Determination of trimethoprim and sulfamethoxazole (co-trimoxazole) in body fluids of man by means of high-performance liquid chromatography

Vree, T. B.; Hekster, Y.A.; Baars, A. M.; Damsma, J. E.; Kleijn, Eppo van der

doi:10.1016/s0378-4347(00)81294-3

Cited by 90 publications

(24 citation statements)

References 19 publications

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“…Vree et al [6] described a method for the assay of co-trimoxazole in serum and urine using HPLC. A variable wave length detector is used for optimal detection of TMP.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Conventional Methods and High-Performance Liquid Chromatography for the Determination of Trimethoprim, Sulfamethoxazole and Its Metabolite in Serum

Essers¹,

Korte²

1982

Chemotherapy

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A modified high-performance liquid chromatographic (HPLC) method for sensitive and rapid determination of trimethoprim, sulfamethoxazole and its metabolite N₄-acetylsulfamethoxazole has been compared with the bioassay for trimethoprim and a colorimetric procedure for sulfonamides. The sensitivity of the (HPLC) method has been increased by ultrafiltration of the sample. Thus, the sample dilution was markedly reduced compared to the values obtained with precipitation procedures. The recovery was 102.7 ± 6.1% for trimethoprim, 93 ± 5.4% for sulfamethoxazole and 90.2 ± 7.9% for N₄-acetylsulfamethoxazole. The between-day reproducibility was 5% (n = 5). The coefficients of correlation for HPLC and reference methods were 0.993 (bioassay) and 0.995 (colorimetic assay).

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“…Vree et al [6] described a method for the assay of co-trimoxazole in serum and urine using HPLC. A variable wave length detector is used for optimal detection of TMP.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Conventional Methods and High-Performance Liquid Chromatography for the Determination of Trimethoprim, Sulfamethoxazole and Its Metabolite in Serum

Essers¹,

Korte²

1982

Chemotherapy

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“…All samples were stored at -20°C until assay ed. Serum and sinus secretion concentrations of TMP, SD and 4N-acetyl metabolite of SD were mea sured with the specific high pressure liquid chroma tography method of Vreeet al [ 10], Arithmetic means ±SE were calculated but also the individual values are given in the 'Results'.…”

Section: Methodsmentioning

confidence: 99%

Penetration of Trimethoprim and Sulfadiazine into Sinus Secretion in Acute Maxillary Sinusitis

Mattila¹,

Männistö²,

Luodeslampi³

1983

Chemotherapy

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The concentrations of trimethoprim (TMP), sulfadiazine (SD) and acetyl-SD in serum and maxillary sinus secretions of 10 patients with acute maxillary sinusitis were measured at the steady-state of continuous b.i.d. treatment with 160 mg of TMP + 500 mg of SD. The samples were collected 2–4 h after the preceding dose of TMP/SD. The mean TMP concentrations were 2.97 ± 0.35 µg/ml (mean + SE) and 3.85 ± 1.12 µg/ml in serum and sinus secretion, respectively. The average sinus secretion/serum ratio was 1.33. The mean SD levels in serum and sinus secretion were 41.9 ± 4.5 and 6.64 ± 1.40 µg/ml, respectively, and the average sinus secretion/serum ratio was 0.20. Most patients (7/10) had detectable amounts of acetyl-SD in sinus secretions (mean 1.92 ± 0.87 µg/ml). The corresponding concentrations in serum were 4.56 ± 0.47 µg/ml.

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“…The concentration in serum and tissue fluid of trimethoprim and the parent (active) sulfonamide compounds was determined by using liquid chromatography (15 been performed in humans (1). However, the skin blister technique does not require surgical intervention and is well tolerated.…”

Section: Methodsmentioning

confidence: 99%

Sulfonamide and trimethoprim concentrations in human serum and skin blister fluid

Bruun¹,

Ostby²,

Bredesen³

et al. 1981

Antimicrob Agents Chemother

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Various sulfonamides and trimethoprim were given orally twice a day to healthy volunteers. The drug concentrations in serum and tissue fluid from skin blisters were determined concomitantly. Maximal serum concentrations were obtained after 1 to 3 h. Absorption of sulfacarbamide and sulfadimidine was more rapid than for sulfadiazine, sulfamethoxazole, and trimethoprim. The penetration to blister fluid was delayed and mimal concentrations were usually reached after 4 to 8 h. The highest penetration to blister fluid was found for sulfacarbamide, sulfadiazine, and trimethoprim. During maintenance therapy sulfadiazine and trimethoprim gave blister fluid concentrations usually above 50% of the serum level. However, on the basis of dosage the highest sulfonamide concentration both in serum and blister fluid was obtained with sulfamethoxazole.

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Determination of trimethoprim and sulfamethoxazole (co-trimoxazole) in body fluids of man by means of high-performance liquid chromatography

Cited by 90 publications

References 19 publications

Comparison of the Conventional Methods and High-Performance Liquid Chromatography for the Determination of Trimethoprim, Sulfamethoxazole and Its Metabolite in Serum

Comparison of the Conventional Methods and High-Performance Liquid Chromatography for the Determination of Trimethoprim, Sulfamethoxazole and Its Metabolite in Serum

Penetration of Trimethoprim and Sulfadiazine into Sinus Secretion in Acute Maxillary Sinusitis

Sulfonamide and trimethoprim concentrations in human serum and skin blister fluid

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