of FAAS. Accominotti et al 12 used ammonium phosphate as a matrix modier, a 20fold dilution and Zeeman correction with a L'vov platform in the furnace, with external calibration performed using aqueous standards. Zinc has also been successfully measured in slurries of tissue samples prepared in solutions of tetramethylammonium hydroxide.…”

Section: Introductionmentioning

confidence: 99%

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Direct determination of zinc in plasma by graphite furnace atomic absorption spectrometry using palladium/magnesium and EDTA matrix modification with high temperature pyrolysis

Stevens

Hare

Volitakis

et al. 2017

J. Anal. At. Spectrom.

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High prevalence of zinc deficiency stemming from malnutrition, gastrointestinal diseases and low dietary intake accounts for detection of zinc in plasma being a frequently requested clinical pathology assay. Serum and plasma zinc determination by graphite furnace atomic absorption spectrometry (GFAAS) has previously been hampered by significant interfering species and intolerance to high pyrolysis temperatures. In this Technical Note, we report a GFAAS method developed to overcome these restrictions by employing two matrix modifiers and a high pyrolysis temperature. Serum and plasma samples were diluted twenty times with an Antifoam/Triton-X-100 diluent and measured against aqueous standards similarly diluted, without the use of Zeeman correction. Interference from chloride was eliminated using a combination of two matrix modifiers: a magnesium/palladium mixture combined with 1% (w/v) aqueous ethylenediaminetetraacetic acid (EDTA).This allowed a pyrolysis temperature of 1000 C to be used, which resulted in the complete removal of chloride interference. The accuracy of the method was verified by direct comparison with inductively coupled plasma-mass spectrometry (ICP-MS) and flame atomic absorption spectrometry (FAAS); analysis of a commercial reference material (Seronorm); and by analytical recovery studies.

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