Determination of urinary trans,trans-muconic acid using molecularly imprinted polymer in microextraction by packed sorbent followed by liquid chromatography with ultraviolet detection

Developing a Method for Determination of Urinary Delta-Amino-Levulinic Acid using Molecularly Imprinted Polymers

“…A lower mass of adsorber is followed by reducing the volume of the solvent; in addition, the substrate of the absorber is not lost and compared to disposable cartridges of solid phase extraction (SPE), which can be used more than 100 times depending on the sample [13]. Various absorbents such as C2, C8, C18, SCX, SAX, APS, SDVB, HDVB are used in MEPS, but the lack of selectivity, the main disadvantage of these adsorbents, has led researchers to use high-selectivity adsorbents such as MIPs [14]. Therefore, considering the advantages of the MEPS compared to conventional SPE sorbents, as well as the highly selective and low cost of producing MIPs, their combination can be described as a simple, fast, selective, high-sensitivity and environmentfriendly method and for use in preparing environmental, pharmaceutical, toxicological, and food samples [15].…”

“…In the current study, we developed a novel approach based on MIP-MEPS to determine U-ALA. According to our knowledge, MIP-MEPS analysis has been carried out by analytical methods like GC, HPLC, LC-MS-MS, and GC-MS [14][15][16][17][18][19]. So far this method has not been performed with a spectrophotometer and for the first time, MIP-MEPS was combined with the spectrophotometric method with a microcell to analyze urinary U-ALA.…”

A Nanoprobe Based on Gated Mesoporous Silica Nanoparticles for The Selective and Sensitive Detection of Benzene Metabolite t,t‐Muconic Acid in Urine

“…However,these analytical methods are usually high costs, need trainedp ersonnel,a re not suitable for in situ measurements and use hazardous organic solvents. [16][17][18][19][20][21][22][23][24][25][26] For these reasons, the development of reliable, selective and sensitivem ethods for the rapid and in situ detection of t,t-MA is of importance.A tt his respect, very recently, the use of nanostructured materials for the detection of benzene and other aromatic compounds has been reported. [27,28] From another point of view,a zacryptands are supramolecular metal complexes that are constituted by an organic core (azacryptand) with two coordinated metal ions.…”

“…Ta king into account the high selectivity found and the low limit of detection of S4 toward t,t-MA (see Table S5 for ac omparisonoft he sensing features of S4 with the traditional methods reported in the literature [18][19][20][21][22][23][24][25][26] ), we take as tep forward and evaluated possible use of the sensing nanoparticles to determine t,t-MA in urine. For this purpose, urine was spiked with known amounts of t,t-MA and the concentration was determined using S4 nanoparticles by meanso facalibration curve in the same media( limit of detection of 0.017 mm fort ,t-MA in urine was determined).…”

Microextração de canabinoides em urina usando dispositivo empacotado com polímero molecularmente impresso e análise por cromatografia líquida - espectrometria de massas sequencial

“…Dessa forma, para a extração dos analitos, a amostra é aspirada para dentro da seringa, antes passando pela fase extratora, onde os analitos são sorvidos em um processo similar ao da SPE. 45,46 Entretanto 48,49 No caso da aplicação de MIPs como fase sorvente para MEPS, existem alguns estudos publicados nos últimos anos, entre eles: determinação de ácido trans,trans-mucônico em amostras de urina; 50 Após a realização da etapa otimizada de preparo de amostra por MEPS, todas essas soluções de urina processadas e fortificadas foram empregadas na validação do método analítico.…”