Determination of Vitamin B12 in Milk Products and Selected Foods by Optical Biosensor Protein-Binding Assay: Method Comparison

Abstract

Biomolecular interaction analysis was evaluated for the automated determination of vitamin B12 in a range of foods. The analytical technique was configured as a biosensor-based, nonlabeled inhibition protein-binding assay using nonintrinsic R-protein. Sample extraction conditions were optimized, and both ligand specificity and nonspecific binding considerations were evaluated. Performance parameters included a quantitation range of 0.08–2.40 ng/mL, recoveries of 89–106%, agreement against assigned reference values for 3 independent certified food reference materials, and a mean between-laboratory reproducibility relative standard deviation of 4.9%. The proposed method was compared with reference microbiological and radioisotope protein-binding methods for a range of food samples. A wide selection of milks, infant formulas, meats, and liver were evaluated for their vitamin B12 content. The influence of season was studied in herd milk, early lactation was followed for a single animal, and the cobalamin content of bovine, caprine, and ovine milks was compared.

Evaluation of Micronutrient Sensors for Food Matrices in Resource‐Limited Settings: A Systematic Narrative Review

“…After independent full-text review, the reviewers met to exclude articles (n = 41) after reaching a final agreement for the following reasons: matrix is not food or potable water (n = 32), no interference study was conducted (n = 2), no dynamic range or limit of detection were studied (n = 4), and previously reported sensors in prior studies (n = 3). A total of 42 sensors (Aksuner, Henden, Yenigul, Yilmaz, & Cukurovali, 2011;Alexander, Dimitrakopoulos, & Hibbert, 1997 Hosseini et al, 2010;Indyk et al, 2000;Indyk et al, 2002;L. Kong et al, 2016;Kumar et al, 2014;Lermo et al, 2009;W.…”

A survey of the year 2002 commercial optical biosensor literature

“…Of the 473 articles that mentioned which Biacore instrument was used, 48% of researchers used Biacore 2000, 26% used Biacore 3000, 15% used Biacore X, and 10% used Biacore 1000. Three research groups used the newly released Biacore Q, a platform designed specifically for food analysis, 701,704,705,707 while two used Biacore J 199,720 and one used the Bialite instrument. 547 Because of the large number of articles describing Biacore-based studies, these are subdivided in the reference list by application.…”

“…While Biacore technology is still employed primarily to study proteins, antibodies, receptors, peptides, 399-466 and oligonucleotides, it is increasingly used to characterize extracellular matrix components, 548-590 small molecules, 591-627 lipids, 628-671 carbohydrates and self-assembled monolayers. [717][718][719][720][721][722][723][724] In addition, biosensors are now more commonly used in food science, [700][701][702][703][704][705][706][707][708][709] in combination with mass spectrometry, [710][711][712][713][714][715][716] in clinical studies, [725][726][727][728][729][730] and in studying complex biological systems such as fibrils and cells. [731][732][733][734][735][736][737][738][739] Forty-seven articles described studies performed using Affinity Sensors' IAsys instruments (www.affinity-sensors.…”

Fast and Efficient RP-HPLC Method for Simultaneous Determination of Water-Soluble Vitamins in Some Nutraceutical Supplements

“…However, in case of real samples that may include vitamins and proteins, adsorption to the electrode surface is a serious problem [6]. On the other hand, the common Official analytical method is non-specific, tedious and time consuming as these methods involve pretreatment of the sample through physical, biological and complex chemical reactions to eliminate the interferences found [7]; it followed by individual methods for each different vitamin. The determinations of vitamins B and vitamin C in food were performed by spectrophotometry [8], electrochemical method [9,10], capillary electrophoresis [11].…”