Determination of Uric Acid in Canine Serum and Urine By High Performance Liquid Chromatography

Felice, Lawrence J.; Dombrovskis, Dzintra; LaFond, Elizabeth; Bartges, Joseph W.; Osborne, Carl A.

doi:10.1111/j.1939-165x.1990.tb00549.x

Cited by 6 publications

(1 citation statement)

References 6 publications

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“…Urine purine metabolite : creatinine ratios from voided urine samples are used as a reliable screening tool to diagnose human beings with genetic mutations (Duran and others 1997, Zuo and others 2008) and gout (Moriwaki and others 2001). HPLC has been identified as a reliable tool to detect uric acid in the urine of dogs (Felice and others 1990). Uric acid: creatinine ratios in spot urine samples have been used to qualitatively identify hyperuricosuric dogs with a mutation of the urate transporter SLC2A9 (Karmi and others 2010) and these results were consistent with previous uric acid measurement via 24‐hour urine collections (Bartges and others 1999).…”

Section: Discussionmentioning

confidence: 99%

The effect of disease on the urinary purine metabolite concentrations in dogs

Rivara¹,

Johnson

Lulich³

et al. 2013

Veterinary Record

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This prospective study was designed to determine the urinary concentrations of purine metabolites in healthy and diseased dogs. The goals were to test the hypothesis that urine concentrations of terminal purine metabolites will identify dogs with diseases that disturb purine degradation. Five hundred and sixty-three client-owned dogs admitted sequentially to the veterinary medical centre were included. Dogs were divided into groups on the basis of their disease. Urine concentrations of xanthine, uric acid, allantoin and creatinine were measured by high-pressure liquid chromatography. Xanthine and uric acid to creatinine ratios were significantly increased in dogs with chronic kidney disease (p = 0.01). The uric acid to creatinine ratio was significantly increased in dogs with cancer compared with clinically healthy dogs (p = 0.04), and significantly increased in dogs receiving chemotherapy for their disease (p < 0.01). Compared to clinically healthy dogs, xanthine and uric acid to creatinine ratios were significantly increased in dogs with hyperadrenocorticism (p < 0.01, and 0.04, respectively). Therefore, the results of this study found that the urinary concentrations of purine metabolites in dogs are significantly impacted by systemic disease. Cancer, chronic kidney disease, and hyperadrenocorticism are associated with altered concentrations of urinary purine metabolites in dogs.

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Section: Discussionmentioning

confidence: 99%

The effect of disease on the urinary purine metabolite concentrations in dogs

Rivara¹,

Johnson

Lulich³

et al. 2013

Veterinary Record

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show abstract

Silver nanoparticles plasmon resonance-based method for the determination of uric acid in human plasma and urine samples

Amjadi

Rahimpour

2012

Microchim Acta

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Antioxidant Status of Pair-Fed Labrador Retrievers Is Affected by Diet Restriction and Aging

Stowe

Lawler²,

Kealy³

2006

The Journal of Nutrition

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Twenty-four sibling pairs of 8-wk-old Labrador Retrievers were assigned to an experiment to determine the effects of diet restriction (75% of control-fed pair mate) on the quality and span of canine life and to identify biological markers of aging in dogs. The antioxidant status of these dogs was monitored by annual assays for serum retinol (RT), retinyl palmitate (RP), total vitamin A (VA), vitamin E (VE), selenium (Se), copper (Cu), and ceruloplasmin (Cp), plasma ascorbic acid (AA), uric acid (UA), and total peroxyl-radical trapping activity (TRAP), and whole-blood glutathione peroxidase (Gpx). Data in this report are for the 6-y period of the experiment when the dogs were between 5 and 10 y of age. Diet restriction reduced RT, VE, Cu, and Cp. Aging was associated with decreased RP, VA, VE, Se, and Cu and with increased RT, Cp, and Gpx. Female dogs had lower RP, VA, Cu, and Cp than male dogs. Litter effects were observed for VE, Cu, UA, and Gpx. Treatment effects on serum RT and Cu suggest that these variables are not as regulated homeostatically by hepatic storage as in most other species. Although the antioxidant profiles did not elucidate how diet restriction contributes to longevity, they have the potential to enhance our understanding of canine clinical nutrition and to have practical applications in formulating canine diets.

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Determination of Uric Acid in Canine Serum and Urine By High Performance Liquid Chromatography

Cited by 6 publications

References 6 publications

The effect of disease on the urinary purine metabolite concentrations in dogs

The effect of disease on the urinary purine metabolite concentrations in dogs

Silver nanoparticles plasmon resonance-based method for the determination of uric acid in human plasma and urine samples

Antioxidant Status of Pair-Fed Labrador Retrievers Is Affected by Diet Restriction and Aging

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