Determination of tylosins A, B, C and D in bee larvae by liquid chromatography coupled to ion trap-tandem mass spectrometry

Bernal, José; Martín, M.T.; Toribio, Laura; Martín-Hernández, Raquel; Higes, Mariano; Nozal, Marı́a J.

doi:10.1016/j.jchromb.2011.03.052

Cited by 12 publications

(7 citation statements)

References 34 publications

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“…24 for both TC and CTC in multi-specie animal tissue and detected in a real beef sample at 6.0 µg kg -1 . The lowest LOD was obtained for TYL at 0.010 µg kg -1 which was orders of magnitude lower than previously reported 15 . This result confirmed the observation that TYL had the highest S/N ratio from Fig.…”

Section: Methods Validationcontrasting

confidence: 70%

A rapid and sensitive LC-MS/MS method for the determination of multi-class residues of antibiotics in chicken liver

Sichilongo¹,

Muckoya²,

Nindi³

2015

S.Afr.j.chem

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Section: Methods Validationcontrasting

confidence: 70%

A rapid and sensitive LC-MS/MS method for the determination of multi-class residues of antibiotics in chicken liver

Sichilongo¹,

Muckoya²,

Nindi³

2015

S.Afr.j.chem

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“…Similar to the study by Reynaldi et al (2017) , TS is used by dusting methods in Japan, and only 150 mg of TS is used for 10,000 adult bees. The dose of TS in Japan is lower than and almost equivalent to those in the studies by Reynaldi et al (2017) and Bernal et al (2011) , respectively; therefore, the TS concentration in young larvae is expected to be less than 1–4 μg/ml. In contrast, MICs of TS for DTK384ermC and DTK384pJ18TS1mac were 8–16 μg/ml, suggesting that ermC -positive P. larvae is clinically resistant to TS.…”

Section: Discussionmentioning

confidence: 85%

“…According to the article, the mean concentration of TS in 2-day-old larvae measured by microbiological assay with Geobacillus stearothermophilus ATCC 12980 was always less than 4 μg/ml ( Reynaldi et al, 2017 ). In another previous study, honey bee colonies containing around 30,000 worker bees were fed 500 g of synthetic honey with 200 mg or 400 mg of TS for 48 h, and the maximum concentration of TS in 48-h-old larvae was less than 1 μg/g even when treated with 400 mg of TS ( Bernal et al, 2011 ). Similar to the study by Reynaldi et al (2017) , TS is used by dusting methods in Japan, and only 150 mg of TS is used for 10,000 adult bees.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Genes in Bacteria Isolated From Japanese Honey, and Their Potential for Conferring Macrolide and Lincosamide Resistance in the American Foulbrood Pathogen Paenibacillus larvae

et al. 2021

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American foulbrood (AFB) is the most serious bacterial disease of honey bee brood. Spores of the causative agent Paenibacillus larvae are ingested by bee larvae via brood foods and germinated cells proliferate in the larval midgut. In Japan, a macrolide antibiotic, tylosin, is used as the approved prophylactic for AFB. Although tylosin-resistant P. larvae has yet to be found in Japan, it may emerge in the future through the acquisition of macrolide resistance genes from other bacteria, and bacteria latent in brood foods, such as honey, may serve as a source of resistance genes. In this study, to investigate macrolide resistance genes in honey, we attempted to isolate tylosin-resistant bacteria from 53 Japanese honey samples and obtained 209 isolates from 48 samples in the presence of 1 μg/ml of tylosin. All isolates were Gram-positive spore-forming bacteria mainly belonging to genera Bacillus and Paenibacillus, and 94.3% exhibited lower susceptibility to tylosin than Japanese P. larvae isolates. Genome analysis of 50 representative isolates revealed the presence of putative macrolide resistance genes in the isolates, and some of them were located on mobile genetic elements (MGEs). Among the genes on MGEs, ermC on the putative mobilizable plasmid pJ18TS1mac of Oceanobacillus strain J18TS1 conferred tylosin and lincomycin resistance to P. larvae after introducing the cloned gene using the expression vector. Moreover, pJ18TS1mac was retained in the P. larvae population for a long period even under non-selective conditions. This suggests that bacteria in honey is a source of genes for conferring tylosin resistance to P. larvae; therefore, monitoring of bacteria in honey may be helpful to predict the emergence of tylosin-resistant P. larvae and prevent the selection of resistant strains.

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“…Most studies investigated bacterial infections, with almost half of all articles focusing on Paenibacillus larvae , the causative agent of American foulbrood (n=9) (2,6,7,13–15,17,18,20). Melissococcus plutonius , the causative agent of European foulbrood, was also investigated in two articles (15,20).…”

Section: Resultsmentioning

confidence: 99%

Section: Susceptibility To Pestsmentioning

confidence: 99%

One Health, One Hive: A scoping review of honey bees, climate change, pollutants, and antimicrobial resistance

Jongh

Harper

Yamamoto

et al. 2020

Preprint

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Anthropogenic climate change and increasing antimicrobial resistance (AMR) together threaten the last 50 years of public health gains. Honey bees are a model One Health organism to investigate interactions between climate change and AMR. The objective of this scoping review was to examine the range, extent, and nature of published literature on the relationship between AMR and honey bees in the context of climate change and environmental pollutants. The review followed systematic search methods and reporting guidelines. A protocol was developed a priori in consultation with a research librarian. Resulting Boolean search strings were used to search Embase® via Ovid®, MEDLINE®, Scopus®, AGRICOLA™ and Web of Science™ databases. Two independent reviewers conducted two-stage screening on retrieved articles. To be included, the article had to examine honey bees, AMR, and either climate change or environmental pollution. Data, in accordance with Joanna Briggs Institute guidelines, were extracted from relevant articles and descriptively synthesized in tables, figures, and narrative form. A total of 21 articles met the inclusion criteria, with almost half of all articles being published in the last five years (n=10). These articles predominantly investigated hive immunocompetence and multi-drug resistance transporter downregulation (n=10), susceptibility to pests (n=15), especially American foul brood (n=9), and hive product augmentation (n=3). This review identified key themes and gaps in the literature, including the need for future interdisciplinary research to explore the link between AMR and environmental change evidence streams in honey bees. We identified three potential linkages between pollutive and climatic factors and risk of AMR. These interconnections reaffirm the necessity of a One Health framework to tackle global threats and investigate complex issues that extend beyond honey bee research into the public health sector. It is integral that we view these “wicked” problems through an interdisciplinary lens to explore long-term strategies for change.

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Determination of tylosins A, B, C and D in bee larvae by liquid chromatography coupled to ion trap-tandem mass spectrometry

Cited by 12 publications

References 34 publications

A rapid and sensitive LC-MS/MS method for the determination of multi-class residues of antibiotics in chicken liver

A rapid and sensitive LC-MS/MS method for the determination of multi-class residues of antibiotics in chicken liver

Antimicrobial Resistance Genes in Bacteria Isolated From Japanese Honey, and Their Potential for Conferring Macrolide and Lincosamide Resistance in the American Foulbrood Pathogen Paenibacillus larvae

One Health, One Hive: A scoping review of honey bees, climate change, pollutants, and antimicrobial resistance

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