Determination of trace amounts of albumin in human bronchoalveolar lavage fluids by fluorometry with chromazurol S

Determination of Albumin in Bronchoalveolar Lavage Fluid by Flow-Injection Fluorometry Using Chromazurol S

“…According to CAS manual method procedure, 15) measurement wavelengths (Ex., 500 nm; Em., 620 nm) of fluorescence were determined for measurement. The effects of reagent concentration, mixing volume ratio of reagent solutions, flow rates of carrier solution and reagent mixture, reaction coil length, and operation temperature on signal intensity were examined.…”

“…15) Figure 7 shows the albumin concentrations obtained by the two methods. A good linear correlation was obtained (rϭ0.986).…”

“…Our previous study showed that albumin levels in BALF samples increased in patients with the active stage of sarcoidosis. 15) The clinical stage of patients with sarcoidosis was not investigated in this study.…”

“…13,14) We modified CAS method to determine trace amounts of albumin in BALF. 15) Flow injection analysis is now established as one of the most promising analytical methodologies for automation of routine. Flow injection analyses for protein using dye binding spectrometry and fluorometry were reported.…”