Determination of Total Vitamin C in Various Food Matrixes by Liquid Chromatography and Fluorescence Detection

Young, E.R.; Soliman, A.-G. M.

doi:10.1093/jaoac/75.5.887

Cited by 34 publications

(11 citation statements)

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“…The analyses for the nutritional content of M. flabellifolius were based on SANAS (South African National Accreditation System) accredited methods. Vitamin A (Hulshof, 2002), E (Manz & Philipp, 1981), B1 (Sims & Shoemaker, 1993), B2 (Sims & Shoemaker, 1993), and C (Dodson, Young, & Soliman, 1992), where analyzed using previously reported liquid chromatographic techniques.…”

Section: Nutritional Analysismentioning

confidence: 99%

Evaluation of the nutritional composition ofMyrothamnus flabellifolius(Welw.) herbal tea and its protective effect against oxidative hepatic cell injury

et al. 2019

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The nutrient composition of Myrothamnus flabellifolius leaf tea extract (MLTE) and its protective effect against oxidative hepatic cell injury were evaluated. Gallic acid, caffeic acid, ferulic acid, methyl gallate, and epicatechin were identified in MLTE by high‐performance liquid chromatography (HPLC). The tea extract showed an appreciable nutritional content of proximate, sugar, vitamin E, monounsaturated fatty acids, omega 6 and 9 unsaturated fatty acids, as well as considerable amounts of various mineral elements. Nineteen amino acids were found. Moreover, MLTE exhibited potent in vitro antioxidant activities, presumably because of its richness in polyphenols (gallic acid and ferulic acid) and vitamin E. In Chang liver cells, pretreatment with MLTE suppressed oxidative lipid peroxidation (IC50 = 113.11 μg/ml) and GSH depletion (IC50 = 70.49 μg/ml) without causing cytotoxicity. These data support the local consumption of M. flabellifolius herbal tea, which may be used against oxidative stress‐induced diseases while providing the body with necessary nutrients. Practical application Herbal teas are one of the most consumed beverages in the world today, due to their refreshing taste and additional health benefits. Myrothamnus flabellifolius herbal tea is a widely used traditional herbal tea in Southern Africa with potentials for commercialization due to its pleasant flavor. This study, for the first time, reported the nutritional composition of the leaf decoction of M. flabellifolius and its protective effect on hepatic oxidative insults. These results can inform the dietary and nutritional use of the tea for optimum benefits, as well as provide preliminary scientific validation of the use of the herbal tea as an antioxidant beverage with good nutritional value.

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Section: Nutritional Analysismentioning

confidence: 99%

Evaluation of the nutritional composition ofMyrothamnus flabellifolius(Welw.) herbal tea and its protective effect against oxidative hepatic cell injury

et al. 2019

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“…A pure vitamin preparation of known activity is used in a parallel test and serves as the reference standard (Barton-Wright, 1952). Vitamin C content was determined by HPLC and fluorescence detection (Dodson et al, 1992) using the same system described for thiamin and riboflavin. Chromatography was performed with a LiChrosorb RP-18 5 μm (4.6 x 250 mm) column with guard column (Phenomenex, Torrance, CA, USA); mobile phase consisted of 50% methanol; flow rate was 1.0 mL/min; excitation and emission wavelengths were 350 nm and 430 nm, respectively.…”

Section: Chemical Analyses Of Selected Nutrientsmentioning

confidence: 99%

Nutrient content of eight African leafy vegetables and their potential contribution to dietary reference intakes

Jaarsveld

Faber

Heerden

et al. 2014

Journal of Food Composition and Analysis

138

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Nutrient content and potential contribution of one average portion towards nutritional requirements (Recommended Dietary Allowance; RDA) of eight African leafy vegetables (ALVs) was determined. Compared to dark-green leafy vegetables (DGLVs) as sub-group, calcium and magnesium content were similar or considerably higher, vitamin C content was considerably lower, while pigweed had higher potassium content and spider flower similar folate content. All ALVs, except Chinese cabbage, had higher iron content. Black nightshade, pigweed, cowpea and spider flower leaves had higher β-carotene content than DGLVs. For children, pigweed and cowpea leaves emerged as good sources of vitamin A (>75% RDA), followed by spider flower, black nightshade, tsamma melon, Jew's mallow and pumpkin leaves (50 -75% RDA). For iron, pumpkin leaves provided 50 -75% RDA. Black nightshade, tsamma melon, pigweed and cowpea leaves contributed 25 -50% RDA, with Jew's mallow, spider flower and Chinese cabbage providing < 25% RDA. The ALVs were not a good source of zinc. Most ALVs were nutritionally similar to DGLVs. For most nutrients Chinese cabbage had considerably lower values than the other ALVs. Most of the ALVs can considerably contribute to requirements of vitamin A and, to a lesser extent, iron, both critical nutrients for developing countries.

