Determination of urinary myo-inositol concentration by an improved enzymatic cycling method using myo-inositol dehydrogenase from Flavobacterium sp.

Determination of Myo-inositol and d-chiro-inositol in black rice bran by capillary electrophoresis with electrochemical detection

“…1). Until now a range of analytical methods have already been published for the determination of myo-inositol, D-chiro-inositol or inositol phosphates: microbiological assay (Wood and Stanway, 2001;Ono et al, 2003;Yamakoshi et al, 2003), high-performance liquid chromatography (HPLC) , gas chromatography (GC) , ion chromatography (IC) (Tagliaferri et al, 2000) and capillary electrophoresis (CE) (Bennani and Fabre, 2001). Among these methods, GC requires a time-consuming pre-column derivatization step to increase the volatility of inositol.…”

Determination of myo-inositol in biological samples by liquid chromatography–mass spectrometry

“…Enzymatic methods [8][9][10][11] use a myo-inositol dehydrogenase to oxidize myo-inositol. This reaction is NAD + -dependent and NADH can be reoxidized with oxalacetate and malate dehydrogenase, measuring the resultant malate fluorimetrically [8].…”

“…This reaction is NAD + -dependent and NADH can be reoxidized with oxalacetate and malate dehydrogenase, measuring the resultant malate fluorimetrically [8]. Myo-inositol dehydrogenase can also be screened [10]. If reoxidation of NADH is performed with iodonitrotetrazolium chloride and diaphorase, the resultant formazan can be measured spectrophotometrically [11].…”

Identification of membrane-bound quinoprotein inositol dehydrogenase in Gluconobacter oxydans ATCC 621H

“…Furthermore, myo-inositol dehydrogenases can be used in enzymic assays for diagnosis of diabetes in its early stages (Yamakoshi et al, 2003).…”