Determination of total phenolic content in Prunella L. by horseradish peroxidase immobilized onto chitosan beads

Aybastıer, Önder; Şahin, Saliha; Işık, Esra; Demir, Cevdet

doi:10.1039/c1ay05218g

Cited by 19 publications

(13 citation statements)

References 38 publications

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“…A central composite design (CCD) is suitable for response surface methods because the CCD optimization not only rapidly screens a wide range of conditions but also indicates the role of each factor (Aybastier et al . ). A CCD was used to describe the individual and cumulative effects the parameters had on the response.…”

Section: Introductionmentioning

confidence: 97%

“…Response surface methods have been used widely to produce and optimize different industrially important biotechnological and biochemical products (Bezerra et al 2008;Aybastıer and Demir 2010). A central composite design (CCD) is suitable for response surface methods because the CCD optimization not only rapidly screens a wide range of conditions but also indicates the role of each factor (Aybastier et al 2011). A CCD was used to describe the individual and cumulative effects the parameters had on the response.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Ultrasonic-Assisted Extraction of Quercetin and Cyanidin from P yracantha Coccinea and Their Scavenging Effect on Free Radicals

Şahin

Aybastıer

Demir

2016

Journal of Food Biochemistry

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The response surface method was used to optimize the quercetin and cyanidin content experimental parameters for the ultrasonic-assisted extraction of phenolics from Pyracantha coccinea. The optimal conditions were an extraction time of 97 and 93 min, ethanol concentration of 71 and 79% (v/v), HCl concentration of 1.55 and 1.52 mol/L, liquid-solid ratio of 12.2 and 12.6 mL/g for determining the quercetin and cyanidin contents, respectively. The predicted quercetin and cyanidin contents were 2.30 and 4.01 mg/g, respectively. The experimental values agreed with those predicted at the 95% confidence level, which indicates the response surface method was suitable for optimizing the ultrasound-assisted extraction of phenolics from P. coccinea. The scavenging effects of cyanidin and quercetin in P. coccinea extract, which extracted at optimum conditions, on free radicals were investigated using the Fenton oxidation. It was also demonstrated that cyanidin had more effective free radical scavenging activity than quercetin. PRACTICAL APPLICATIONSThe phenolic compounds differ in polarity, acidity, number of both hydroxyl groups and aromatic rings, concentration and matrix complexity, specific extractions techniques must be designed and optimized for each phenolic compound. Response surface methods have been used widely to produce and optimize different industrially important biotechnological and biochemical products. A central composite design was used to optimize the parameters for P. coccinea extractions based on the quercetin and cyanidin content. The study results indicate the ultrasonic-assisted extraction is an economical and efficient method for extracting quercetin and cyanidin from P. coccinea. The free radical scavenging effects of the two phenolic compounds investigated in this study. They act as free scavengers and inhibit free radical production. This study indicates P. coccinea can be considered a good source of naturally occurring antioxidant compounds.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Optimization of Ultrasonic-Assisted Extraction of Quercetin and Cyanidin from P yracantha Coccinea and Their Scavenging Effect on Free Radicals

Şahin

Aybastıer

Demir

2016

Journal of Food Biochemistry

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“…The use of RSM cannot be overemphasized as it assists in the evaluation of several parameters simultaneously with their interactions by limiting the number of an experiment to be conducted, as well as optimize process parameters and estimation of interactions [9,10]. Central Composite Design (CCD) is amongst one of the several techniques of RSM employed to design experimental procedures which have the advantage of screening a wide range of parameters as well as evaluating single variable/ cumulative effect of the variables to response [11]. It can also determine the number of the experiment to be able to evaluate for optimization of variables and responses [12] and has been found to widely used for the optimization techniques for calcination processes to produce significantly better models compared to other models [13].…”

Section: Introductionmentioning

confidence: 99%

Prediction and Optimization of Sulphur Trioxide Yield from Calcination of Aluminium Sulfate Using Central Composite Design

