Determination of urinary adenosine using resonance light scattering of gold nanoparticles modified structure-switching aptamer

Zhang, Jin-Quan; Wang, Yongsheng; He, Yan; Jiang, Tao; Yang, Hongmei; Xuan, Tan; Kang, Rong-Hui; Yuan, Yukun; Shi, Lin-Fei

doi:10.1016/j.ab.2009.10.027

Cited by 60 publications

(11 citation statements)

References 38 publications

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“…The assembly process involving the formation of G-quartets could induce AuNPs aggregation and produce an amplified RLS signal. Thus, this method could detect nanomolar adenosine [167]. Other aptamer-functionalized AuNPs for targeting breast cancer cells using light scattering was reported by Chang's group.…”

Section: Other Detection Methodsmentioning

confidence: 84%

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Wang

2010

Bioanal Rev

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Gold nanoparticles (AuNPs) exhibit many predominant capabilities such as high biocompatibility, chemical stability, strong localized surface plasmon resonance absorption, and high extinction coefficient in the visible region. These properties have enabled the extensive use of AuNPs in optical and electrochemical biosensors. As a kind of functional nucleic acids, aptamers can be considered as a valid alternative to antibodies or other bio-receptors and have been widely employed to develop novel biosensors. We are summarizing here the state of the art of AuNP-based biosensors that use functional aptamers as molecular recognition elements. In many cases, AuNPs themselves can be used as a probe for detection, such as various colorimetric aptasensors and fluorescent aptasensors. They also can be used as probe vectors to enlarge detection signals and to increase the number of conceivable substrates in electrochemical aptasensors.

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Section: Other Detection Methodsmentioning

confidence: 84%

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Wang

2010

Bioanal Rev

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“…Au-NPs were prepared according to a previously reported method with a slight modification [32,33]. In detail, solutions of HAuCl 4 and trisodium citrate were filtered through a 0.8 μm microporous membrane filter prior to use.…”

Section: Methodsmentioning

confidence: 99%

Quartz Crystal Microbalance Aptasensor for Sensitive Detection of Mercury(II) Based on Signal Amplification with Gold Nanoparticles

Dong

Zhao

2012

Sensors

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We show that a short mercury-specific aptamer (MSA) along with gold nanoparticles (Au-NPs) can be used to determine Hg(II) ion by a combination of a QCM-based sensor and a flow system. The MSA binds specifically to Hg(II), and the Au-NPs can amplify the signal to enhance sensitivity. Specifically, the short thiolated MSAs are immobilized on the surface of the QCM as the capture probe, and the MSAs are linked to the Au-NPs as the linking probe. The two components can form a sandwich structure of the T-Hg(II)-T type in the presence of Hg(II) ions. This leads to change in the mass on the QCM and a change in the resonance frequency. Hg(II) can be determined with a detection limit of 0.24 ± 0.06 nM which is better by three orders of magnitude than previous methods. The sensor can be regenerated by disrupting the T-Hg(II)-T base pairs with a solution of cysteine.

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“…By using a carboxylated dextran film to eliminate nonspecific adsorption of ODNcapped Au NPs, a signal amplification method has been designed to show a remarkable detection limit of DNA as low as 2.1×10 −20 mol, corresponding to 1.38 fM [19]. By combining the specific recognition with signal amplification of adenosine aptamer (Apt) coupled with Au NPs via G-quartetinduced nanoparticle assembly, a sensitive resonance light scattering (RLS) detection method for adenosine in human urine has been reported [20]. The use of gold development results in greater signal enhancement than the typical silver development, and multiple rounds of metal development have been found to increase the resulting signal compared to one development [21].…”

Section: Nanoparticle-amplified Optical Assaymentioning

confidence: 99%

Sensitive biosensing strategy based on functional nanomaterials

2011

Sci. China Chem.

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The first decade of the 21st century has been labeled as "the sensing decade". The functional nanomaterials offer excellent platforms for fabrication of sensitive biosensing devices, including optical and electronic biosensors. A lot of works have focused on the biofunctionalization of different nanomaterials, such as metal nanoparticles, semiconductor nanoparticles and carbon nanostructures, by physical adsorption, electrostatic binding, specific recognition or covalent coupling. These biofunctionalized nanomaterials can be used as catalysts, electronic conductors, optical emitters, carriers or tracers to obtain the amplified detection signal and the stabilized recognition probes or biosensing interface. The designed signal amplification strategies have greatly promoted the development of stable, specific, selective and sensitive biosensors in different fields. This review introduces some novel principles and detection strategies in the area of biosensing, based on functional nanomaterials. The general methods for biofunctionalization of nanomaterials with biomolecules and their biosensing application in immunoassay of protein, DNA detection, carbohydrate analysis and cytosensing are also described.

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Determination of urinary adenosine using resonance light scattering of gold nanoparticles modified structure-switching aptamer

Cited by 60 publications

References 38 publications

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Quartz Crystal Microbalance Aptasensor for Sensitive Detection of Mercury(II) Based on Signal Amplification with Gold Nanoparticles

Sensitive biosensing strategy based on functional nanomaterials

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