Determination of Uric Acid in Human Urine by Ion‐exclusion Chromatography with UV Detection Using Pure Water as Mobile Phase

Abstract

A simple, rapid and accurate ion‐exclusion chromatographic method coupled with a UV detector for the determination of uric acid in human urine samples has been developed. The separation was carried out on an ion‐exclusion column using only pure water as mobile phase. The detection wavelength was 254 nm and urine sample was injected directly without any pretreatment. Furthermore, the retention behavior of uric acid on the ion‐exclusion column was researched when pure water and 1 mmol·L−1 HCl were used as mobile phase, respectively. The stability of uric acid was also further investigated within 28 days. In this method, the linear range of the calibration curve for uric acid was 0.25–100 mg·L−1, and the detection limit calculated at S/N=3 was 0.02 mg·L−1. The proposed ion‐exclusion chromatographic method has been used for the determination of uric acid in human urine.

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“…The concentrations of UA in plasma and urine are usually used as valuable indicator for health. Therefore, for the diagnosis of patients suffering from a range of disorder associated with altered purine metabolism, a simple, sensitive, and rapid analytical method for the quantitative analysis of UA is essential for clinical and biomedical fields 31,32 . This review has highlighted the significant developments in rapid and alternative techniques for the detection of UA in recent years.…”

The Determination of Uric Acid in Human Saliva by Liquid Chromatography with Electrochemical Detection

“…UV detection at 254 nm. [18] Uric acid Gout patients' plasma and urine 0.5 µg/mL 5 to 200 µg/mL Microemulsion electrokinetic chromatography. [19] Uric acid Human urine 0.5 µg/L 0.10 to 20 mg/L Ion chromatography with conductivity detection.…”

La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells

“…Considering its importance and abundance in the human body, it is no wonder that numerous methods have been developed for UA detection and determination. The separation and detection methods commonly involve chromatography or electrophoresis coupled with UV/VIS [ 6 , 7 , 8 , 9 , 10 ] spectroscopy or electrochemical detection (voltammetry [ 11 , 12 , 13 , 14 , 15 , 16 ], ECL [ 17 , 18 , 19 , 20 ], and amperometry [ 21 , 22 , 23 , 24 , 25 ]), including both enzymatic and nonenzymatic approaches [ 26 ]. Recently, attention has shifted toward electrochemical sensors and biosensors because they enable the fast, direct, and precise determination of the analyte in the complex biological matrix.…”