Determination of Volatile Constituents of Thai Fragrant Orchids by Gas Chromatography-Mass Spectrometry with Solid-Phase Microextraction

Abstract

This study was carried out to investigate the protective effect of standardized γ-oryzanol-rich extracts on oxidative DNA damage induced by Fenton reaction and antiproliferative activity against human cancer cells. Six cultivars of Thai purple rice were collected in northern Thailand. Rice bran was extracted with hexane/ethyl acetate mixture and the extract was evaporated to obtain crude rice bran oil. Each rice bran oil was further purified by column chromatography to obtain the γ-oryzanol-rich extract. The extracts contained γ-oryzanol in the range of 1.17 -7.54 % w/w, in which GAM THOR exhibited the highest γ-oryzanol content. The extr \acts containing more than 5.0 % w/w γ-oryzanol (GAM THOR, GAM DOI MUSUR and GAM SUKHOTHAI-2) were selected to be standardized with γ-oryzanol and then the protective effect on oxidative DNA damage and antiproliferative activity against four human cancer cell lines (HT-29, HCT 116, MDA-MB-468 and PC3) were investigated. The extracts (10 µg/ml) exhibited a protective effect on oxidative DNA damage induced by Fenton reaction as compared with standard quercetin (lower than 5 µg/ml). Furthermore, all of the extracts exerted antiproliferative activity against human cancer cell lines in a dose-dependent manner. GAM THOR exhibited the highest antiproliferative activity against HT-29, HCT 116, MDA-MB-468 and PC3 with an 50% inhibition concentration value of 52.18 ± 1.21, 40.58 ± 5.69, 48.59 ± 2.40 and 51.61 ± 1.30 µg/ml, respectively. From these findings, γ-oryzanolrich extracts from Thai purple rice bran show potential as chemopreventive supplements or in nutraceuticals.

Volatile Fingerprint of Italian Populations of Orchids Using Solid Phase Microextraction and Gas Chromatography Coupled with Mass Spectrometry

“…The literature reports that only some hydrocarbons, in particular very long-chain alkanes and alkenes and terpenes act as chemical mimicry of the sex pheromone of the virgin female pollinators. In agreement with our results, other authors confirmed terpenes and hydrocarbons together with alcohols and aldehydes as major constituents of orchid volatile profiles [10,11,19,20] and among these chemical classes a high emission of nonanal and caryophyllene by orchid flowers was previously reported [19,21,22]. In particular, the chemical composition of orchids pollinated by bees (e.g., Ophrys ) revealed an abundance of terpenes (mono- and sesquiterpenes) [23].…”

Estimation of drug-likeness properties of GC–MS separated bioactive compounds in rare medicinal Pleione maculata using molecular docking technique and SwissADME in silico tools

“…harrisonianum Holtt., Vanda coerulea and Dendrobium parishii Rchb . F., (Julsrigival et al 2013 ) and Dendrophylax lindenii (Sadler et al 2011 ). In this paper, the GC–MS report of Pleione maculata mainly focused on total medicinal important compounds present in different parts.…”

The Diurnal and Nocturnal Floral Scent of Dracaena fragrans (L.) Ker Gawl. in Thailand

“…Volatile compounds of the flower samples were extracted using the method described by Julsrigival et al (2013). Briefly, an AOC 5000 Combi PAL SPME holder (CTC Analytic AG., Switzerland) was equipped with a 65-µm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber assembly (Supelco, Bellfonte, PA).…”