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“…Vitamins B1, B2, and B3 were determined by HPLC using the method described by Wimalasiri and Wills [ 17 ]. Vitamin C was estimated using acetic acid and metaphosphoric acid, followed by determination with HPLC using fluorescence [ 18 ]. Alkaline saponification of the sample involved elimination of fats, liberation of natural retinol in the cells, and hydrolysis of added Vitamin A. Vitamin A was determined by HPLC and detected using UV and fluorescence [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

Oxya hyla hyla(Orthoptera: Acrididae) as an Alternative Protein Source for Japanese Quail

Das

Mandal

2014

International Scholarly Research Notices

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Nutrient composition of the grasshoppers Oxya hyla hyla showed that they are a rich nutrient source containing 687.7 g protein/kg of dry body weight. Their antinutrient values fell within nutritionally acceptable values of the poultry bird Coturnix japonica japonica (Japanese quail). The most required essential amino acids and fatty acids were also present in sufficient amount. For feeding trial nine diets were formulated on an equal crude protein (230 g/kg) basis with grasshopper meal, fish meal, and soybean meal. Three sets of diets with grasshopper meal were prepared with 50 g/kg, 100 g/kg, and 150 g/kg grasshopper of total feed. Similarly, other diet sets were prepared with fish meal and also with soybean meal. Results were compared with another group of Japanese quails fed on a reference diet that was considered as control. Two experiments were conducted with a total number of 600, seven-day-old, Japanese quails. In experiment 1 for determination of growth performance, quails were randomly distributed into ten groups of males and ten groups of females containing 30 birds each. In experiment 2 for determination of laying performance, identical ten groups were prepared in ten repetitions (2 females and 1 male in each group) from the six-week-old birds of experiment 1. Birds of diet set GM2 have gained the highest body weight (male 4.04 g/bird/day; female 5.01 g/bird/day) followed by birds of FM3 diet set (male 3.72 g/bird/day; female 4.40 g/bird/day), whereas birds of reference diet have gained 3.05 g/bird/day for male and 3.23 g/bird/day for female. Feed conversion ratio (FCR) of birds fed with GM2 was the lowest (male 3.33; female 2.97) whereas FCR of R group was higher (male 4.37; female 4.65) than grasshopper meal and fish meal based diets. Hen day production percentage was higher (72.2) in GM2 group, followed by FM3 (63.5) group. R group had lower 1st egg weight (9.0 g), weight gain (8.2 g), percentage of hen day production (41.8%), higher feed intake (33.6 g/day/bird), and age at 1st laid egg than the grasshopper meal and fish meal based diets. So growth and laying performance of the birds were significantly better in grasshopper meal and fish meal added diet fed sets than the reference diet fed group; among all the dietary groups 100 g/kg grasshopper meal added diet mostly gave significantly better results followed by 150 g/kg fish meal added diets. It was ascertained that the O. hyla hyla meal had pronounced positive response on the birds. So, the quails could be easily fed 100 g/kg grasshopper meal added diet as it was the most suitable alternative feedstuff compared to the conventional protein source based diets.

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Determination of Total Vitamin C in Various Food Matrixes by Liquid Chromatography and Fluorescence Detection

Cited by 34 publications

References 0 publications

Evaluation of the nutritional composition ofMyrothamnus flabellifolius(Welw.) herbal tea and its protective effect against oxidative hepatic cell injury

Evaluation of the nutritional composition ofMyrothamnus flabellifolius(Welw.) herbal tea and its protective effect against oxidative hepatic cell injury

Nutrient content of eight African leafy vegetables and their potential contribution to dietary reference intakes

Oxya hyla hyla(Orthoptera: Acrididae) as an Alternative Protein Source for Japanese Quail

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