Determination of tocopherols and tocotrienols in vegetable oils and fats by high performance liquid chromatography: results of a collaborative study and the standardised method

Pocklington, W. D.; Dieffenbacher, A.

doi:10.1351/pac198860060877

Cited by 80 publications

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“…Fatty acids were quantified by adding methyl heneicosoanate as an internal standard prior to hydrolysis of tissues or lipid extracts. The vitamin E content of the feeds was determined by the method of Pocklington and Dieffenbacher (1988). Vitamin E was extracted from plasma by the method of Burton et al (1985).…”

Section: Methodsmentioning

confidence: 99%

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Kasapidou

Enser

Wood

et al. 2009

Animal

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In order to determine the effect of dietary vitamin E level and basal diet on vitamin E status, performance and tissue fatty acid content, five groups of eight Suffolk 3 Charollais wether lambs with an initial live weight of 28.4 (s.d. 1.6) kg were allocated to one of five concentrate-based diets supplemented with all-rac-a-tocopheryl acetate to contain 30 mg (C-30), 60 mg (C-60), 120 mg (C-120), 250 mg (C-250) or 500 mg (C-500) a-tocopheryl acetate/kg dry matter (DM), for 63 days. Two additional groups of eight lambs entered the study at 31.2 (s.d. 3.3) kg and were fed grass silage and 400 g/day concentrate for 56 days, with the whole diet providing the equivalent of 60 mg (S-60) or 500 mg (S-500) a-tocopheryl acetate/kg DM. Lambs were weighed and blood samples obtained by venipuncture weekly. Dietary vitamin E level did not affect performance ( P . 0.05), but lambs fed grass silage grew more slowly ( P , 0.001) and had a higher ( P , 0.001) feed conversion ratio (kg feed/kg gain) than those fed concentrates. At day 0 plasma a-tocopherol concentrations were 0.8 mg/ml and did not differ between treatments ( P . 0.05). Plasma a-tocopherol concentrations then decreased in all lambs except for those fed S-500, which increased, and at slaughter were (mg/ml) 0.07, 0.23, 0.39, 0.76 and 1.57 in C-30, C-60, C-120, C-250 and C-500 and 1.18 and 1.93 in S-60 and S-500, respectively. At slaughter, muscle and liver a-tocopherol concentrations were in the deficiency range for lambs fed C-30, C-60 or C-120, whereas plasma creatine kinase and tissue polyunsaturated fatty acids were unaffected by dietary vitamin E level, but creatine kinase levels were higher ( P , 0.05) and glutathione peroxidise levels lower ( P , 0.001) in lambs fed grass silage than concentrates alone. Muscle and liver a-tocopherol concentrations were 1.8-and 4.1-fold higher in lambs fed S-60 than C-60, but there was less of a difference between lambs fed S-500 or C-500 with muscle and liver differences of 0.4-and 0.7-fold, respectively. Tissue n-3 polyunsaturated fatty acid concentrations were higher ( P , 0.05) and n-6 fatty acids lower in lambs receiving the grass silage compared to concentrate-based diets, but were not affected by dietary vitamin E level. It is concluded that lower plasma and tissue levels of a-tocopherol are present in lambs supplemented with all-rac-a-tocopheryl acetate on a concentrate compared to a mixed diet of silage and concentrates, and that normal growth can be achieved at tissue levels previously considered to represent deficiency.Keywords: a-tocopherol, concentrates, fatty acids, grass silage, lamb growth ImplicationsVitamin E is necessary to protect tissues from oxidative damage. When lambs were fed a concentrate or grass silage-based diet supplemented with increasing amounts of vitamin E, substantially lower plasma and tissue vitamin E levels were observed in lambs receiving the concentratebased diets. Animal performance was, however, unaffected by dietary vitamin E level. Tissue fatty acid composition was also unaffected by...

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Section: Methodsmentioning

confidence: 99%

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Kasapidou

Enser

Wood

et al. 2009

Animal

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“…Feed samples were bulked over 4-week periods through-out the study for analysis of chemical composition, using the method described by Dewhurst, et al (1999), or for fatty acid composition as described by (Scollan, et al, 2001). Vitamin E analysis of feed was performed using the method described by Pocklington and Dieffenbacher (1988). The chemical composition of experimental feeds is shown in Table 2.…”

Section: Animals and Dietsmentioning

confidence: 99%

Addition of an extract of lucerne (Medicago sativa L.) to cattle diets – Effects on fatty acid profile, meat quality and eating quality of the M. longissimus muscle

et al. 2017

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. Addition of an extract of lucerne (Medicago sativa L.) to cattle diets -Effects on fatty acid profile, meat quality and eating quality of the M. longissimus muscle. Meat Science, 130,[69][70][71][72][73][74][75][76][77][78][79][80] University of Bristol -Explore Bristol Research General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractThere is considerable interest in enhancing beneficial fatty acids, particularly 18:3n-3, conjugated linoleic acid and long chain n-3 fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in beef to provide benefits to human health. Here, the potential to enhance these fatty acids by feeding a n-3 polyunsaturated fatty acid (PUFA)-rich plant extract (PX) from lucerne is presented. Cattle (n=8/diet) were assigned to the following finishing diets: straw and concentrate (S-CC); straw and concentrate containing 25% PX and 2000 IU vitamin E/kg PX-concentrate (S-PXC); grass silage (GS); GS plus 75 g PX/kg dry matter intake (DMI) (GS-LPX) or GS plus 150 g PX/kg DMI (GS-HPX). Addition of PX to concentrates or GS increased the n-3 fatty acid content of loin muscle and had favourable effects on the n-6:n-3 PUFA ratio (P < 0.05), without detrimental effects on loin muscle sensory characteristics.

