Determination of uric acid in biological fluids by high-pressure liquid chromatography

Milner, John A.; Perkins, E. G.

doi:10.1016/0003-2697(78)90456-6

Cited by 24 publications

(4 citation statements)

References 12 publications

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“…Therefore, the apparent effects of the higher doses of several xanthines needed to be corrected when their vehicle was DMSO ≥ 2220 mM, while no correction was required for the lower concentrations of this organosulfur compound. In spite of its recommended use for the dilution of uric acid [38], NaOH was not further used as a vehicle since it interfered with the fluorescence signal ( Figure S2b), and enhanced the signal attenuation induced by uric acid itself. Ethanol is recommended at 10% v/v for preparing IBMX stock solutions and did not exhibit similar inhibition, even when used as a vehicle up to a final level of 20% v/v (Figure 1).…”

Section: Influence Of Increasing Doses Of Vehicles On Primary Amine Omentioning

confidence: 99%

Methylxanthines Inhibit Primary Amine Oxidase and Monoamine Oxidase Activities of Human Adipose Tissue

et al. 2020

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Background: Methylxanthines including caffeine and theobromine are widely consumed compounds and were recently shown to interact with bovine copper-containing amine oxidase. To the best of our knowledge, no direct demonstration of any interplay between these phytochemicals and human primary amine oxidase (PrAO) has been reported to date. We took advantage of the coexistence of PrAO and monoamine oxidase (MAO) activities in human subcutaneous adipose tissue (hScAT) to test the interaction between several methylxanthines and these enzymes, which are involved in many key pathophysiological processes. Methods: Benzylamine, methylamine, and tyramine were used as substrates for PrAO and MAO in homogenates of subcutaneous adipose depots obtained from overweight women undergoing plastic surgery. Methylxanthines were tested as substrates or inhibitors by fluorimetric determination of hydrogen peroxide, an end-product of amine oxidation. Results: Semicarbazide-sensitive PrAO activity was inhibited by theobromine, caffeine, and isobutylmethylxanthine (IBMX) while theophylline, paraxanthine, and 7-methylxanthine had little effect. Theobromine inhibited PrAO activity by 54% at 2.5 mM. Overall, the relationship between methylxanthine structure and the degree of inhibition was similar to that seen with bovine PrAO, although higher concentrations (mM) were required for inhibition. Theobromine also inhibited oxidation of tyramine by MAO, at the limits of its solubility in a DMSO vehicle. At doses higher than 12 % v/v, DMSO impaired MAO activity. MAO was also inhibited by millimolar doses of IBMX, caffeine and by other methylxanthines to a lesser extent. Conclusions: This preclinical study extrapolates previous findings with bovine PrAO to human tissues. Given that PrAO is a potential target for anti-inflammatory drugs, it indicates that alongside phosphodiesterase inhibition and adenosine receptor antagonism, PrAO and MAO inhibition could contribute to the health benefits of methylxanthines, especially their anti-inflammatory effects.

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Section: Influence Of Increasing Doses Of Vehicles On Primary Amine Omentioning

confidence: 99%

Methylxanthines Inhibit Primary Amine Oxidase and Monoamine Oxidase Activities of Human Adipose Tissue

et al. 2020

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“…To avoid these interferences, many HPLC methods were developed. Ion-exchange [16][17][18], ion-pair [19,20], reversed phase [21,22] and size-exclusion chromatography [23] were used for UA determination. The main problem of most of these methods is the need of deproteination of serum samples before analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of uric acid in urine, saliva and calcium oxalate renal calculi by high-performance liquid chromatography/mass spectrometry

Perelló

Sanchís

Gráses

2005

Journal of Chromatography B

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“…A few studies in the 1980s were published for quantifying uric acid by anion‐exchange chromatography coupled with UV detection. Milner and Perkins reported a method employing a laboratory‐packed column with strong anion‐exchange resin and potassium chloride (pH 7.4) as eluent [19]. Their assay had an upper detection limit of 1.4 μg and a calibration range from 0.1 to 140 mg/L.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an ion chromatography‐ultraviolet method for determination of uric acid in the delactosed permeate

Tian

Ormond²,

Saini

et al. 2023

Separation Science Plus

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In this study, we developed and validated a new analytical method for the quantitative evaluation of uric acid in delactosed permeate using ion chromatography coupled with an ultraviolet detector. Uric acid was extracted from delactosed permeate samples by diluted hydroxide solution at room temperature. The separation was carried out on a high‐capacity anion‐exchange column by isocratic elution with 50 mM potassium hydroxide. Uric acid was detected at 295 nm. The results from this study demonstrate that the new ion chromatography‐ultraviolet method facilitates both the resolution and the detection of uric acid and provides a highly accurate and sensitive determination of uric acid in delactosed permeate samples with minimal sample preparation effort. The new method provided an excellent range of analyte response linearity in the 2.5–255.6 ng range with R2 > 0.99. The limit of detection for uric acid obtained was 0.04 mg/kg in 1 mM sodium hydroxide. Intraday and interday variabilities were less than 3%, and the mean recoveries (%) of the uric acid spiked in two types of delactosed permeate were between 100.4% and 103.9%. This method was successfully applied to determine uric acid amounts in ten delactosed permeate samples.

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Determination of uric acid in biological fluids by high-pressure liquid chromatography

Cited by 24 publications

References 12 publications

Methylxanthines Inhibit Primary Amine Oxidase and Monoamine Oxidase Activities of Human Adipose Tissue

Methylxanthines Inhibit Primary Amine Oxidase and Monoamine Oxidase Activities of Human Adipose Tissue

Determination of uric acid in urine, saliva and calcium oxalate renal calculi by high-performance liquid chromatography/mass spectrometry

Development and validation of an ion chromatography‐ultraviolet method for determination of uric acid in the delactosed permeate

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