Determination of volatile organic compounds, catechins, caffeine and theanine in Jukro tea at three growth stages by chromatographic and spectrometric methods

Jeon, Dong Bok; Hong, Young Shin; Lee, Ga Hyun; Park, Yu Min; Lee, Cheong Mi; Nho, Eun Yeong; Choi, Ji Yeon; Jamila, Nargis; Khan, Naeem; Kim, Kyong Su

doi:10.1016/j.foodchem.2016.09.184

Cited by 40 publications

(29 citation statements)

References 32 publications

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“…Many different procedures have been proposed for assay a caffeine in biological and pharmaceutical samples including gas chromatography/mass spectrometry (GC/MS) (Jeon et al 2017), liquid chromatography/mass spectrometry (LC-MS) (Li et al 2015), liquid chromatography using fluorimetric and UV detection (Ibrahim and Wahba 2014), high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) (Rybak et al 2014), ultra-high-performance liquid chromatography-tandem mass spectrometry method (UHPLC-MS/MS) (Krpo et al 2018;Liu et al 2013) and also solid surface fluorescence using membrane filters modified with MWCNTs (Talio et al 2013) or multivariate calibration-prediction techniques, principal component regression (PCR), partial least squares (PLS) and artificial neural networks (ANN) were applied to the spectrometric multicomponent analysis (Aktaş and Kitiş 2014). However, these methods are generally expensive and often require a tedious, time-consuming sample pretreatment, which makes them unsuitable for the routine analysis of large number of samples.…”

Section: Introductionmentioning

confidence: 99%

Adsorptive stripping voltammetric method for the determination of caffeine at integrated three-electrode screen-printed sensor with carbon/carbon nanofibers working electrode

2019

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A commercially available screen-printed carbon electrode coated with carbon nanofibers (SPCE/CNFs) and differentialpulse adsorptive stripping voltammetry (DPAdSV) were used to determination of caffeine. The process of electrochemical oxidation of caffeine in 0.1 mol L −1 sulfuric acid at the SPCE/CNFs surface was investigated by cyclic voltammetry (CV). The effect of the supporting electrolyte type, pH and concentration, time and potential of accumulation, amplitude and scan rate were studied to select the optimum experimental conditions. Under the optimized conditions, the well-defined caffeine peak was observed at 1.25 V (vs. screen-printed silver reference electrode) in 0.1 mol L −1 sulfuric acid. The caffeine was accumulated at − 0.8 V for 60 s. The oxidation peak current was proportional to concentration of caffeine from 2.0 × 10 −7 to 1.0 × 10 −6 mol L −1 with detection limit of 5.6 × 10 −8 mol L −1. The presented method has been successfully applied for the determination of caffeine in beverage samples. Keywords Integrated three-electrode screen-printed sensor with carbon/carbon nanofibers working electrode • Adsorptioncontrolled process • Diffusion-controlled process • Voltammetric determination of caffeine • Beverage samples This article belongs to S.I. ISSHAC10, but it reach the press at the time the special issue was published.

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Section: Introductionmentioning

confidence: 99%

Adsorptive stripping voltammetric method for the determination of caffeine at integrated three-electrode screen-printed sensor with carbon/carbon nanofibers working electrode

2019

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“…The analysis of theobromine and caffeine in foods, biological fluids, environmental samples, plants, and water, was provided with different instrumental methods, such as highperformance liquid chromatography (HPLC) [4], gas chromatography-mass spectrometry-flame ionization detection (GC-MS-FID) [5], Fourier transform-infrared spectrophotometry (FT-IR) [6], near-infrared spectroscopy [7], UV-Vis spectrophotometry [8], FT-Raman spectrometry [9], and capillary electrophoresis (CE) [10]. Of these techniques, HPLC which offers advantages, such as simplicity and selectivity coupled with a UV-Vis detector, was deemed the preferred analysis system for the analysis of theobromine and caffeine [8,11,12]. However, the co-elution of compounds is a common phenomenon when a UV-vis detector is used with HPLC [13].…”

Section: The Present Study Is Focused On the Analysis Of ------------mentioning

confidence: 99%

Determination of theobromine and caffeine in some Malaysian beverages by liquid chromatography-time-offlight mass spectrometry

