Determination of Tocopherols by TLC-Densitometry

Lipophilic Vitamins

“…Mixtures of (3-and y-tocopherol and of /3-and y-tocotrienol could also be separated by GLC (110). TLC was also used for the separation and determination of tocopherol isomers in peanut oil (111), in wheat germ, cottonseed, and soybeans (112), and in other foods (113,114). The derivatives (hydroxy fatty acids), minor lipid compounds, e.g., tocopherol, and aromatic compounds, e.g., menthol, can be purified by TLC and separated and determined by HPLC (115).…”

“…Nonspecific visualization procedures for tocopherols and tocotrienols are based on spraying with sulfuric acid, molybdophosphoric acid ( (1)(2)(3)(4), or bathophenanthroline (112,123). They appear violet, and detection is more sensitive (0.02 /ug) on layers that contain 0.02% Na-fluorescein.…”