Determination of urinary oxalate with commercially available oxalate oxidase.

Buttery, J E; Ludvigsen, N; Braiotta, E A; Pannall, P R

doi:10.1093/clinchem/29.4.700

Cited by 38 publications

(6 citation statements)

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“…The plasma and urine concentration of creatinine was measured using an autoanalyzer. Urine oxalate was measured according to Buttery et al [16] using oxalate oxidase and HPLC in the same assay; glycolate was determined using spinach glycolic acid oxidase. Urine concentration of urea was measured by using a kinetic test with urease and glutamate dehydrogenase (Cobas Integra 700).…”

Section: Methodsmentioning

confidence: 99%

Vitamin B6 metabolites in idiopathic calcium stone formers: no evidence for a link to hyperoxaluria

Kaelin

Casez

Jaeger

2004

Urological Research

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Vitamin B6 metabolites and their potential correlates to urinary oxalate excretion in idiopathic calcium stone formers (ICSF) compared with healthy subjects were investigated. This clinical study was performed in a population of male ICSF with (Hyperoxalurics, n=55) or without hyperoxaluria (Normooxalurics, n=57) as well as in 100 healthy male control subjects. Pyridoxal 5'-phosphate serum concentration (S-pyridoxal 5'P) and 24-h urinary excretion of 4-pyridoxic acid (U-4pyridoxic acid) were measured using HPLC; 24-h urinary excretion of oxalate (U-oxalate) was measured concurrently. A subgroup of subjects (40 Hyperoxalurics, 15 Normooxalurics and 50 controls) underwent the same measurements before and after 7-day pyridoxine loading per os (pyridoxine hydrochloride, 300 mg/d). Under usual conditions, U-4pyridoxic acid was similar in the three groups, whereas mean S-pyridoxal 5'P was significantly lower ( p<0.0001) in the Hyperoxalurics (59.6+/-21.2 nmol/L) and in the Normooxalurics (64.9+/-19.7 nmol/L) than in the controls (86.0+/-31.0 nmol/L). No correlation could be found between U-oxalate and U-4pyridoxic acid or S-pyridoxal 5'P. After B6 loading, S-pyridoxal 5'P was still significantly lower in the Hyperoxalurics (415+/-180 nmol/L, p<0.001) and in the Normooxalurics (429+/-115 nmol/L, p=0.036) than in the controls (546+/-180 nmol/L), although there was no difference between groups for U-4pyridoxic acid. No correlation in any group could be found between changes in U-oxalate and changes in U-4pyridoxic acid or S-pyridoxal 5'P. Although there is no vitamin B6 deficiency in ICSF with or without hyperoxaluria, these patients, on average, have lower levels of S-pyridoxal 5'P than healthy subjects. However, this slight decrease does not seem to account for idiopathic hyperoxaluria.

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Section: Methodsmentioning

confidence: 99%

Vitamin B6 metabolites in idiopathic calcium stone formers: no evidence for a link to hyperoxaluria

Kaelin

Casez

Jaeger

2004

Urological Research

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“…A number of analytical methods such as enzymatic assays, 7 , 8 ion chromatography, 9 gas chromatography (GC), 10 high‐performance liquid chromatography (HPLC), 11 , 12 , 13 , 14 and liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) 15 , 16 have been reported for the analysis of urinary oxalate. Among these, enzymatic assays are used by the majority of the laboratories, but a manual sample clean‐up step with pH adjustment and activated charcoal columns is always required due to color inhibition caused by reducing substrates.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a high‐performance liquid chromatography method for urinary oxalate determination and investigation regarding the pediatric reference interval of spot urinary oxalate to creatinine ratio for screening of primary hyperoxaluria

Shen

Luo

et al. 2021

Clinical Laboratory Analysis

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Background Urinary oxalate can provide important clues for the screening and monitoring of children with primary hyperoxaluria (PH), which is a potentially life‐threatening condition. However, little effort has been devoted to improve the oxalate assay in recent years. We have proposed a reliable and cost‐effective high‐performance liquid chromatography (HPLC) method for urinary oxalate determination. Methods Urine specimens were centrifuged after one‐step derivatization, and the supernatants were subjected to HPLC analysis. Results The method was validated with consistent linearity from 0.0625 to 2.0 mmol/L with coefficients of variation ≤7.73%, good recovery, low carryover, satisfactory sample stability, and analytical specificity. The lower limit of quantification and the limit of detection were 0.03130 and 0.0156 mmol/L, respectively. Imprecision values were ≤2.92% and ≤16.6% for externally and internally produced controls, respectively. The pediatric reference interval of spot urinary oxalate to creatinine ratios was established together with its application in screening of PH in patients with renal diseases, revealing its successful deployment in our laboratory. Conclusions This reliable HPLC method could serve as a significant tool to determine urinary oxalate levels for screening and monitoring of children with PH in routine clinical laboratories.

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“…1 Among the variety of methodologies, such as volumetric analysis, precipitation, solvent extraction, and mass spectrometry etc., the enzymatic method is one of the most widely used methods in the majority of clinics and laboratories as routine oxalate analyzers due to its high specificity and sensitivity. [2][3][4][5] However, at least two different enzymes are required in one single enzymatic assay, in which one enzyme is used to decompose oxalate, and the other is used to monitor the oxalate decomposition products, H 2 O 2 or CO 2 . Accordingly, the cost of the enzymatic method is high and the detection procedure is complicated.…”

Section: Introductionmentioning

confidence: 99%

A novel urinary oxalate determination method via a catalase model compound with oxalate oxidase

et al. 2010

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Determination of urinary oxalate with commercially available oxalate oxidase.

Cited by 38 publications

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Vitamin B6 metabolites in idiopathic calcium stone formers: no evidence for a link to hyperoxaluria

Vitamin B6 metabolites in idiopathic calcium stone formers: no evidence for a link to hyperoxaluria

Evaluation of a high‐performance liquid chromatography method for urinary oxalate determination and investigation regarding the pediatric reference interval of spot urinary oxalate to creatinine ratio for screening of primary hyperoxaluria

A novel urinary oxalate determination method via a catalase model compound with oxalate oxidase

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