1981
DOI: 10.1016/0009-8981(81)90158-3
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Determination of urate concentration by ultraviolet light absorbance change produced on oxidation with alkaline ferricyanide

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Cited by 5 publications
(2 citation statements)
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“…Oxidation of urate and ascorbate was monitored at 37°C by continuous recording of the absorbance at UV absorption maxima of 292 nm for urate and of 268 nm for ascorbate [20,28,29], using a Kontron (Uvikon 933) double-beam spectrophotometer equipped with a 12 position automated sample changer. Measurements were carried out in quartz cuvettes containing 146 mM NaCl and a 3.3-mM sodium phosphate-buffered solution (PBS), pH 7.4 (final volume of 1.5 ml, optical pathway 1 cm).…”
Section: Monitoring Copper-induced Oxidation Of Urate and Ascorbatementioning
confidence: 99%
“…Oxidation of urate and ascorbate was monitored at 37°C by continuous recording of the absorbance at UV absorption maxima of 292 nm for urate and of 268 nm for ascorbate [20,28,29], using a Kontron (Uvikon 933) double-beam spectrophotometer equipped with a 12 position automated sample changer. Measurements were carried out in quartz cuvettes containing 146 mM NaCl and a 3.3-mM sodium phosphate-buffered solution (PBS), pH 7.4 (final volume of 1.5 ml, optical pathway 1 cm).…”
Section: Monitoring Copper-induced Oxidation Of Urate and Ascorbatementioning
confidence: 99%
“…The determination of the serum and urinary uric acid concentration was performed using our modification of Morin's original procedure [17]. The serum level of Ap and its active metabolite, alloxanthine, was measured using our own method [16].…”
Section: Methodsmentioning
confidence: 99%