Determination of total nitrogen in plant material with Nessler's reagent by continuous-flow analysis

Ginkel, J.H. van; Sinnaeve, J.

doi:10.1039/an9800501199

Cited by 26 publications

(11 citation statements)

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“…In agreement with earlier findings, 12,13 that for detection of the initially formed soluble orange product a wavelength of near 400 nm is recommended, the highest sensitivity was obtained for the pair: 395 nm LED and 405 nm LED, used as a light emitter and a detector, respectively. These two LEDs were integrated in the compact form of a flow cell (70 μL total volume and 10 mm optical pathlength).…”

Section: Methodssupporting

confidence: 76%

“…The same problem has been reported in earlier communications. 12,13 To solve the problem of reaction product accumulation inside the system, the use of poly(vinyl alcohol) 12 or potassium sodium tartrate 13 in a carrier as agents preventing precipitate deposition has been recommended. Unfortunately, these attempts were unsuccessful.…”

Section: Resultsmentioning

confidence: 99%

“…The Nessler's method of ammonia determination was adapted to the flow injection analysis (FIA) format, 12,13 although the problem of deposition of the reaction product inside the FIA manifolds was still troublesome. In this short communication we present a modern, miniaturized and economic flow system based on the current trend of multicommutation/multipumping in analytical spectrometry.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Optoelectronic Detector and Improved Flow Analysis Procedure for Ammonia Determination with Nessler’s Reagent

Kołacińska

Koncki

2014

ANAL. SCI.

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This paper presents a simple, rapid and effective method for ammonia determination in a flow analysis regime using Nessler's reagent. The proposed modification of the common flow procedure results in the total elimination of the problem of precipitate deposition inside the flow manifold. The improved procedure has been adapted to a flow analysis system based on microsolenoid pumps combined with a dedicated optoelectronic detector fabricated specifically for this purpose. This photometric device has been constructed in the form of a compact flow-through cell (70 μL total volume and 1 cm optical pathlength) integrated with 395 nm LED emitter and 405 nm LED-based detector. The presented analytical system is capable of ammonia determination in the submillimolar concentration range with a detection limit below 0.1 mM and high throughput (over 20 injections per hour).

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Section: Methodssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Novel Optoelectronic Detector and Improved Flow Analysis Procedure for Ammonia Determination with Nessler’s Reagent

Kołacińska

Koncki

2014

ANAL. SCI.

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“…Total C in the soil solutions was measured by persulphate-u.v. digestion (Van Ginkel & Sinnaeve, 1980) incorporated in an auto-analyser system (Skalar analytical methods 311-411. Breda.…”

Section: Soil Microbial Biomassmentioning

confidence: 99%

Elevated CO₂ enhances below‐ground C allocation in three perennial grass species at different levels of N availability

Cotrufo

Gorissen²

1997

New Phytologist

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summary Three perennial grass species, Lolium perenne L., Agrottis capillaris L. and Festuca uvina L., were homogeneously labelled in phytotrons with 14CO2 at two CO2 concentrations (350 and 700μl l−1). Plants were grown under two nitrogen regimes: one with a minor addition of 8 kg N ha−1, the other with an addition of 278 kg N ha−1. Carbon allocation over the different compartments of the plant/soil systems was measured: shoots, roots, rhizosphere soil (soil solution, microbial biomass and soil residue), and bulk soil. Elevated CO., increased total net 14C recovery in all species by 14%, and significantly enhanced the below‐ground 14C allocation by 26%, this enhancement was 24%, 39% and 21 % for root, rhizosphere soil and bulk soil, respectively. Within the rhizosphere soil, the 14C amounts in the soil solution (+ 69%) and soil residue (+ 49%,) increased significantly. Total microbial biomass‐C in the rhizosphere soil was also increased (15 %) by the elevated CO2 treatment, but only in proportion to the increased root mass. No interactions were observed between the elevated CO2, and N treatments. The N treatment increased total net 14C recovery by more than 300% and 14C was preferentially allocated to the shoots, leading to a significant increase in shoot‐to‐root ratio. However, N fertilization also increased (+111%) the absolute amount of 14C in soil. The three species behaved differently, but no interactions were observed between CO2 treatment and plant species. These results show that elevated CO2 induces an increased C input into soil for all three grass species at both N levels. However, the highest absolute amounts were found in the soils of the fastest growing species and at the highest N level.

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“…For determination of N, separate samples of unhulled grains were digested on a hot plate with sulfuric acid (H 2 SO 4 ) and hydrogen peroxide (16). In wet-digested samples, a colour was developed with Nessler's reagent and absorbance was measured on a spectrophotometer (UV-1602, BMS, Quebec, Canada) 425 nm (17). A factor of 5.7 was used to convert total N measurements to concentration of raw protein in grains.…”

Section: Methodsmentioning

confidence: 99%

Combined zinc and nitrogen applications at panicle initiation for zinc biofortification in rice

et al. 2019

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Background and purpose: Increasing zinc (Zn) concentration in rice grains can help improve Zn nutrition of people. The combinations of Zn and nitrogen (N) applications at panicle initiation were investigated for Zn biofortification in rice. Materials and methods: Rice (cv. Super Basmati) seedling were grown in pots having a calcareous soil. All combinations of four Zn (control, Soil 6 mg Zn kg-1 , foliar 2 × 0.2% Zn and soil + foliar Zn) and three N (control, soil 20 mg N kg-1 and foliar 0.5% N) levels were imposed at panicle initiation. At maturity, grains analysed for Zn and proteins. Results: Grain protein concentration was significantly increased with foliar Zn treatments, and with soil and foliar N. Maximum grain Zn concertation (30 mg kg-1) was achieved with application of soil Zn + foliar Zn + foliar N. At each N level, Zn application by either method significantly increased grain Zn concentration over control. This increase in grain Zn concertation at N levels was 36 to 54% with soil Zn + foliar Zn, 27 to 45% with foliar Zn and 9 to 15% with soil Zn over its control level. Conclusions: Grain Zn concentration was significantly increased with soil N when combined with soil Zn, and with foliar N when combined with foliar Zn treatments. Conclusively, foliar N combined with soil + foliar Zn is the best combination of late Zn and N application for agronomic Zn biofortification in rice.

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Determination of total nitrogen in plant material with Nessler's reagent by continuous-flow analysis

Cited by 26 publications

References 0 publications

A Novel Optoelectronic Detector and Improved Flow Analysis Procedure for Ammonia Determination with Nessler’s Reagent

A Novel Optoelectronic Detector and Improved Flow Analysis Procedure for Ammonia Determination with Nessler’s Reagent

Elevated CO₂ enhances below‐ground C allocation in three perennial grass species at different levels of N availability

Combined zinc and nitrogen applications at panicle initiation for zinc biofortification in rice

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Determination of total nitrogen in plant material with Nessler's reagent by continuous-flow analysis

Cited by 26 publications

References 0 publications

A Novel Optoelectronic Detector and Improved Flow Analysis Procedure for Ammonia Determination with Nessler’s Reagent

A Novel Optoelectronic Detector and Improved Flow Analysis Procedure for Ammonia Determination with Nessler’s Reagent

Elevated CO2 enhances below‐ground C allocation in three perennial grass species at different levels of N availability

Combined zinc and nitrogen applications at panicle initiation for zinc biofortification in rice

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Elevated CO₂ enhances below‐ground C allocation in three perennial grass species at different levels of N availability