Determination of Vitamin K in Serum Using HPLC with Post-Column Reaction and Fluorescence Detection

Lambert, Willy E.; Leenheer, A.P. De; Lefevere, M.F.

doi:10.1093/chromsci/24.2.76

Cited by 32 publications

(7 citation statements)

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“…Vitamin K I was measured in 1 -mL serum samples by a two-step HPLC procedure, according to the method of Lambert et al (7,8). A few modifications were applied.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Chromosome Aberrations and Sister Chromatid Exchanges in Peripheral Blood Lymphocytes of Newborns after Vitamin K Prophylaxis at Birth

et al. 1991

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ABSTRACT. In many countries vitamin K prophylaxis at birth is recommended to prevent bleeding in infants due to vitamin K deficiency. Because the incidence of clinical vitamin K deficiency is very low, such a vitamin K administration should be completely safe. However, an increase in sister chromatid exchanges in lymphocytes of fetal sheep 24 h after injection of vitamin K, has been reported. Therefore, a study concerning genotoxicity of vitamin K, in man was conducted. Sister chromatid exchanges and chromosome aberrations were analyzed in peripheral blood lymphocytes of six newborns 24 h after intramuscular administration of 1 mg vitamin K, and in six control neonates. The mean number of sister chromatid exchanges per metaphase in the vitamin K group was 8.88 f 1.22 as compared with 9.05 f 1.14 in the control group (

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“…Vitamin K I was measured in 1 -mL serum samples by a two-step HPLC procedure, according to the method of Lambert et al (7,8). A few modifications were applied.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Chromosome Aberrations and Sister Chromatid Exchanges in Peripheral Blood Lymphocytes of Newborns after Vitamin K Prophylaxis at Birth

et al. 1991

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“…This method offered more selectivity than the traditional chick bioassay commonly used to measure vitamin K status; however, its sensitivity was still insufficient. More recent methods include electrochemical techniques, fluorescence detection after postcolumn reduction, − and gas chromatography-mass spectrometry with HPLC . These techniques provide greater selectivity and sensitivity than UV detection.…”

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confidence: 99%

Method for the Determination of Vitamin K Homologues in Human Plasma Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry

et al. 2004

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We report here the development of a precise and sensitive method for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4), and menaquinone-7 (MK-7) in human plasma using HPLC-tandem mass-mass spectrometry with atmospheric pressure chemical ionization (LC-APCI-MS/MS). The method involves the use of stable isotope (18)O-labeled internal standard compounds, which were synthesized in our laboratory, and the selection of a precursor and product ion with a MS/MS multiple reaction monitoring method. The average intraassay and interassay variation values for PK, MK-4, and MK-7 were <10%. Average spiked recoveries from authentic compounds added to normal human plasma samples for PK, MK-4, and MK-7 were 98-102%. Mean plasma concentrations of PK, MK-4, and MK-7 from healthy subjects (n = 20) were 1.22 +/-0.57, 0.39 +/- 0.46, and 6.37 +/- 7.45 ng/mL, respectively. We conclude that this novel LC-APCI-MS/MS method should be useful for the evaluation of vitamin K status in postmenopausal women and elderly subjects and provides useful information for the treatment and prevention of osteoporosis with vitamin K.

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“…Participating laboratories were Shearer et al (1982) 11.0 (6) NA NA UV Ueno and Suttie (1983) NA NA Electrochemical EC-oxidative Langenberg and Tjaden (1984) 2.1 (5) 3.2 (10) Electrochemical Fluorescence Kusube et al (1984) NA NA Electrochemical Fluorescence Haroon et al (1984) NA NA Electrochemical EC-oxidative Hart et al (1984) 8.9 (6) NA NA EC-reductive Hart et al (1985) NA 10 (6) Electrochemical EC-oxidative Haroon et al (1986) 6.0 (12) 12.0 (12) Zinc Fluorescence Hirauchi et al (1986) NA NA Electrochemical Fluorescence Lambert et al (1986) 3.6 (5 asked to analyse the EQA samples during a specified calendar month and to submit their results to the scheme organizers before the end of the assigned analysis period. Participants subsequently received reports summarizing their analytical performance.…”

Section: Experimental Program and Protocolmentioning

confidence: 99%

The external quality assurance of phylloquinone (vitamin K₁) analysis in human serum

Card

Shearer

Schurgers

et al. 2009

Biomedical Chromatography

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The vitamin K external quality assurance scheme (KEQAS) aims to assist in the harmonization of phylloquinone (vitamin K(1)) analysis in order to improve the comparability of clinical and nutritional studies. Serum samples were despatched to 17 groups from eight countries during 2000-2006. Using pilot data (1996-1999), an analytical performance target of 20% absolute difference from the all-laboratory trimmed mean (ALTM) was assigned and formed the basis for interlaboratory comparison. Assay specificity, analytical bias and assay performance were evaluated. From 21 batches of samples distributed, 414 results were reported of which 2.7% were outliers. The mean interlaboratory absolute difference from the ALTM was 21.7% with 47% of groups consistently meeting the performance target. The mean interlaboratory coefficient of variation was 29.6%. The false positive rate for phylloquinone depleted samples was high at 35%. Bias was found to be independent of HPLC-detector type (fluorescence vs electrochemical). Assay characteristics for the measurement of phylloquinone in human serum compare favourably with methods for analytes at equivalent concentrations. The high proportion of false positive results suggest that poor assay specificity at low phylloquinone concentrations is a common problem, which in the clinical setting could lead to underreporting of vitamin K deficiency.

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Determination of Vitamin K in Serum Using HPLC with Post-Column Reaction and Fluorescence Detection

Cited by 32 publications

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Analysis of Chromosome Aberrations and Sister Chromatid Exchanges in Peripheral Blood Lymphocytes of Newborns after Vitamin K Prophylaxis at Birth

Analysis of Chromosome Aberrations and Sister Chromatid Exchanges in Peripheral Blood Lymphocytes of Newborns after Vitamin K Prophylaxis at Birth

Method for the Determination of Vitamin K Homologues in Human Plasma Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry

The external quality assurance of phylloquinone (vitamin K₁) analysis in human serum

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Determination of Vitamin K in Serum Using HPLC with Post-Column Reaction and Fluorescence Detection

Cited by 32 publications

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Analysis of Chromosome Aberrations and Sister Chromatid Exchanges in Peripheral Blood Lymphocytes of Newborns after Vitamin K Prophylaxis at Birth

Analysis of Chromosome Aberrations and Sister Chromatid Exchanges in Peripheral Blood Lymphocytes of Newborns after Vitamin K Prophylaxis at Birth

Method for the Determination of Vitamin K Homologues in Human Plasma Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry

The external quality assurance of phylloquinone (vitamin K1) analysis in human serum

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The external quality assurance of phylloquinone (vitamin K₁) analysis in human serum