Determination of urinary androstanediol and testosterone in normal men by gas—liquid chromatography

Charransol, G.; Bobas-Masson, F.; Mauvais-Jarvis, P

doi:10.1016/s0021-9673(01)82927-7

Cited by 11 publications

(4 citation statements)

References 9 publications

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“…Androstanediol in the urine was assayed by gas chromatography as already described (Charransol, Bobas-Masson, Guillemant & Mauvais-Jarvis, 1972). Blank values were constantly 0.…”

Section: Methodsmentioning

confidence: 99%

Androgen Production and Skin Metabolism in Hirsutism

Kuttenn¹,

Mowszowicz²,

Schaison³

et al. 1977

Journal of Endocrinology

199

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The concentrations of testosterone, androstenedione and dihydrotestosterone (DHT) in the plasma and 5\g=a\-androstane-3\g=a\,17\g=b\-diol(androstanediol) in the urine were measured in 40 women with hirsutism of ovarian, adrenal and idiopathic origin. Conversion of [3H]testosterone to DHT, 3\g=a\-and 3\g=b\-androstanediols was also studied in homogenates of pubic skin obtained from 15 of the patients. Results were compared with values obtained from normal men and women.Values for the levels of testosterone, DHT and androstenedione in the plasma and androstanediol in the urine of hirsute women were all above control levels, especially for plasma androstenedione and urinary androstanediol (P < 0\m=.\001). This finding was particularly marked in patients with hirsutism of ovarian origin. Conversion of [3H]testosterone to 5\g=a\-reducedmetabolites by homogenates of skin obtained from hirsute women was significantly greater than by homogenates of skin from normal women (P < 0\m=.\001) but was the same as the value for normal men. The highest values for conversion were obtained from the patients with idiopathic hirsutism.These results indicate that androstenedione is the principal androgen secreted in hirsutism. In sexual skin this steroid may be converted to DHT and 3\g=a\-, and 3\g=b\-androstanediols and the increased activity of testosterone 5\g=a\-reductase may result in an exaggerated 'utilization' of androstenedione in this tissue. The high rate of excretion of androstanediol in the urine of patients with idiopathic hirsutism may be explained by the fact that this steroid is an end-product of testosterone metabolism. * Present address and address for reprint requests: Hôpital Necker, 149 rue de Sèvres, 75730 Paris Cedex 15, France.

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“…Androstanediol in the urine was assayed by gas chromatography as already described (Charransol, Bobas-Masson, Guillemant & Mauvais-Jarvis, 1972). Blank values were constantly 0.…”

Section: Methodsmentioning

confidence: 99%

Androgen Production and Skin Metabolism in Hirsutism

Kuttenn¹,

Mowszowicz²,

Schaison³

et al. 1977

Journal of Endocrinology

199

View full text Add to dashboard Cite

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“…Urinary 3a-androstanediol and testosterone were estimated by gas chromatography as described by Charransol et al (1972). Urinary 3a-androstanediol and testosterone were estimated by gas chromatography as described by Charransol et al (1972).…”

