Determination of Vitamin A in Liver

Gallup, Willis D.; Hoefer, J. A.

doi:10.1021/i560153a005

Cited by 60 publications

(13 citation statements)

References 8 publications

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“…Liver vitamin A was determined by the method of Gallup and Hoefer (1946), and plasma vitamin E by the procedure of Bieri et al (1964). Plasma Ca, P and Mg, and liver Mg, were determined by previously noted procedures.…”

Section: Methodsmentioning

confidence: 99%

Evaluating the Nutritional Status of Beef Cattle Herds from Four Soil Order Regions of Florida. I. Macroelements, Protein, Carotene, Vitamins A and E, Hemoglobin and Hematocrit

Kiatoko

McDowell

Bertrand

et al. 1982

Journal of Animal Science

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The nutrient status of grazing beef cattle from four selected soil order regions of Florida was examined. Liver, blood, hair and feces samples from 14 heifers and 14 cows, plus forage samples, were collected during two periods of the year from nine ranches located in four different regions. Soil samples were collected during one period. The soil order regions were the Histosol (southeast), Spodosol (southwest), Entisol (central) and Ultisol (northwest). Mean forage P values were higher (P less than .05) in the wet season, while mean hair P levels were higher (P less than .05) during the dry season. Mean forage, plasma, liver and hair Mg values were higher (P less than .05) in the wet season. Plasma vitamin E, liver vitamin A and forage carotene levels were higher (P less than .05) during the wet season. Mean forage P content was deficient (less than .25%) during both seasons and varied from .10% in the dry season to .16% during the wet season. Mean forage Mg (less than .18%) and K (less than .60%) concentrations were deficient in the dry season. Forage protein was deficient (less than 7.0%) in five of seven ranches during the dry season. Extractable soil Ca, exchangeable soil Ca, Mn, Al, H+, soil organic matter and effective cation exchange capacity were higher (P less than .05) in the Histosol region. Extractable soil K was low (60 ppm) in all regions, except the northwest. Forage P was critical (less than .25%) during the dry season in all regions and varied from .08 to .15%, while plasma P was deficient (4.5 mg/100 ml) in animals from the southeast during the dry season only.

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Section: Methodsmentioning

confidence: 99%

Evaluating the Nutritional Status of Beef Cattle Herds from Four Soil Order Regions of Florida. I. Macroelements, Protein, Carotene, Vitamins A and E, Hemoglobin and Hematocrit

Kiatoko

McDowell

Bertrand

et al. 1982

Journal of Animal Science

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“…The carotene and vitamin A in the liver were analyzed by a slightly modified Gallup and Hoefer (1946) method. Fifty percent water solution of KOH was added to the alcohol in each sample rather than previously mixing the KOH and alcohol.…”

Section: Methodsmentioning

confidence: 99%

The Development of Chicks Fed Different Levels of Sun-Cured and Dehydrated Alfalfa and the Vitamin A and Carotene Storage in their Livers

Mangelson¹,

Draper²,

Greenwood³

et al. 1949

Poultry Science

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“…potassium hydroxide. The suspension was then refluxed until a clear solution was obtained (10 to 15 minutes) and the vitamin A extracted into light petroleum using a method similar to that described by Gallup and Hoeffer (1946) for liver samples. Vitamin A was estimated spectrophotometrically in the light petroleum solutions (concentrated where necessary by evaporation at room temperature under vacuum in a stream of nitrogen) using a Beckman Model D U photoelectric spectrophotometer and applying a three point correction procedure to allow for extraneous absorption (McGillivray 1950).…”

Section: Experimental (A) In Vitro Conversion Experimentsmentioning

confidence: 99%

The Conversion of Carotene to Vitamin a in Sheep and Cattle

Wa¹

1951

Aust. Jnl. Of Bio. Sci.

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SummaryIt has been established that the conversion of carotene to vitamin A in the sheep occurs in the wall of the intestine. This conclusion is based on surviving tissue experiments in which sections of intestine have been incubated with carotene and the product conclusively identified as vitamin A by colorimetric and spectrophotometric methods. It is further supported by the high vitamin A levels in intestinal as compared with non-intestinal lymph, and similar observations with cattle suggest the intestine as the site of conversion in this species also.

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Determination of Vitamin A in Liver

Cited by 60 publications

References 8 publications

Evaluating the Nutritional Status of Beef Cattle Herds from Four Soil Order Regions of Florida. I. Macroelements, Protein, Carotene, Vitamins A and E, Hemoglobin and Hematocrit

Evaluating the Nutritional Status of Beef Cattle Herds from Four Soil Order Regions of Florida. I. Macroelements, Protein, Carotene, Vitamins A and E, Hemoglobin and Hematocrit

The Development of Chicks Fed Different Levels of Sun-Cured and Dehydrated Alfalfa and the Vitamin A and Carotene Storage in their Livers

The Conversion of Carotene to Vitamin a in Sheep and Cattle

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