In order to measure the optical parameters of biological tissue accurately and non-invasive, the measurement system based on optical coherence tomography was analyzed and designed from two aspects of hardware and software. The refractive index was calibrated by standard refractive index solutions, experiments were preformed to obtain refractive index of tissue samples and scattering coefficient of IntralipidTMsolution with different concentrations. Results show that the experimental values of refractive index agree with the standard values roughly and scattering coefficient linear relate with concentration in IntralipidTMsolution. The measurement system designed in our experiment is accurate and reliable, simple and feasible.