Determination of vanillin and related flavor compounds in natural vanilla extracts and vanilla-flavored foods by thin layer chromatography and automated multiple development

Investigating the discrimination potential of linear and nonlinear spectral multivariate calibrations for analysis of phenolic compounds in their binary and ternary mixtures and calculation pKa values

“…Vanillin (VA) is one of main aromatic flavor compounds in natural vanilla derived from Vanilla planifolia [1]. VA is readily available from low cost synthetic procedures based on eugenol, guaiacol and from lignin-containing sulfite liquor, a byproduct of wood pulp processing in paper manufacture [2]. The flavor profile of vanilla contains N 200 components, of which vanillic acid (VAI) and syringaldehyde (SIA) are other flavor compounds founded in vanilla.…”

Ultrafast UPLC‐ESI‐MS and HPLC with monolithic column for determination of principal flavor compounds in vanilla pods

“…The principal components accounted for the most desirable flavor characteristics in authentic natural vanilla extracts are p-hydroxybenzoic acid (1), vanillic acid (2), p-hydroxybenzaldehyde (3), and vanillin (4). The ratio of 1, 2, and 3 to vanillin is generally considered an index of quality of natural vanilla extracts and is used for the determination of origin as well as possible adulteration of vanilla extracts [4,5].…”

Methoxybenzaldehydes in Plants:Insight to the Natural Resources, Isolation, Application and Biosynthesis

“…On the other hand, separation of vanillin has remained an area of interest in food and cosmetic industries for decades. Though numerous quality methods are available for separation and quantification of compounds from vanilla extracts, including TLC, HPTLC (high performance thin layer chromatography), isotope ratio MS and HPLC, the search continues for a simple and rapid HPLC-based method for isolation of this fragrant methoxybenzaldehyde from ethanolic vanilla pod extract [23][24][25][26][27][28] . A RP-HPLC-based method was developed for efficient separation of compounds using which maximum number of compounds, including vanillin has been efficiently separated in a single run using RP Purospher ®-Star RP-18e column and a photodiode array detector 29 .…”