Determination of volatile purgeable halogenated hydrocarbons in human adipose tissue and blood serum

Development of a Method for Measuring Volatile Organic Compounds in the Blood of Fire Victims Using'Purge and Trap' Gas Chromatography

“…In this study, we set as a goal to develop a simple method that would permit the detection of volatile substances characteristically found in the blood of smoke inhalation victims. As it happens, the majority of chromatographic methods described in the literature [9][10][11][12][13][14] have been directed at studying the composition of the blood in normal healthy subjects, which necessitates detection of a large number of substances at very small concentrations. Most of these techniques require a large blood specimen, concentration of the volatile substances by purge and trap and detection by mass spectroscopy.…”

“…For the majority of compounds, linearity was maintained to 100,000 ~g ~ 1-~ . For the compounds carbon tetrachloride, cyclohexane, indene, nitromethane, trichlorofluormethane which had detection thresholds above 10 ~g .1-1, calibration curves were obtained using only three concentrations (100, 1,000, and 10,000 ~g .1-1).…”

“…Among the methods most often used to measure volatile organic compounds (VOCs) is gas chromatography. The majority of the methods employ an extraction technique, such as the static head space or dynamic head space (purge and trap) methods, which offer extraction yields which are more or less elevated [9][10][11][12][13][14]. The problem with these methods is that they require a large blood sample.…”

Development of a Method for Measuring Volatile Organic Compounds in the Blood of Fire Victims Using ‘Purge and Trap’ Gas Chromatography

“…As it happens, the majority of chromatographic methods described in the literature [9][10][11][12][13][14] have been directed at studying the composition of the blood in normal healthy subjects, which necessitates detection of a large number of substances at very small concentrations. Most of these techniques require a large blood specimen, concentration of the volatile substances by purge and trap and detection by mass spectroscopy.…”

“…Among the methods most often used to measure volatile organic compounds (VOCs) is gas chromatography. The majority of the methods employ an extraction technique, such as the static head space or dynamic head space (purge and trap) methods, which offer extraction yields which are more or less elevated [9][10][11][12][13][14]. The problem with these methods is that they require a large blood sample.…”