Determination of tropane alkaloids atropine and scopolamine by liquid chromatography–mass spectrometry in plant organs of Datura species

Jakabová, Silvia; Vincze, Lajos; Farkas, Ágnes; Kilár, Ferenc; Boros, B; Felinger, Attila

doi:10.1016/j.chroma.2012.02.036

Cited by 116 publications

(76 citation statements)

References 50 publications

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“…The contrast between differentials and gradients of selection for the studied alkaloids indicates that, while direct selection reduces atropine concentration, indirect selection reduces scopolamine. Since atropine is the precursor of scopolamine [51] and their concentrations are positively correlated (as detected in this study; see also Shonle & Bergelson, [16]), direct selection acting on atropine is likely conditioning the adaptive value of scopolamine as well. Nevertheless, in one of the studied populations (Santo Domingo), consumed by the generalist grasshopper S. purpurascens , selection favored an increase in scopolamine and a reduction in atropine concentrations.…”

Section: Discussionsupporting

confidence: 63%

Selection Mosaic Exerted by Specialist and Generalist Herbivores on Chemical and Physical Defense of Datura stramonium

et al. 2014

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Selection exerted by herbivores is a major force driving the evolution of plant defensive characters such as leaf trichomes or secondary metabolites. However, plant defense expression is highly variable among populations and identifying the sources of this variation remains a major challenge. Plant populations are often distributed across broad geographic ranges and are exposed to different herbivore communities, ranging from generalists (that feed on diverse plant species) to specialists (that feed on a restricted group of plants). We studied eight populations of the plant Datura stramonium usually eaten by specialist or generalist herbivores, in order to examine whether the pattern of phenotypic selection on secondary compounds (atropine and scopolamine) and a physical defense (trichome density) can explain geographic variation in these traits. Following co-evolutionary theory, we evaluated whether a more derived alkaloid (scopolamine) confers higher fitness benefits than its precursor (atropine), and whether this effect differs between specialist and generalist herbivores. Our results showed consistent directional selection in almost all populations and herbivores to reduce the concentration of atropine. The most derived alkaloid (scopolamine) was favored in only one of the populations, which is dominated by a generalist herbivore. In general, the patterns of selection support the existence of a selection mosaic and accounts for the positive correlation observed between atropine concentration and plant damage by herbivores recorded in previous studies.

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Section: Discussionsupporting

confidence: 63%

Selection Mosaic Exerted by Specialist and Generalist Herbivores on Chemical and Physical Defense of Datura stramonium

et al. 2014

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“…It is widely used as sedative in clinical practice including preanesthetic medication for general anesthesia, and also for manic psychosis, motion sickness, parkinsonism and organophosphorus pesticide poisoning [2], [3]. Due to its activity in exciting the respiratory center and sedative effect on the cerebral cortex, scopolamine is used to rescue respiratory failure caused by extremely heavy epidemic encephalitis, accompanied by severe frequent tics in such condition [4], [5].…”

Section: Introductionmentioning

confidence: 99%

“…Scopolamine occurres in all plant organs and was traditionally extracted from flowers of Datura species. It was recently reported that the maximum concentrations were found in the stems and leaves of juvenile plants [3], [7]. But the concentration is still quite low and its supply cannot meet the market demand.…”

Section: Introductionmentioning

confidence: 99%

Complete Chloroplast Genome Sequence of Poisonous and Medicinal Plant Datura stramonium: Organizations and Implications for Genetic Engineering

Yang

Dang

et al. 2014

PLoS ONE

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Datura stramonium is a widely used poisonous plant with great medicinal and economic value. Its chloroplast (cp) genome is 155,871 bp in length with a typical quadripartite structure of the large (LSC, 86,302 bp) and small (SSC, 18,367 bp) single-copy regions, separated by a pair of inverted repeats (IRs, 25,601 bp). The genome contains 113 unique genes, including 80 protein-coding genes, 29 tRNAs and four rRNAs. A total of 11 forward, 9 palindromic and 13 tandem repeats were detected in the D. stramonium cp genome. Most simple sequence repeats (SSR) are AT-rich and are less abundant in coding regions than in non-coding regions. Both SSRs and GC content were unevenly distributed in the entire cp genome. All preferred synonymous codons were found to use A/T ending codons. The difference in GC contents of entire genomes and of the three-codon positions suggests that the D. stramonium cp genome might possess different genomic organization, in part due to different mutational pressures. The five most divergent coding regions and four non-coding regions (trnH-psbA, rps4-trnS, ndhD-ccsA, and ndhI-ndhG) were identified using whole plastome alignment, which can be used to develop molecular markers for phylogenetics and barcoding studies within the Solanaceae. Phylogenetic analysis based on 68 protein-coding genes supported Datura as a sister to Solanum. This study provides valuable information for phylogenetic and cp genetic engineering studies of this poisonous and medicinal plant.

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“…Particularly the large-flowered species like D. innoxia and D. metel are popular ornamentals, being potential sources of intoxication if any plant part is consumed either intentionally or accidentally (Hanna et al 1992;Birmes et al 2002;Gupta et al 2003;Steenkamp et al 2004;Diker et al 2007;Wiebe et al 2008;Krenzelok 2010). Alkaloid content and composition largely varies depending on the Datura species and variety, the plant part and the season (Miraldi et al 2001;Berkov et al 2006;Doncheva et al 2006;Iranbakhsh et al 2006;Jakabová et al 2012). We demonstrated that atropine and scopolamine are present not only in various plant parts, but also in the nectar produced by the flowers (Boros et al 2010).…”

Section: Plants Containing Alkaloidsmentioning

confidence: 50%

Medicinal and Aromatic Plants of the World

Máthé¹

2015

Medicinal and Aromatic Plants of the World

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Determination of tropane alkaloids atropine and scopolamine by liquid chromatography–mass spectrometry in plant organs of Datura species

Cited by 116 publications

References 50 publications

Selection Mosaic Exerted by Specialist and Generalist Herbivores on Chemical and Physical Defense of Datura stramonium

Selection Mosaic Exerted by Specialist and Generalist Herbivores on Chemical and Physical Defense of Datura stramonium

Complete Chloroplast Genome Sequence of Poisonous and Medicinal Plant Datura stramonium: Organizations and Implications for Genetic Engineering

Medicinal and Aromatic Plants of the World

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