Determination of Total Protein, Superoxide Dismutase, Catalase Activity and Lipid Peroxidation in Soil Macro-fauna (Earthworm) from Onitsha Municipal Open Waste Dump

Ifemeje, Jonathan C.; Udedi, Stanley Chidi; Okechukwu, A. U.; Nwaka, Andrew C.; Lukong, Colin B.; Anene, I. N.; Egbuna, Chukwuebuka; Ezeude, I. C.

doi:10.9734/jsrr/2015/12552

Cited by 10 publications

(6 citation statements)

References 23 publications

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“…For the successful elimination of oxidative radicals from tissues, the equilibrium between these enzymes is essential (Ifemeje et al 2015). Therefore, because of the aggregation of superoxide radicals and hydrogen peroxide, a reduction in the activity of these enzymes can lead to a variety of damaging impacts.…”

Section: Discussionmentioning

confidence: 99%

“…Lipid peroxidation resulting from the lipid reaction of free radicals is considered an essential aspect of cell damage triggered by a free radical attack (Liu et al 2010). Increased lipid peroxidation has long been known to induce functional degradation of different tissues (Ifemeje et al 2015). Therefore, tissue destruction that leads to complications can be directly due to increased oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

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Mitigating Potentials of Antioxidant-Rich Plants in Earthworms Exposed to Glyphosate

Ayanda

Ajayi

2021

Preprint

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Glyphosate is a non-selective herbicide that has adverse effects on non-target organisms. This present study investigated the toxic effects of glyphosate on earthworms and the potential of antioxidant-rich plants, Ocimum gratissimum and Telfairia occidentalis remediate these effects. Earthworms (Eisenia fetida) were placed into four groups and treated with concentrations of 1, 2 and 3% glyphosate in soil. The last group was a control group. The worms were collected on the 3rd, 7th and 14th, days post-exposure. During these intervals, the weight of the worms and activities of antioxidant enzyme - superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA) were measured to determine the level of antioxidant responses. Furthermore, the percentage of DNA fragmentation was measured to assess the level of DNA damage. Compared with the control group, earthworms exposed to glyphosate and fed with Ocimum gratissimum and Telfairia occidentalis showed varying responses, with increased activities of CAT, SOD, GSH and reduced levels of MDA. Also, decreased fragmented DNA was observed in earthworm groups fed with Ocimum gratissimum and Telfairia occidentalis in comparison with the group treated exclusively with the herbicide. These results suggest that toxicity from glyphosate exposure significantly reduced oxidative damage, lipid peroxidation and DNA damage in Eisenia fetida by the antioxidant-rich plants. It is conceivable that soil organisms could suffer a significant mortality when exposed to high concentrations of glyphosate. The cultivation of these plants should be encouraged while caution should be exercised in the use of the herbicides.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mitigating Potentials of Antioxidant-Rich Plants in Earthworms Exposed to Glyphosate

Ayanda

Ajayi

2021

Preprint

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“…Biochemical Studies: Activities of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) levels and protein concentration were determined according to the methods employed by Ifemeje et al, (2015).…”

Section: Analyses Of Soil Propertiesmentioning

confidence: 99%

Assessment of the environmental impacts of solous municipal solid waste landfills using soil properties and earthworms (<i>Eudrilus eugeniae</i>)

Amadi¹,

Anikwe²,

Obanya³

et al. 2019

Journal of Applied Sciences and Environmental Management

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This study assessed the effects of Solous Municipal Solid Waste Landfills on soil properties, heavy metal contamination and macro-invertebrates (earthworms). Soil and earthworm samples were collected from Solous municipal solid waste landfills with reference samples obtained from the Botanical Garden, University of Lagos and taken to the laboratory for analyses using standard methods. Results showed that pH and organic matter values of soil samples from the landfills ranged from 8.51±0.88 to 9.85±0.02 and 5.79±0.53 % to 7.11±1.19 % respectively, and they were statistically (P < .05) higher than the values of the reference samples. Most of the assessed heavy metals in soil and earthworms from the landfills were significantly (P < .05) higher when compared to the reference samples. The antioxidant enzymes and lipoperoxidation product levels were significantly (P < .05) higher in earthworms from most of the landfills in relation to the reference samples. The mean total protein concentrations in annelids from the landfills were lower than the mean concentration in the reference samples, however, only those from two of the landfills (Solous B and C) were significant (P < .05). Histological studies showed that the clitella of earthworms from the landfills had abnormalities such as disruption of body wall muscle layers, cellular degeneration, oedematous longitudinal muscle, and dark brown pigments. Solous Landfills had an obvious effect on the assessed abiotic and biotic parameters, and could be a source of heavy metal pollution in nearby vicinities especially their groundwater sources and surface water.

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“…The metabolism results to biologic effects which may include pharmacologic response, toxicity, immunological reactions, and cancer. In the human body, some substances such as free radicals in the form of reactive oxygen species (ROS) or reactive nitrogen species (RNS) are continuously produced as byproducts of normal metabolism [4,5] [6]. This increase can be aggravated with the intake of substances capable of inducing oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Effects of Four Different Food Additives on the Oxidative Stress Markers of Wistar Albino Rats

et al. 2019

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This work investigated the effects of food additives on the oxidative stress markers and liver marker enzymes. Food additives are products added to the basic food stuff with an aim of improving its flavor, aspect, texture, color, taste, and value. Thirty (30) Wistar rats were divided into five groups of six rats each. Group A received a daily dose of distillated water and normal rat pellet, Group B, C, D and E received 20 mg/kg body of Aspartame, Sodium Benzoate, Sodium nitrite and sodium sulfite respectively. The duration for exposure of these animals to food additives was 30 days and the groups were observed daily for general conditions. At the end of feeding the animals, blood samples were collected and analyzed. The result of the analysis showed that ALT significantly (p<0.05) increased with sodium nitrite and sodium sulfite while ALP increased significantly (p<0.05) with sodium sulphite. The oxidative stress biomarker, catalase showed a significant increase with sodium benzoate and sodium sulphite. The results revealed that the food additives are injurious to the animals. The toxicity of the food additives studied at 20 mg/kg are in the order of Sodium nitrite > Sodium benzoate > Sodium sulphite > Aspartame.

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Determination of Total Protein, Superoxide Dismutase, Catalase Activity and Lipid Peroxidation in Soil Macro-fauna (Earthworm) from Onitsha Municipal Open Waste Dump

Cited by 10 publications

References 23 publications

Mitigating Potentials of Antioxidant-Rich Plants in Earthworms Exposed to Glyphosate

Mitigating Potentials of Antioxidant-Rich Plants in Earthworms Exposed to Glyphosate

Assessment of the environmental impacts of solous municipal solid waste landfills using soil properties and earthworms (<i>Eudrilus eugeniae</i>)

Effects of Four Different Food Additives on the Oxidative Stress Markers of Wistar Albino Rats

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