Determination of urinary catecholamines and metanephrines in cardiac deaths

Hervet, Tania; Grouzmann, Eric; Grabherr, Silke; Mangin, Patrice; Palmière, Cristian

doi:10.1007/s00414-015-1303-2

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…Diagnosis of death due to hypothermia is usually reliant on signs such as frost-erythema and Wischnewski's spots, which are present in approximately two thirds of cases [89]. Biochemical indications of antemortem cold stress may include raised urinary adrenaline and noradrenaline, and/or metanephrine (metadrenaline) concentrations [4,90,91]. Urinary catecholamine measurement has been recommended in preference to blood analysis because of postmortem catecholamine release from sympathetic nerve endings and the adrenal glands [92], but catecholamine instability is a major issue as with clinical catecholamine measurement and of course urine may not be available postmortem.…”

Section: Hypothermia/hyperthermiamentioning

confidence: 99%

Postmortem biochemistry: Current applications

Belsey

Flanagan

2016

Journal of Forensic and Legal Medicine

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The results of biochemical analyses in specimens obtained postmortem may aid death investigation when diabetic and alcoholic ketoacidosis is suspected, when death may have been the result of drowning, anaphylaxis, or involved a prolonged stress response such as hypothermia, and in the diagnosis of disease processes such as inflammation, early myocardial infarction, or sepsis. There is often cross-over with different disciplines, in particular with clinical and forensic toxicology, since some endogenous substances such as sodium chloride, potassium chloride, and insulin can be used as poisons. The interpretation of results is often complicated because of the likelihood of postmortem change in analyte concentration or activity, and proper interpretation must take into account all the available evidence. The unpredictability of postmortem changes means that use of biochemical measurements in time of death estimation has little value. The use of vitreous humour is beneficial for many analytes as the eye is in a physically protected environment, this medium may be less affected by autolysis or microbial metabolism than blood, and the assays can be performed with due precaution using standard clinical chemistry analysers. However, interpretation of results may not be straightforward because (i) defined reference ranges in life are often lacking, (ii) there is a dearth of knowledge regarding, for example, the speed of equilibration of many analytes between blood, vitreous humour, and other fluids that may be sampled, and (iii) the effects of post-mortem change are difficult to quantify because of the lack of control data. A major limitation is that postmortem vitreous glucose measurements are of no help in diagnosing antemortem hypoglycaemia.

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Section: Hypothermia/hyperthermiamentioning

confidence: 99%

Postmortem biochemistry: Current applications

Belsey

Flanagan

2016

Journal of Forensic and Legal Medicine

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“…Однак, вивчаючи результати дослідження, можна виявити суперечливі результати. Тривалий час вважали, що їх підвищення в сироватці крові свідчить про короткий агональний період, натомість як при тривалій агонії їх рівень знижується [25,26,27]. Натомість Wilke N. зі співавторами (2007) при дослідженні співвідношення адреналін/ норадреналін в крові із серця, стегнової вени, ліквору, сечі та скловидному тілі не виявили суттєвих відмінностей при раптовій та агональній смерті [28].…”

Section: The Role Of Post-mortem Biochemistry In the Forensic Medical...unclassified

THE ROLE OF POST-MORTEM BIOCHEMISTRY IN THE FORENSIC MEDICAL DIAGNOSIS OF MECHANICAL INJURY (literature review)

Ергард

Біляков

2023

Med. Sci. of Ukr.