Al-Qaim¹,

Yuzir²,

Mussa³

2018

Trop. J. Pharm Res

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To determine the concentration of theobromine (TB) and caffeine (CF) in tea and other beverages using liquid chromatography-time of flight-mass spectrometry (LC-TOF-MS). Methods: The extract of caffeine and theobromine from tea and other beverages was filtered by 0.45 μm nylon micro-syringe and then injected into a LC-ToF-MS system. Theobromine and caffeine were separated using Thermo Scientific C18-column (length 250 mm, width 2.1 mm and diameter 5 μm). Acetonitrile-methanol (ACN-MeOH, 3:1 v/v) was used as mobile phase B, while mobile phase A was 0.1 % FA in DIW. The volume injected was 30 μL at a rate of 0.3 mL/min. Results: Good linearity was obtained in the range of 0.3-400 and 0.2-200 mg/L for theobromine and caffeine, respectively (regression coefficient (R 2) > 0.970). The limits of detection were 0.15 and 0.05 μg/mL for theobromine and caffeine, respectively. The highest concentrations of caffeine and theobromine determined in tea samples were 159.1 and 255.8 mg/L, respectively. Conclusion: Theobromine and caffeine have been successfully analysed in tea, coffee and soft drinks. LC-TOF-MS is an accurate and promising instrument for the determination of the studied compounds in beverages.

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“…As a key branch of systems biology, metabolomics is located downstream of the other “omics” technologies and can be exploited to examine the changes that occur in living systems under different environments and obtain a direct, comprehensive, and accurate biochemical picture of the phenotype of an organism. To date, metabolomics has been used to investigate the influence of various factors on the chemical components and quality of C. sinensis leaves, including the plucking position (Lee et al., ; Zhu, Li, Zhao, Yu, & Wu, ), developmental stage (Feng et al., ; Jeon et al., ), season (Lee et al., ; Ryu et al., ; Xu, Song, Li, & Wan, ), environmental factors (Ku et al., ; Shen et al., ), habitat (Zheng, She, Xiao, Wu, & Feng, ), processing conditions (Dai et al., ; Fraser et al., ; Ku, Kim, Park, Liu, & Lee, ; Lee et al., ; Lee et al., ; Tan et al., ; Xu, Hu, Wang, Wan, & Bao, ), and cultivar (Li et al., ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Young Shoots of ‘Anji Baicha’ (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC‐MS‐Based Metabolomics

Zeng

Lin

Liu

et al. 2019

Journal of Food Science

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‘Anji Baicha’ (Camellia sinensis) is a low‐temperature‐sensitive tea variety. During the development of young shoots, the leaves of ‘Anji Baicha’ exhibit periodic albinism. The quality of ‘Anji Baicha’ is closely related to the color of the fresh leaves, with whiter leaves affording a higher amino acid content and superior sensory quality after processing. However, the metabolic mechanism of its quality formation is still unclear. In this study, we analyzed the metabolomic changes of young shoots of ‘Anji Baicha’ and screened for metabolic markers that may be involved in the periodic albinism. Positive‐ and negative‐mode UPLC‐QTOF‐MS was applied to the metabolomic analysis of young leaves of ‘Anji Baicha’ during three developmental stages (i.e., the pre‐albescent, albescent, and regreening stages). The results revealed significant differences in the metabolic profiles of the young leaves at the three stages. The differential metabolites were mainly related to the pathways of flavonoid, phenylpropanoid, and amino acid biosynthesis. The concentrations of several amino acids (primarily l‐theanine, l‐glutamate, N2‐acetyl‐l‐ornithine, l‐aspartic acid, d‐proline, l‐glutamine, l‐leucine, and pyroglutamic acid) and 12‐OPDA were significantly higher in the albescent stage. In contrast, during the albescent stages, the concentrations of several carbohydrates (d‐fructose, β‐d‐galactopyranose, 3‐O‐fucopyranosyl‐2‐acetamido‐2‐deoxyglucopyranose, galactose‐β‐1, 4‐xylose acetyl‐maltose, and 2‐fucosyllactose) were significantly lower. Moreover, catechins (mainly epigallocatechin and catechin derivatives), dimeric catechins (primarily proanthocyanidins), and flavonol and flavonol/flavone glycosides (mainly kaempferol, myricetin, quercetin, cyanidin, and delphinidin glycosides) were detected at the highest levels in the regreening or pre‐albescent stages. The obtained results enhance the current understanding of the metabolic mechanisms of periodic albinism and quality development formation in ‘Anji Baicha’. Practical Application The obtained results not only provide information regarding differential metabolites but also advance the understanding of the mechanism of periodic albinism in ‘Anji Baicha’ at the metabolite level and open up new possibilities for the genetic improvement of tea cultivars.

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Determination of volatile organic compounds, catechins, caffeine and theanine in Jukro tea at three growth stages by chromatographic and spectrometric methods

Cited by 40 publications

References 32 publications

Adsorptive stripping voltammetric method for the determination of caffeine at integrated three-electrode screen-printed sensor with carbon/carbon nanofibers working electrode

Adsorptive stripping voltammetric method for the determination of caffeine at integrated three-electrode screen-printed sensor with carbon/carbon nanofibers working electrode

Determination of theobromine and caffeine in some Malaysian beverages by liquid chromatography-time-offlight mass spectrometry

Analysis of Young Shoots of ‘Anji Baicha’ (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC‐MS‐Based Metabolomics

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