Section: In Vitro Assaysmentioning

confidence: 99%

TESTOSTERONE 5α-Reduction IN THE SKIN OF NORMAL SUBJECTS AND OF PATIENTS WITH ABNORMAL SEX DEVELOPMENT

Kuttenn

Mauvais-Jarvis

1975

Acta Endocrinologica

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Human pubic skin was obtained from normal subjects and patients with abnormal sex differentiation. Skin samples (200 mg) supplemented with NADPH, were incubated for 1 h with labelled testosterone. The conversion of testosterone to dihydrotestosterone1), 3\g=a\-and 3\g=b\-androstanediol was calculated. This conversion averaged 14.9 \m=+-\ 3.4 % (se) in 11 normal men and 3.6 \ m=+-\1.4 % (se) in 8 normal women. In 4 children as in 4 young hypogonadotrophic hypogonadal men, the conversion rate of testosterone to 5\g=a\-reducedmetabolites was low (0.8 to 3.5%) and increased at puberty (13.5 to 19.2%). After administration of HCG for 3 months to 1 of the hypogonadal men, it reached 30.2 %. Inversely, the formation of dihydrotestosterone and androstanediols from testosterone was suppressed in 2 men treated with large doses of oestrogen. In 3 subjects with an incomplete form of testicular feminization syndrome, the conversion rate of testosterone to 5\g=a\-reducedmetabolites was in the normal male range (6.4 to 18.3%), whereas it was low in one case of the complete form of the syndrome (1.5%). In 9 women with idiopathic hirsutism the rate of 5\g=a\-reducedmetabolites recovered from testosterone was close to that of normal men (13.5 \ m=+-\5.5% (se). From theseresults, it is postulated that in human subjects, there is a good correlation between hair growth in skin from a sexual area and the extent of testosterone 5\g=a\-reductionin this tissue. Such an enzymatic activity 0 The following trivial names have been used: Dihydrotestosterone = 17/?-hydroxy-5aandrostan-3-one; androstenedione = 4-androstene-3,17-dione; 3ci-androstanediol = 5aandrostane-3«,17/?-diol; 3/î-androstanediol = 5«-androstane-3/5\17/?-diol; androstanediols = 3a-androstanediol + 3/i-androstanediol.

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“…Gaz-sıvı kromatografisinde % 3 SE-30, yüksek sıcaklık derecelerine dayanıklılığı ve steroidlerin daha iyi ayrılmasını sağladığından seçilmiştir (23,24). Testosteron ve epitestosteron gaz kromatografisine doğrudan verildiklerinde SE-30 fazında iyi bir şekilde ayrılmaları müm-kün olmamıştır.…”

Section: Tartişma Ve Sonuçunclassified

“…Testosteron ve epitestosteron ile doğrudan çalışıldığında 0.2 µg madde tanımlanabilmesine karşın asetillemeden sonra 0.05 µg madde düzeyine inilebilmiştir. Elde ettiğimiz duyarlılık sınırı bu konudaki araştırmalarla uyum göstermektedir (15,23). Şekil-1'de testosteron asetat ve epitestosteron asetat standartlarının gaz-sıvı kromatografisindeki kromatogramları gösterilmiştir.…”

Section: Tartişma Ve Sonuçunclassified

İdrarda Testosteron ve Epitestosteronun İnce Tabaka Kromatografisi ve Gaz Sıvı Kromatografisi İle Tayini

Vural¹,

Süzen²

1989

Ankara Universitesi Eczacilik Fakultesi Dergisi

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ÖZETİdrarda testosteron (T) ve epitestosteronun (E) tayini için, ince tabaka kromatografisi ile kombine basit ve duyarlı gaz kromatografik bir yöntem tanımlanmıştır. İdrardaki testosteron ve epitestosteron asit hidrolizi ve ekstraksiyondan sonra ince tabaka kromatografisi (İTK) ile ayrılmış ve elüe edilmiştir. Elüat asetillenmiş, tekrar İTK ile izole edilmiş ve iç standart ilave edilip gaz sıvı kromatografisinde (GSK) analiz edilmiştir. Bu yöntemle verim testosteron için % 73.79 ± 3.9 ve epitestosteron için % 75.94 ± 7.9, duyarlılık ise 0.05 µg olarak bulunmuştur. Geliştirilen yöntem normal erkek ve kadın idrarları ile ilaç verilen kişilerin idrarlarına uygulanarak T E oranlan saptanmış-tır. Normal erkek idrarlarında bu oran 1.96 ± 0.62, normal kadın idrarlarında 2.62 ± 0.32, ilaç verilen kişilerin idrarlarında ise 4.88 ± 1.78 olarak bulunmuştur. Bulgular doping kontrolü açısından değerlen-dirilmiştir. SUMMARYA simple and accurate gas chromatographic method for the determination of testosterone (T) and epitestosterone (E) in human urine is described. Urinary testosterone and epitestosterone after acid hydrolysis and extraction , were separated by thin layer chromatography (TLC)

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Determination of urinary androstanediol and testosterone in normal men by gas—liquid chromatography

Cited by 11 publications

References 9 publications

Androgen Production and Skin Metabolism in Hirsutism

Androgen Production and Skin Metabolism in Hirsutism

TESTOSTERONE 5α-Reduction IN THE SKIN OF NORMAL SUBJECTS AND OF PATIENTS WITH ABNORMAL SEX DEVELOPMENT

İdrarda Testosteron ve Epitestosteronun İnce Tabaka Kromatografisi ve Gaz Sıvı Kromatografisi İle Tayini

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