Determination of Thickness, Dielectric Constant of Thiol Films, and Kinetics of Adsorption Using Surface Plasmon Resonance

Damos, Flávio Santos; Luz, Rita C. S.; Kubota, Lauro T.

doi:10.1021/la0487038

Cited by 116 publications

(135 citation statements)

References 31 publications

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“…According to the literature, the formation kinetics of alkane-thiol self-assembled monolayer were investigated using various physico-chemical techniques including electrochemical [26], AFM [32], surface plasmon resonance (SPR) [33] and ellipsometry [34,35]. Even though there are some contradictions concerning the rate of formation of the layer, most of the studies deal with a two-stage adsorption process in agreement with what is observed herein.…”

Section: Adsorption-induced Cantilever Bendingsupporting

confidence: 58%

“…Even though there are some contradictions concerning the rate of formation of the layer, most of the studies deal with a two-stage adsorption process in agreement with what is observed herein. In principle, the present results may be compared with the two time scales identified by ellipsometric monitoring of adsorption process [35], even though it has been reported that the mechanical effect and the optical thickness of the adsorbed layer growth kinetics are not simply related [36]. For this reason and also because the adsorption process homogeneity along the micro-cantilever is difficult to assess, a deeper description of the kinetics of formation of the monolayer lies beyond the scope of the present paper.…”

Section: Adsorption-induced Cantilever Bendingmentioning

confidence: 93%

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Charge redistribution in electrochemically actuated mechanical sensors

Amiot

Kanoufi

Hild

et al. 2009

Sensors and Actuators A: Physical

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to real-life applications also requires the monitored mechanical effect to be highly specific. Moving towards more specificity in cantilever-based sensing, monitoring the mechanical response of electrochemically actuated microcantilevers is then thought to provide a fast, reliable and complementary experimental information to the longtime cantilever bending measurement for the detection of target molecules. Full-field measurements are therefore used to investigate the way the electro-elastic coupling is altered when a microcantilever undergoes decane-thiol adsorption. The proposed technique reveals that the latter results in a charge density redistribution along the cantilever in addition to the local surface passivation. Focusing on the cantilever tip displacement under electrochemical actuation, this redistribution partially compensates the electro-elastic coupling alteration due to the surface passivation, therefore possibly yielding an ambiguous detection result. This effect should be taken into account for the optimal design of specific electrochemically actuated mechanical sensors.

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Section: Adsorption-induced Cantilever Bendingsupporting

confidence: 58%

Section: Adsorption-induced Cantilever Bendingmentioning

confidence: 93%

Charge redistribution in electrochemically actuated mechanical sensors

Amiot

Kanoufi

Hild

et al. 2009

Sensors and Actuators A: Physical

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“…57 Thus, these results confirm that interaction between HRP and ds-DNA layer is thermodynamically favourable. Further investigations of stability and applicability of this system will be performed.…”

supporting

confidence: 68%

Kinetic studies of HRP adsorption on ds-DNA immobilized on gold electrode surface by EIS and SPR

Ferreira

Kubota

2010

J. Braz. Chem. Soc.

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Este trabalho descreve a construção de um filme de multicomponentes via método layer-bylayer (LbL) e a análise cinética da interação da enzima peroxidase de raiz forte (HRP) sobre a camada de ds-DNA de timo de bezerro formada numa superfície de ouro modificada. As técnicas de ressonância de plásmon de superfície (SPR) e a espectroscopia de impedância eletroquímica (EIE) foram usadas para caracterizar a construção do filme sobre a superfície de ouro. A ressonância de plásmon de superfície forneceu informações essenciais para o estudo e caracterização da interação proteína e ácido nucléico. Este método não necessita de marcador e monitora as interações em tempo real. Os estudos cinéticos obtidos por SPR da formação do filme de HRP sobre o ds-DNA apresentaram valores de 24,7 L mol -1 s -1 e 1,2×10 -3 s -1 para k a e k d , respectivamente. A energia livre de Gibbs obtida para o sistema foi de -23,1 kJ mol -1. Os resultados obtidos mostram que a interação das moléculas da enzima sobre o ds-DNA é cinética e termodinamicamente favoráveis e a interação entre as camadas ocorre principalmente pela atração de cargas opostas. This paper describes the construction of a multicomponent film via layer-by-layer (LbL) method and the kinetic analysis of the interaction between horseradish peroxidase (HRP) enzyme on calf thymus double-stranded DNA layer on a modified gold surface. Surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) are used to characterize the successful construction of the film on the gold surface. Surface plasmon resonance provided essential information for the study and characterization of protein and nucleic acid interaction and this method is label-free and monitors the interactions in real time. The kinetic studies determined by SPR of the horseradish peroxidase film formation on ds-DNA layer showed values of 24.7 L mol -1 s -1 and 1.2×10 -3 s -1 for k a and k d , respectively. The Gibbs free energy obtained for the system was -23.1 kJ mol -1. The results obtained show that the interaction of the enzymes molecules on ds-DNA is kinetically and thermodynamically favourable and the interaction among the layers probably occurs mainly by attraction of opposite charges.Keywords: SPR, EIS, kinetic adsorption, DNA, HRP, protein-DNA interaction IntroductionThe interaction of nucleic acids with protein has been a primary area of interest for therapeutic development and in the study of cellular processes.1-3 Development of devices based on this interaction presents application in the investigations of the toxicity pathways of nucleic acid. Recently, electrochemical sensors have been developed for the rapid detection of DNA damage. [4][5][6][7] Such devices are being successfully used in food industry, environmental fields and clinical chemistry. Biosensors for such analysis can be prepared with multicomponent films of polyions and other charged materials and to achieve high specificity, high sensitivity, rapid response and flexibility of use, it is clear that the research continues to focus ...

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“…28 However, one should keep in mind that the Au film in our case is significantly rougher with respect to the evaporatively coated films which have been used in the previous reports. This fact may be at the origin of the discrepancy in the overall adsorption kinetics between previously reported data and our findings.…”

Section: Resultsmentioning

confidence: 85%

Submicrometer Cavity Surface Plasmon Sensors

et al. 2005

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A miniaturized spherical surface plasmon sensor for measuring the binding kinetics of unlabeled molecules is introduced. The sensor has a submicrometer footprint with a sensitivity that rivals that of state-of-the-art commercial planar surface plasmon sensors, which makes it valuable for applications requiring integration of detection of molecular species in microfluidic channels. The basic principle of the sensor is exploiting the wavelength shifts of the cavity resonances of a metal-coated submicrometer sphere embedded in an opaque metal film due to molecular adsorption. The sensor has been found to be exquisitely responsive in air to water and ethanol vapor adsorption on the bare gold sensor surface. When immersed in a liquid, the sensor can detect the adsorption of less than one monolayer of dodecanethiol (∼1.5 nm) on the gold coating of the sphere.

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Determination of Thickness, Dielectric Constant of Thiol Films, and Kinetics of Adsorption Using Surface Plasmon Resonance

Cited by 116 publications

References 31 publications

Charge redistribution in electrochemically actuated mechanical sensors

Charge redistribution in electrochemically actuated mechanical sensors

Kinetic studies of HRP adsorption on ds-DNA immobilized on gold electrode surface by EIS and SPR

Submicrometer Cavity Surface Plasmon Sensors

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