Determination of tricyclazole and alcohol metabolite in rice grain and straw

Abstract

An analytical method for determining residues of the fungicide tricyclazole (5-methyl-l,2,4-triazolo-[3,4-b]benzothiazole) and the major metabolite (l,2,4-triazolo[3,4-6]benzothiazole-5-methanol) in rough rice, grain components, and straw has been developed with flame photometric-gas chromatographic detection. Rice plants were foliar sprayed with tricyclazole at booting growth stage with a single 0.5 lb/A application or dual applications of 0.25 lb/A at booting and early heading growth stages. Maximum tricyclazole residues found in rough rice and straw were 3.9 and 3.5 ppm, respectively. Maximum metabolite residues found in rough rice and straw were 1.1 and 10.2 ppm, respectively. The limit of detection for tricyclazole or the alcohol metabolite in rough rice was 0.02 ppm based on a 25.0-g sample. Recoveries at the 0.10 ppm fortification level were approximately 77% for tricyclazole and 57% for the metabolite. The alcohol metabolite was derivatized and chromatographed as the trimethylsilyl (Me3Si) ether.

5-Sulfo anthranilic acid dithiocarbamate functionalized silver nanoparticles as a colorimetric probe for the simple and selective detection of tricyclazole fungicide in rice samples

“…As a result, several analytical techniques such as high performance liquid chromatography (HPLC), [5][6][7][8] gas chromatography (GC), [9][10][11] gas chromatography-mass spectrometry (GC-MS), 12,13 liquid chromatography-mass spectrometry (LC-MS) 14,15 and surface enhanced Raman spectroscopy (SERS) 16 have been used for the detection of tricyclazole in rice samples. These methods provide a high sensitivity, but they are expensive and required tedious sample preparations and a long analysis time.…”

Extraction of Tricyclazole from Soil and Sediment with Subcritical Water

“…Two soil extraction methods that achieved quantitative extraction on freshly spiked samples have been developed (Yunxiang et al, 1990;Tsukioka, 1988), but these methods were not validated on aged samples and also required numerous sample cleanup steps before chromatographic analysis. Methods used to extract tricyclazole from other solid matrixes such as rice grain and straw include refluxing in 4 N H 2 SO 4 (Koons et al, 1985) or organic solvent extraction (Tsumura et al, 1996). These methods require a substantial amount of sample cleanup to remove interfering co-extractants, and recoveries typically are not quantitative.…”