Determination of Volatile Organic Compounds and Antibacterial Activity of the Amazonian Cyanobacterium Synechococcus sp. Strain GFB01

Abstract

Cyanobacteria exhibit great biotechnological potential due to their capacity to produce compounds with various applicability. Volatile organic compounds (VOCs) possess low molecular weight and high vapor pressure. Many volatiles produced by microorganisms have biotechnological potential, including antimicrobial activity. This study aimed to investigate the VOCs synthesized by cyanobacterium Synechococcus sp. strain GFB01, and the influence of nitrate and phosphate on its antibacterial potential. The strain was isolated from the surface of the freshwater lagoon Lagoa dos Índios, Amapá state, in Northern Brazil. After cultivation, the VOCs were extracted by a simultaneous distillation-extraction process, using a Likens-Nickerson apparatus (2 h), and then identified by GC-MS. The extracts did not display inhibitory activity against the Gram-positive bacteria tested by the disk-diffusion agar method. However, the anti-Salmonella property in both extracts (methanol and aqueous) was detected. The main VOCs identified were heptadecane (81.32%) and octadecyl acetate (11.71%). To the best of our knowledge, this is the first study of VOCs emitted by a cyanobacterium from the Amazon that reports the occurrence of 6-pentadecanol and octadecyl acetate in cyanobacteria.

Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing Synechococcus sp. GFB01

“…CACIAM05 [ 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. Moreover, the influence of nitrogen on the production of volatile compounds by GFB01 has already been investigated [ 101 ]. In addition to its importance in the production of secondary metabolites, the glyceraldehyde-3-phosphate dehydrogenase-CP12-phosphoribulokinase complex, mediated by thioredoxin, proved to be protective of the enzymes of the Calvin cycle against oxidative stress [ 102 ].…”

Anabaenopeptins: What We Know So Far

“…They can inhabit several types of ecosystems, including aquatic and terrestrial. These microorganisms produce a great variety of bioactive compounds, which have been investigated mainly due to their biotechnological potential and environmental relevance [ 1 , 2 , 3 ]. Cyanotoxins are among the most studied compounds originated from cyanobacteria since they are capable of negatively affecting human and animal health [ 4 , 5 ].…”

Symbioses of Cyanobacteria in Marine Environments: Ecological Insights and Biotechnological Perspectives

“…The volatile organic compounds (VOCs) are bioactive metabolites produced by cyanobacteria and their in vitro biosynthesis is influenced by cocultivation conditions with symbiotic microorganisms. VOCs isolated from a strain of the genus Synechococcus showed antibacterial activity (50 mg mL −1 of the total extract) against the Gram-negative bacterium Salmonella typhimurium [ 259 ].…”