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Background. Mechanical trauma occupies one of the leading places in forensic medical examination. The main task of the expert, when conducting this kind of examination, is to provide a scientifically based answer to the questions of the pre-trial investigation bodies or the court, which relate not only to the determination of the mechanism of the injury, but also to the time period of its occurrence. Traditional methods of morphological research and methods of laboratory analyzes cannot always provide comprehensive information, especially in cases of determining the age of mechanical trauma in the early Post-mortem period. Therefore, new methods of biochemical research are being actively introduced into forensic medical practice. Aim: Analysis of the literature devoted to the application of various biochemical diagnostic methods in forensic medical practice to establish the antiquity of mechanical trauma. Results. In pathological conditions, such as, for example, trauma, certain molecular changes occur in the human body, which are associated with a systemic reaction to a stress factor and are manifested before the appearance of morphological changes in tissues and organs. Therefore, the use of biochemical research methods will significantly expand the possibilities of forensic diagnostics. The publication shows promising directions for the application of the biochemical method for forensic trauma diagnosis. Issues of establishing the statute of limitations for mechanical injuries are considered. Today, scientific studies of the body's stress state in cases of fatal injuries are becoming more and more common. Which is of great importance for forensic medical expert practice. Therefore, the role of manifestations of the body's systemic response to trauma is described. The role of natriuretic peptides, creatine kinase and procalcitonin in diagnosing the duration of the traumatic process is also shown. Conclusion. The relevance and practical significance of biochemical research for the forensic diagnosis of the time of occurrence of a mechanical injury, as well as the development of new diagnostic criteria for determining the body's systemic response to trauma, necessitated a deep and comprehensive study of the above problems.

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“…Although there are few reports in the available literature regarding the assessment of the relationship between the level of selected neurotransmitters and cardiovascular diseases, a few studies confirm this relationship. For example, the results obtained by Hervet et al suggest that stress situations result in a massive release of catecholamines, which can be examined after death by E and NE measurements in post-mortem serum, vitreous humor and urine, as well as in pericardial fluid and CSF [158]. Increased serum concentrations of both E and NE have been reported in myocardial infarction deaths [159].…”

Section: Clinical Importance Of the Determination Of Bas In Human mentioning

confidence: 99%

Recent Trends in the Quantification of Biogenic Amines in Biofluids as Biomarkers of Various Disorders: A Review

Plenis

Olędzka

Kowalski

et al. 2019

JCM

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Biogenic amines (BAs) are bioactive endogenous compounds which play a significant physiological role in many cell processes like cell proliferation and differentiation, signal transduction and membrane stability. Likewise, they are important in the regulation of body temperature, the increase/decrease of blood pressure or intake of nutrition, as well as in the synthesis of nucleic acids and proteins, hormones and alkaloids. Additionally, it was confirmed that these compounds can be considered as useful biomarkers for the diagnosis, therapy and prognosis of several neuroendocrine and cardiovascular disorders, including neuroendocrine tumours (NET), schizophrenia and Parkinson’s Disease. Due to the fact that BAs are chemically unstable, light-sensitive and possess a high tendency for spontaneous oxidation and decomposition at high pH values, their determination is a real challenge. Moreover, their concentrations in biological matrices are extremely low. These issues make the measurement of BA levels in biological matrices problematic and the application of reliable bioanalytical methods for the extraction and determination of these molecules is needed. This article presents an overview of the most recent trends in the quantification of BAs in human samples with a special focus on liquid chromatography (LC), gas chromatography (GC) and capillary electrophoresis (CE) techniques. Thus, new approaches and technical possibilities applied in these methodologies for the assessment of BA profiles in human samples and the priorities for future research are reported and critically discussed. Moreover, the most important applications of LC, GC and CE in pharmacology, psychology, oncology and clinical endocrinology in the area of the analysis of BAs for the diagnosis, follow-up and monitoring of the therapy of various health disorders are presented and critically evaluated.

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Determination of urinary catecholamines and metanephrines in cardiac deaths

Cited by 7 publications

References 24 publications

Postmortem biochemistry: Current applications

Postmortem biochemistry: Current applications

THE ROLE OF POST-MORTEM BIOCHEMISTRY IN THE FORENSIC MEDICAL DIAGNOSIS OF MECHANICAL INJURY (literature review)

Recent Trends in the Quantification of Biogenic Amines in Biofluids as Biomarkers of Various Disorders: A Review

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