Determination of ticlopidine in human plasma by high-performance liquid chromatography and ultraviolet absorbance detection

Bo, Lorenzo Dal; Verga, F; Marzo, A; Ambrosoli, L.; Poli, Anicleto

doi:10.1016/0378-4347(94)00539-h

Cited by 11 publications

(1 citation statement)

References 9 publications

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“…[9,10] A limited number of analytical techniques have been used for the determination of TP in drugs and biological samples. TP concentrations were determined by liquid chromatography [11][12][13][14][15][16][17][18] and gas chromatography [19][20][21] coupled with different types of detectors in these studies. The proposed method in the European Pharmacopoeia [22] is also a gas chromatographic method with flame-ionization detection.…”

Section: Determination Of Ticlopidine In Pharmaceuticalmentioning

confidence: 99%

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Can

Altıokka

Aboul‐Enein

2008

Journal of Liquid Chromatography & Related Technologies

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A precise and accurate flow injection analysis method for the quantification of ticlopidine in pharmaceuticals is described. An aqueous carrier stream, which was entirely prepared with water was chosen for the flow injection analysis. The method development was achieved by using a reference standard solution of ticlopidine at 3.19 Â 10 À6 M concentration, which was prepared in water. The solution was injected into the instrumental system at a flow rate of 1.0 mL Á min À1 and signals were detected by a UV detector at 214.2 nm. The calibration curves of TP were linear in the concentration range of 1.59 Â 10 À6 À 7.99 Â 10 À5 M. The intra-and inter-assay precision was less than 1.3% relative standard deviation. The method exhibited good linearity with the correlation coefficients close to unity. The limit of detection and limit of quantization concentrations were found to be 8.91 Â 10 À8 and 2.70 Â 10 À7 M, respectively. The effects of the tablet excipients were insignificant at the 95% probability level. The calculated tablet contents were around 99%, which is in agreement with the ranges stated by pharmacopoeias.

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Section: Determination Of Ticlopidine In Pharmaceuticalmentioning

confidence: 99%

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Can

Altıokka

Aboul‐Enein

2008

Journal of Liquid Chromatography & Related Technologies

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Ticlopidine quantification in human plasma by high‐performance liquid chromatography coupled to electrospray tandem mass spectrometry. Application to bioequivalence study

et al. 2004

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A rapid, sensitive and specific method to quantify ticlopidine in human plasma using clopidogrel as the internal standard (IS) is described. The analyte and the IS were extracted from acidified plasma by liquid-liquid extraction using diethyl ether-hexane (80 : 20, v/v). The extracts were analyzed by high-performance liquid chromatography coupled to electrospray tandem mass spectrometry (HPLC/MS/MS). Chromatography was performed isocratically on a Jones Genesis C(8) 4 microm analytical column (150 x 4.1 mm i.d.). The method had a chromatographic run time of 3.0 min and a linear calibration curve over the range 1.0-1000 ng ml(-1) (r(2) > 0.999427). The limit of quantification was 1.0 ng ml(-1). This HPLC/MS/MS procedure was used to assess the bioequivalence of two ticlopidine 250 mg tablet formulations (ticlopidine test formulation from Apotex do Brasil, Brazil, and Ticlid from Sanofi-Synthelabo, standard reference formulation). A single 250 mg dose of each formulation was administered to healthy volunteers. The study was conducted using an open, randomized, two-period crossover design with a 2 week washout interval. Since the 90% confidence interval for C(max) and area under the curve ratios were all inside the 80-125% interval proposed by the US Food and Drug Administration, it was concluded that ticlopidine formulation from Apotex do Brasil is bioequivalent to Ticlid formulation with respect to both the rate and the extent of absorption.

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Preparation of a ticlopidine potentiometric sensor and its application to pharmaceutical and biological analysis

Nezhadali

Esfandiari

2016

Journal of Electroanalytical Chemistry

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Determination of ticlopidine in human plasma by high-performance liquid chromatography and ultraviolet absorbance detection

Cited by 11 publications

References 9 publications

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Ticlopidine quantification in human plasma by high‐performance liquid chromatography coupled to electrospray tandem mass spectrometry. Application to bioequivalence study

Preparation of a ticlopidine potentiometric sensor and its application to pharmaceutical and biological analysis

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