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“…All participants in the collaborative study, coordinated by Pocklington and Dieffenbacher [10], used NP-HPLC columns. The reported range was very broad, which was explained by the different mobile phases, flow rates, and detectors used by the different participants.…”

Section: Evaluation Of the Hplc Methods Of Determination Of Tocopherolmentioning

confidence: 99%

Analysis of tocopherols by gas-liquid and high-performance liquid chromatography: a comparative study

Greyt¹,

Petrauskaitè

Kellens³

et al. 1998

Fett/Lipid

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Analyse von Tocopherolen mittels Gas-Flüssigkeits-und Hochleistungsflüssigchromatographie: eine vergleichende Untersuchung. Ausgewählte Normalphasen-HPLC-und gaschromatographische Methoden wurden für die Analyse von Tocopherolen in pflanzlichen Ölen untersucht. Wiederholbarkeit, Genauigkeit und/oder Wiederfindung wurden für beide Methoden bestimmt und mit Daten der offiziellen Methodik verglichen. Die größte Genauigkeit wurde mittels der Normalphasen-HPLC-Technik, basierend auf der AOCS-Methode Ce 8-89, erzielt, was wahrscheinlich durch die kurze und einfache Probenaufbereitung erklärt werden kann. Es wurden Tocopherolgehalte von sechzehn voll raffinierten Pflanzenölen mit sowohl GLC als auch HPLC bestimmt. Die Regressionsanalyse zeigte, daß Tocopherolgehalte, die mittels GLC ermittelt worden sind, um 6,1% höher waren als die vergleichbaren HPLC-Daten. Obwohl die ermittelte Wiederfindungsrate der GLC-Methode gut ist, scheint es, daß bei der Probenaufbereitung zusätzlich eine Auftrennung mittels Dünnschichtchromatographie eingeführt werden sollte, um eine Überlappung der Peaks zwischen Tocopherolen und einigen störenden Substanzen der unverseifbaren Fraktion zu vermeiden.a Department of Food Technology and Nutrition, University of Ghent, Gent, Belgium. b De Smet Engineering N. V., Edegem, Belgium.vents and reagents used were analytical or HPLC grade and purchased from Acros Organics (Geel, Belgium). Gas liquid chromatographySample preparation: The selected GLC procedure was based on the AOCS official method Ce 3-74 [6]. 5-7 g of oil were saponified in the presence of 0.15 % butylhydroxytoluene as antioxidant [5]. Tocopherols and sterols were not separated by thin-layer chromatography prior to GLC analysis. Derivatization was accomplished by the addition of 1 ml acetic acid anhydride, keeping the mixture 10 min at 140 °C. After cooling, 1 µl was injected in the gas chromatograph.Gas-chromatographic conditions: Analyses were carried out on a Carlo Erba 4160 chromatograph equipped with a flame-ionization detector (FID) and a split injector (split ratio 1:40) (Carlo Erba, Milano, Italy). A fused silica capillary column coated with a nonpolar stationary phase (CP TM Sil 5CB, 25 m × 0.25 mm internal diameter; 0.25 µm film thickness, Chrompack, The Netherlands) was selected. The oven temperature was kept constant at 290 °C. Injector and detector were maintained at 340 °C. All tocopherol homologues eluted within 15 min. The total analysis time was set at 30 min to assure the elution of all sterols.Identification of the individual tocopherols was realized with pure standards as reference compounds.Quantification: Correction factors were determined for all tocopherols by analyzing different mixtures of the tocopherol standards and an internal standard (3-octadecyloxy-1,2-propanediol). Quantitative analyses were performed with a Shimadzu RC3A integrator. High-performance liquid chromatographyPrinciple: The selected NP-HPLC procedure was based on the previously mentioned IUPAC method 2.432 [5] and the AOCS official method Ce ...

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Determination of tocopherols and tocotrienols in vegetable oils and fats by high performance liquid chromatography: results of a collaborative study and the standardised method

Abstract: Abstract

Cited by 80 publications

References 0 publications

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Addition of an extract of lucerne (Medicago sativa L.) to cattle diets – Effects on fatty acid profile, meat quality and eating quality of the M. longissimus muscle

Analysis of tocopherols by gas-liquid and high-performance liquid chromatography: a comparative study

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