Determination of Thiodiglycol and Its Oxide in Biomedical Samples by Gas Chromatography–Mass Spectrometry

Glutathione conjugates of the mercapturic acid pathway and guanine adduct as biomarkers of exposure to CEES, a sulfur mustard analog

“…Protease was purchased from Qiagen and phosphodiesterase I from Worthington (Lakewood, NJ, USA). Isotopically labelled molecules Cys* ( 13 C3 99%, 15 N 99%), NAC* ( 13 C3 97-99% 15 N 97-99%), GSH* ( 13 C2 98% 15 N 96-99%) and dGuo* ( 15 N5 98%) were purchased from Cambridge Isotope Laboratories (Andover, USA).…”

“…Rapid, simple and quantitative analytical methods were developed for this early SM biomarker present in blood for a rapid diagnostic during the asymptomatic latency period (less than 12h) [13,14]. The directly hydrolyzed metabolite thiodiglycol (TDG) was detected in urine from SM-treated rats for 48h [15]. Under its bound and its oxidized forms, it was present up to 7 day in the blood and urine of the animals.…”

Electrostatic assemblies of molecularly imprinted polymers on the surface of electrospun nanofiber membranes for the point-of-care detection of thiodiglycol, a sulfur mustard poisoning metabolic marker

“…15,16 Usually, TDG is degraded by alkylation reagents and silanization reagents, such as N,O-bis(trimethylsilyl) triuoroacetamide (BSTFA) and N-methyltriuoroacetamide (MTBSTFA), to chemical substances with similar structures that can be separated and quantied by GC/MS or GC/MS/MS. 17 The direct determination of TDG in complex samples by LC-MS or GC-MS usually has certain disadvantages, such as a complex peak shape, weak signal, and high detection limitation. [18][19][20] In addition, most of these methods require expensive instruments, professional operation, and unaffordable special reagents.…”