Determination of toxicity of neonicotinoids on the genome level using chemogenomics in yeast

Ušaj, Mojca Mattiazzi; Kaferle, Petra; Toplak, Alenka; Trebše, Polonca; Petrovič, Uroš

doi:10.1016/j.chemosphere.2013.10.063

Cited by 8 publications

(2 citation statements)

References 33 publications

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“…Proteomics is combined with vitality and viability testing as well as the analysis of mitochondrial and total cell lipids. To our knowledge, there was only one previous research using yeast as a model organism and complete formulation of insecticide, Confidor with active substance: Imidacloprid and its additives: DMSO and NMP . Therefore, the results of our research may contribute to better understanding of molecular response to Confidor toxicity and finding the potential indicators of damage in complex organisms.…”

Section: Resultsmentioning

confidence: 88%

“…Two of these additives were investigated—dimethyl sulfoxide (DMSO) and N ‐methyl‐2‐pyrrolidone (NMP) and it was shown that they have in formulations more pronounced (negative) effects than the active components alone. These additives affect negatively the spermatogenesis in animals . NMP is a developmental toxicant which causes malformations, incomplete ossification of skull, and decreased fetal body weights in rats …”

Section: Introductionmentioning

confidence: 99%

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Combining proteomics and lipid analysis to unravel Confidor stress response in Saccharomyces cerevisiae

Justinić

Katić

Uršičić

et al. 2019

Environmental Toxicology

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The yeast Saccharomyces cerevisiae is a useful model for studying the influence of different stress factors on eukaryotic cells. In this work we used the pesticide imidacloprid, in the Confidor formulation, as the stress factor and analyzed its influence on the metabolic activity, proteome and lipid content and composition of Saccharomyces cerevisiae yeast. During the cultivation of yeast, the lowest recommended application dose of Confidor (0.025%, v/v) was added to the growth media and its influence on the mitochondria, cytosol with microsomes, and the whole yeast cells was monitored. The results show that under the stress provoked by the toxic effects of Confidor, yeast cells density significantly decreased and the percentage of metabolically disturbed cells significantly increased comparing with untreated control.Also, there was a downregulation of majority of glycolytic, gluconeogenesis, and TCA cycle enzymes (Fba1, Adh1, Hxk2, Tal1, Tdh1,Tdh3, Eno1) thus providing enough acetyl-CoA for the lipid restructuring and accumulation mechanism since we have found the changes in the cell and mitochondrial lipid content and FA composition.This data suggest that lipids could be the molecules that orchestrate the answer of the cells in the stress response to the Confidor treatment.

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Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Combining proteomics and lipid analysis to unravel Confidor stress response in Saccharomyces cerevisiae

Justinić

Katić

Uršičić

et al. 2019

Environmental Toxicology

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An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 1: new molecules, metabolism, fate, and transport

Giorio

Safer

Sánchez‐Bayo

et al. 2017

Environ Sci Pollut Res

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With the exponential number of published data on neonicotinoids and fipronil during the last decade, an updated review of literature has been conducted in three parts. The present part focuses on gaps of knowledge that have been addressed after publication of the Worldwide Integrated Assessment (WIA) on systemic insecticides in 2015. More specifically, new data on the mode of action and metabolism of neonicotinoids and fipronil, and their toxicity to invertebrates and vertebrates, were obtained. We included the newly detected synergistic effects and/or interactions of these systemic insecticides with other insecticides, fungicides, herbicides, adjuvants, honeybee viruses, and parasites of honeybees. New studies have also investigated the contamination of all environmental compartments (air and dust, soil, water, sediments, and plants) as well as bees and apicultural products, food and beverages, and the exposure of invertebrates and vertebrates to such contaminants. Finally, we review new publications on remediation of neonicotinoids and fipronil, especially in water systems. Conclusions of the previous WIA in 2015 are reinforced; neonicotinoids and fipronil represent a major threat worldwide for biodiversity, ecosystems, and all the services the latter provide.

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Photodegradation behaviour of sethoxydim and its comercial formulation Poast® under environmentally-relevant conditions in aqueous media. Study of photoproducts and their toxicity

et al. 2017

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Determination of toxicity of neonicotinoids on the genome level using chemogenomics in yeast

Cited by 8 publications

References 33 publications

Combining proteomics and lipid analysis to unravel Confidor stress response in Saccharomyces cerevisiae

Combining proteomics and lipid analysis to unravel Confidor stress response in Saccharomyces cerevisiae

An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 1: new molecules, metabolism, fate, and transport

Photodegradation behaviour of sethoxydim and its comercial formulation Poast® under environmentally-relevant conditions in aqueous media. Study of photoproducts and their toxicity

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