Determination of Trichlorfon and Dichlorvos Residues in Shrimp Using Gas Chromatography with Nitrogen−Phosphorus Detection

Abstract

A method capable of quantifying trichlorfon and dichlorvos in shrimp at concentrations of 20−80 ng/g has been developed. Ground shrimp is homogenized in ethyl acetate and centrifuged. The supernatant is dried completely on a rotatory evaporator. The extract is dissolved in petroleum ether, concentrated on a solid phase extraction column, and analyzed by gas chromatography using a cool on-column inlet and a nitrogen−phosphorus detector. The analytes are separated from matrix components using a thermal gradient on a (cyanopropyl)phenyl-methylpolysiloxane column. The method was validated with control shrimp fortified at 20, 40, and 80 ng/g trichlorfon and dichlorvos. The average recoveries and intralaboratory coefficients of variations were 50−83% and 15−21% respectively. Keywords: Trichlorfon; dichlorvos; shrimp; gas chromatography; nitrogen−phosphorus detector

Determination of Trichlorfon Pesticide Residues in Milk via Gas Chromatography with μ-Electron Capture Detection and GC-MS

“…Due to the intrinsic ultraviolet-detection (UV) spectral action of trichlorfon, the sensitivity for determination trichlorfon using spectrophotometry is low (Talebpour et al ., 2006 ; Zhu et al ., 2008) . Gas chromatographic techniques for the detection and determination of trichlorfon residue in animal tissues, plants, and water systems have been reported (Akhtar 1982 ; Ngoh and Cullison, 1996 ; Brito et al ., 2002 ; Natalia et al ., 2006) . The main purpose of this study was for determining residues of trichlorfon pesticides in milk using gas chromatography with μ-electron capture detection and GCMS.…”

“…(2007) . Trichlorfon is thermally labile and decomposes when analyzed by GC using a heated injector.Quantitation of trichlorfon has been achieved by monitoring the thermal breakdown products such as dichlorvos,dichloroacetaldehyde, and dimethyl phosphate (Ngoh and Cullison, 1996) .…”

“…Trichlorfon residues in fruit and vegetables have been determined until now by gas chromatography with flame photometric detection (FPD) and electron capture detection (ECD). Recently, the use of GC coupled to mass spectrometry (MS) has been considered a suitable approach to improve selectivity and sensitivity (Grimalt et al ., 2006) ; it is thermally labile and decomposes when analyzed by GC using a heated injector (Ngoh and Cullison, 1996) . Analysis of trichlorfon residues has been reported using gas chromatography coupled with FID (Talebpour et al ., 2006) ; FPD (Iwata et al ., 1979 ; Sheets et al ., 1982 ; Maitlen and Halfhill, 1985) ; NPD (Schultz et al ., 1971 ; Ngoh and Cullison, 1996) and mass spectrometry (Aroa et al ., 2002 ; Brito et al ., 2002 ; Talebpour et al ., 2006) , and by high performance liquid chromatography (HPLC) (Arao et al ., 2002 ; Talebpour et al ., 2006 ; Zhu et al ., 2008) .…”

Dissipation kinetics, safety evaluation, and preharvest interval assessment of trichlorfon application on rice

“…However, trichlorfon is still extensively used in agricultural practice on a global scale, and its product volume is even increasing in the developing countries. There have been studies regarding trichlorfon residues on some animals and cabbage (Ngoh and Cullison 1996;Blasco et al 2002;Brito et al 2002;Grimalt et al 2006; Barkat et al 2009;Hem et al 2010;Li et al 2011;Meng et al 2012;Xin et al 2013;Zhao et al 2014;Xue et al 2015).…”

Chiral separation and enantioselective degradation of trichlorfon enantiomers in mariculture pond water

“…While there have been some valuable studies [14][15][16][17][18][19] on the analysis of TF in food, soil, and seawater, to the best of our knowledge, no work has been done on evaluating the enantioselective behavior of TF enantiomers in the environment. In addition, in the former studies, TF enantiomers were regarded as the same material, which causes the analysis results to be unreliable.…”