Determination of Urinary Uroporphyrin by a Direct Extraction Method

Dresel, E. I. B.; Rimington, C.; Tooth, B. E.

doi:10.3109/00365515609049247

Cited by 35 publications

(9 citation statements)

References 12 publications

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“…(1). Recently the method ehborated by Dresel et al (5). and described by Rimington (25), has been adopted for uroporphyrin determination in urines containing small amounts of porphyrins.…”

Section: The Control Groupmentioning

confidence: 99%

“…The uroporphyrin values given above refer to urines in which ether soluble porphyrins were not extracted before the determination. In urines with small amounts of porphyrins the method of Dresel et al (5) was also used (values within brackets in Table VI) for the determination of uroporphyrin. In urines from eight patients studied 2 to 3 I/: years after the last phlebotomy, the uroporphyrin content determined according to Drssel et al (5) ranged from 0 to 124 rig124 h, and values within the normal range (0 to 25 pg/24 h) were encountered in four of them.…”

Section: Table VI Porphyrin Excretion In Phlebotomy-treated Patientsmentioning

confidence: 99%

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The Effect of Phlebotomy Therapy in Porphyria Cutanea Tarda

Lundvall

1971

Journal of Internal Medicine

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Abstract. Porphyrin excretion and clinical signs have been studied in 21 patients with porphyria cutanea tarda treated by phlebotomy and in a control series of 12 patients. Phlebotomy was performed until signs of depleted iron stores appeared. Clinical and biochemical remission occurred in every phlebotomy‐treated patient. In 18 patients who were bled frequently (usually 0.5 1 per week) and who were not given iron supplements in conjunction with phlebotomy, the urinary porphyrin excretion decreased in all to a level of 1 mg per day or less within 1/2 year. Remission ensued in patients with iron overload as well as in patients with quantitatively normal iron stores and in patients abstaining from alcohol as well as in patients with a persistent abuse of alcohol. A decrease in porphyrin excretion comparable to that of phlebotomy‐treated patients occurred in only two out of the 12 control patients. There was a significant relationship between the period required until iron stores were depleted and until the urinary porphyrin excretion fell to 1 mg per day. In one patient given iron supplement for 6 1/2 months in conjunction with phlebotomy, more blood had to be removed to obtain a remission than in any other patient, and this patient had the longest treatment (16 months). Thus the results have shown that phlebotomy therapy is effective in porphyria cutanea tarda. It seems probable that the effect is mediated through the phlebotomy‐induced reduction of iron stores.

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“…(1). Recently the method ehborated by Dresel et al (5). and described by Rimington (25), has been adopted for uroporphyrin determination in urines containing small amounts of porphyrins.…”

Section: The Control Groupmentioning

confidence: 99%

Section: Table VI Porphyrin Excretion In Phlebotomy-treated Patientsmentioning

confidence: 99%

The Effect of Phlebotomy Therapy in Porphyria Cutanea Tarda

Lundvall

1971

Journal of Internal Medicine

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“…aliquot was extracted to yield an ethersoluble coproporphyrin fraction in 0.15 N-HCl, and a small, ether-insoluble fraction of uroporphyrin. The latter fraction was further extracted by cyclohexanone at pH 1.5. and finally taken into 0.5 N-HCl (Dresel, Rimington and Tooth, 1956). Apart from allowing the urine to stand for 24 hours after collection, no additional steps for conversion of porphyrinogens were undertaken (Watson, de Mello, Schwartz, Hawkinson and Bossenmaier, 1951;Zondag and van Kampen, 1956).…”

Section: Urinary Porphyrinsmentioning

confidence: 99%

Refractory Normoblastic Anaemia: A Clinical and Haematological Study of Seven Cases

et al. 1959

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“…Porphyrin metabolism was investigated using the following methods: quantitative urine porphyrin (Rimington and Sveinsson, 1950;Dresel et al, 1956); A.L.A. and P.B.G.…”

Section: Methodsmentioning

confidence: 99%