Olubajo

Makarfi

2019

PoS

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Sulphur trioxides are common toxic gaseous pollutants which can be produced from alternative routes via calcination of aluminum sulfate derived from kaolin clay. Its demand increases geometrically, thus the need to optimize the yield of SO 3 from the calcination of alum is essential. The rate of alum decomposition was monitored by the formation of SO 3 via thermogravimetric analysis and X-ray fluorescence analysis. This study aimed to evaluate the effect of calcination temperature and curing time on the SO 3 conversion and yields using Face Central Composite Design and optimize the process conditions to evaluate the maximum yield of SO 3 using response surface methodology and its effects and interactions were investigated between 800-900 °C at 60-180 minutes. Results indicated that experimental data satisfied second order polynomial regression model for SO 3 conversion and SO 3 yield from TG analysis while XRF analysis satisfied first order model respectively. An increase in SO 3 conversion and yields was observed as the calcination temperature and time were increased both independently and simultaneously. The calcination temperature was found to have a stronger influence compared to the calcination time. Validation indicated agreement between experimental and predicted values with a regression value of 97.8 %, 97.77 % and 97.67 % for SO 3 conversion, SO 3 yield via TG and XRF analyses respectively. Based on the ANOVA, the SO 3 yield via XRF produced the best model with R 2 pred of 91.98% while SO 3 yield via TG analysis and SO 3 conversion had R 2 pred of 79.99% and 78.01% respectively. Optimization of the production of SO 3 was carried out and the optimal condition for SO 3 conversion, SO 3 yield via TG and XRF analyes were 90.11 %, 91.67 % and 75.81 % respectively at an optimal calcination temperature of 877.43 o C and time of 155.04 minutes respectively.

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“…For immobilization, an efficient selection of the supports and immobilization protocols is important. There are many parameters in the supports, including specific surface area, superficial activation degree, pore diameter, mechanical resistance, and so forth . In addition, the release of the enzyme on the support is necessary to avoid enzyme inactivation, because an enzyme‐support attachment that is too strong could be too bad for the properties of the enzyme .…”

Section: Introductionmentioning

confidence: 99%

A novel strategy to synthesize dual‐responsive polymeric nanocarriers for investigating the activity and stability of immobilized pectinase

Lei

Liu

et al. 2019

Biotech and App Biochem

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A dual-stimuli-responsive support material for pectinase immobilization through ionic bonding was prepared. Specifically, polystyrene-b-polymethylacrylic (PS-b-PMAA), light-and pH-sensitive polystyrene-(5-propargylether-2-nitrobenzyl bromoisobutyrate)-b-poly(diethylamino)ethyl methacrylate-b-poly(polyethylene glycol methacrylate) (PS-ONB-PDEAEMA-b-PPEGMA) were synthesized through atom transfer radical polymerization, click chemistry, and hydrolysis. The two parts could self-assemble into the micelles in an aqueous solution. The micelles shrunk at a higher pH, and their size reduced under UV irradiation. The stimuli-responsive properties of micelles were characterized by dynamic light scattering and transmission electron microscopy. It has been found that this support was able to adsorb 10 U/mL of immobilized pectinase (approximately 223 mg/g) at pH 5.0 and 60 • C for 60 Min. Meanwhile, the highest relative activity of immobilized pectinase was up to approximately 95% at pH 5.0 and 60 • C. The immobilized pectinase retained more than 50% of the initial activity after eight cycles. The relative activity of the pectinase immobilized on the supports without UV irradiation was approximately 3% lower than that after UV irradiation at 60 • C, indicating that tailoring of enzyme activity was achieved by changing environmental conditions. Apparently, the original enzymatic support material had a great application prospect on enzyme immobilization.

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Determination of total phenolic content in Prunella L. by horseradish peroxidase immobilized onto chitosan beads

Cited by 19 publications

References 38 publications

Optimization of Ultrasonic-Assisted Extraction of Quercetin and Cyanidin from P yracantha Coccinea and Their Scavenging Effect on Free Radicals

Optimization of Ultrasonic-Assisted Extraction of Quercetin and Cyanidin from P yracantha Coccinea and Their Scavenging Effect on Free Radicals

Prediction and Optimization of Sulphur Trioxide Yield from Calcination of Aluminium Sulfate Using Central Composite Design

A novel strategy to synthesize dual‐responsive polymeric nanocarriers for investigating the activity and stability of immobilized pectinase

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