Determination of Tilmicosin Residues In Chicken, Cattle, Swine, and Sheep Tissues by Liquid Chromatography

Stobba-Wiley, Carolyn M; Chang, Jen P.; Elsbury, David T; Moran, John W; Turner, James M; Readnour, Robin S.

doi:10.1093/jaoac/83.4.837

Cited by 19 publications

(8 citation statements)

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“…In this study, tilmicosin could not be detected at the 9 th day after the last dose except in liver with a concentration of 0.28±0.05 µg/g which is approximately the quarter of the mentioned level. Our results were comparable with Stobba-Wiley et al [19] who modified a validated method for quantitation of tilmicosin residues in swine, cattle, and sheep edible tissues, also chicken fat, skin, and muscles over a concentration range of 0.025-20 µg/g. For chicken kidneys and liver, the method was developed over a range of 0.060-20 µg/g, compared with our results; the lower concentrations ranged from 0.07-0.28 µg/g in muscles, kidneys and liver.…”

Section: Discussionsupporting

confidence: 88%

Detection of Tilmicosin Residues by HPLC and its Effect on Cardiac Enzymes and Hematology in Broiler Chickens

Said

El‐Nabtity

Selim³

et al. 2016

Zagazig Veterinary Journal

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The aim of the present study was to detect the tilmicosin residues in muscles, kidneys and liver of broiler chickens and to investigate its impact on both cardiac enzymes and some hematological parameters. Seventy broiler chickens were divided into two groups; the first was left as a negative control. The second group was given tilmicosin orally (30 mg/kg BW) once/day for three successive days. Reversed phase-high performance liquid chromatography (RP-HPLC) with UV detector at 287 nm and solid phase extraction were used for detecting drug residues in tissue samples. Results indicated a widespread distribution of tilmicosin in most tested tissues. All tissue samples were considered tilmicosin free at the 9 th day after the last oral dose except liver. Tilmicosin elicited a significant reduction in red blood cells count, hemoglobin concentration, packed cell volume, lymphocytes and eosinophiles. On the other hand, heterophiles count and the mean corpuscular volume were increased significantly. White blood cells, monocytes, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration showed non-significant changes. Levels of all cardiac enzymes (Aspartate aminotransferase, lactate dehydrogenase, creatine kinase-MB and Troponin I) were high.

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Section: Discussionsupporting

confidence: 88%

Detection of Tilmicosin Residues by HPLC and its Effect on Cardiac Enzymes and Hematology in Broiler Chickens

Said

El‐Nabtity

Selim³

et al. 2016

Zagazig Veterinary Journal

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“…Although, the preparation methods for extraction of tilmicosin from plasma were available in previous studies, the extraction procedures were complex ( Modric et al, 1998 ; Stobba-Wiley et al, 2000 ). To simplify the extraction procedure, we tested diverse chemical materials (acetonitrile, methyl alcohol, dichloromethane and trichloromethane) and found that dichloromethane was the optimum reagent for tilmicosin extraction.…”

Section: Discussionmentioning

confidence: 99%

The Epidemiologic and Pharmacodynamic Cutoff Values of Tilmicosin against Haemophilus parasuis

Zhang

Hao

et al. 2016

Front. Microbiol.

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The aim of this study was to establish antimicrobial susceptibility breakpoints for tilmicosin against Haemophilus parasuis, which is an important pathogen of respiratory tract infections. The minimum inhibitory concentrations (MICs) of 103 H. parasuis isolates were determined by the agar dilution method. The wild type (WT) distribution and epidemiologic cutoff value (ECV) were evaluated by statistical analysis. The new bronchoaveolar lavage was used to establish intrapulmonary pharmacokinetic (PK) model in swine. The pharmacokinetic (PK) parameters of tilmicosin, both in pulmonary epithelial lining fluid (PELF) and in plasma, were determined using high performance liquid chromatography method and WinNonlin software. The pharmacodynamic cutoff (COPD) was calculated using Monte Carlo simulation. Our results showed that 100% of WT isolates were covered when the ECV was set at 16 μg/mL. The tilmicosin had concentration-dependent activity against H. parasuis. The PK data indicated that tilmicosin concentrations in PELF was rapidly increased to high levels at 4 h and kept stable until 48 h after drug administration, while the tilmicosin concentration in plasma reached maximum levels at 4 h and continued to decrease during 4–72 h. Using Monte Carlo simulation, COPD was defined as 1 μg/mL. Conclusively, the ECV and COPD of tilmicosin against H. parasuis were established for the first time based on the MIC distribution and PK-PD analysis in the target tissue, respectively. These values are of great importance for detection of tilmicosin-resistant H. parasuis and for effective treatment of clinical intrapulmonary infection caused by H. parasuis.

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Section: Methodsmentioning

confidence: 99%

“…Concentrations of tilmicosin in serum were measured by high performance liquid chromatography (HPLC) with UV detection. The HPLC method was based on a previously established procedure reported by Stobba-Wiley et al (2000). The HPLC system consisted of a 126 solvent delivery system, 508 autosampler, and 126 diode array detector (Beckman Coulter, Fullerton, CA, USA).…”

Section: Drug Analysis In Serummentioning

confidence: 99%

Pulmonary disposition of tilmicosin in foals and in vitro activity against Rhodococcus equi and other common equine bacterial pathogens

Womble

Giguère

Murthy

et al. 2006

Vet Pharm & Therapeutics

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The objectives of this study were to determine the serum and pulmonary disposition of tilmicosin in foals and to investigate the in vitro activity of the drug against Rhodococcus equi and other common bacterial pathogens of horses. A single dose of a new fatty acid salt formulation of tilmicosin (10 mg/kg of body weight) was administered to seven healthy 5- to 8-week-old foals by the intramuscular route. Concentrations of tilmicosin were measured in serum, lung tissue, pulmonary epithelial lining fluid (PELF), bronchoalveolar lavage (BAL) cells, and blood neutrophils. Mean peak tilmicosin concentrations were significantly different between sampling sites with highest concentrations measured in blood neutrophils (66.01+/-15.97 microg/mL) followed by BAL cells (20.1+/-5.1 microg/mL), PELF (2.91+/-1.15 microg/mL), lung tissue (1.90+/-0.65 microg/mL), and serum (0.19+/-0.09 microg/mL). Harmonic mean terminal half-life in lung tissue (193.3 h) was significantly longer than that of PELF (73.3 h), bronchoalveolar cells (62.2 h), neutrophils (47.9 h), and serum (18.4 h). The MIC90 of 56 R. equi isolates was 32 microg/mL. Tilmicosin was active in vitro against most streptococci, Staphylococcus spp., Actinobacillus spp., and Pasteurella spp. The drug was not active against Enterococcus spp., Pseudomonas spp., and Enterobacteriaceae.

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Determination of Tilmicosin Residues In Chicken, Cattle, Swine, and Sheep Tissues by Liquid Chromatography

Cited by 19 publications

References 0 publications

Detection of Tilmicosin Residues by HPLC and its Effect on Cardiac Enzymes and Hematology in Broiler Chickens

Detection of Tilmicosin Residues by HPLC and its Effect on Cardiac Enzymes and Hematology in Broiler Chickens

The Epidemiologic and Pharmacodynamic Cutoff Values of Tilmicosin against Haemophilus parasuis

Pulmonary disposition of tilmicosin in foals and in vitro activity against Rhodococcus equi and other common equine bacterial pathogens

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