Determination of thiamine, riboflavin and pyridoxine in pharmaceuticals by synchronous fluorescence spectrometry in organized media

García, Luciano; Barrena-Blázquez, Silvestra; Andrés, María Paz San; Vera, S.

doi:10.1016/s0003-2670(01)00836-4

Cited by 59 publications

(27 citation statements)

References 21 publications

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“…Based on literature it was possible to associate some regions of this map to specific fluorophors, namely Phe, Tyr, Trp , pyridoxine, and riboflavin Marose et al, 1998;García et al, 2001). In bioreaction culture bulks, the maximum fluorescence emission for Trp normally reported corresponds to excitation at 290 nm Horvath et al, 1993;Surribas et al, 2006c).…”

Section: Process and 2d Fluorescence Dynamicsmentioning

confidence: 99%

In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures

Teixeira

Portugal

Carinhas

et al. 2008

Biotech & Bioengineering

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The main objective of the present study was to investigate the use of in situ 2D fluorometry for monitoring key bioprocess variables in mammalian cell cultures, namely the concentration of viable cells and the concentration of recombinant proteins. All studies were conducted using a recombinant Baby Hamster Kidney (BHK) cell line expressing a fusion glycoprotein IgG1-IL2 cultured in batch and fed-batch modes. It was observed that the intensity of fluorescence signals in the excitation/emission wavelength range of amino acids, vitamins and NAD(P)H changed along culture time, although the dynamics of single fluorophors could not be correlated with the dynamics of the target state variables. Therefore, multivariate chemometric modeling was adopted as a calibration methodology. 2D fluorometry produced large volumes of redundant spectral data, which were first filtered by principal components analysis (PCA). Then, a partial least squares (PLS) regression was applied to correlate the reduced fluorescence maps with the target state variables. Two validation strategies were used to evaluate the predictive capacity of the developed PLS models. Accurate estimations of viable cells density (r(2) = 0.95; 99.2% of variance captured in the training set; r(2) = 0.91; 97.7% of variance captured in the validation set) and of glycoprotein concentration (r(2) = 0.99 and 99.7% of variance captured in the training set; r(2) = 0.99 and 99.3% of variance captured in the validation set) were obtained over a wide range of reactor operation conditions. The results presented herein confirm that 2D fluorometry constitutes a reliable methodology for on-line monitoring of viable cells and recombinant protein concentrations in mammalian cell cultures.

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Section: Process and 2d Fluorescence Dynamicsmentioning

confidence: 99%

In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures

Teixeira

Portugal

Carinhas

et al. 2008

Biotech & Bioengineering

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“…4,5 The standard 6 and official analytical methods for vitamin analysis 7 are tedious and involve a pre-treatment of the sample through complex chemical, physical, or biological reactions in order to eliminate interference of other constituents in the vitamin, which is typically followed by individual methods for each different vitamin. Many analytical methods, such as those based on UV-Vis spectrophotometry, 8 fluorimetry, 9 chemiluminiscence, 10 capillary electrophoresis, 11 microbiology, 12 and high-performance liquid chromatography [13][14][15][16][17] have been proposed only for the determination of water-soluble or fat-soluble vitamins and are tedious, sometimes nonspecific, and time-consuming. 18 Among them, both normal and reversed-phase HPLC 19 techniques are most widely used and provide rapid, sensitive, and accurate vitamin analysis and are often used with detection modes, including UV spectrometry with a diode array, fluorometry, and electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Küçükkolbaşı¹,

Bilber²,

Ayyıldız³

et al. 2013

Quím. Nova

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Recebido em 14/10/12; aceito em 22/2/13; publicado na web em 24/5/13In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R 2 > 0.999) over the concentration range of 10-500 ng mL −1. The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.

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“…The deficiency of VB 6 has been suggested as the cause of many types of illness and disease [1]. Several analytical methods have been described in literature for determination of VB 6 , including spectrophotometry [2,3], liquid chromatogramphy [4,5] and electrochemistry method [6,7]. R. Jimé-nez-Prieto et al determined VB 6 by spectrophotometric techniques in the presence of other vitamins [8].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Vitamin B6 at Glassy Carbon Electrode Modified with Gold Nanoparticles and Multi-Walled Carbon Nanotubes

Zhang¹,

Wang²

2011

AJAC

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In this work, the gold nanoparticles (Au NPs)/multi-walled carbon nanotubes (MWCNTs) composite film modified glassy carbon electrode (GCE) was fabricated, and scanning electron microscopy (SEM) was used to investigate the assemble process of the composite film. In pH 7.0 PBS, an oxidation peak of the vitamin B 6 (VB 6 ) was only observed at composite film modified electrode. Under the optimized conditions, the current intensity was linear with the concentrations of VB 6 in the range of 1.59 to 102.74 μg·mL -1 with a detection limit of 0.53 μg·mL -1 (S/N = 3). The modified electrode had been applied in pharmaceutical analysis, and obtained good results.

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Determination of thiamine, riboflavin and pyridoxine in pharmaceuticals by synchronous fluorescence spectrometry in organized media

Cited by 59 publications

References 21 publications

In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures

In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Voltammetric Determination of Vitamin B6 at Glassy Carbon Electrode Modified with Gold Nanoparticles and Multi-Walled Carbon Nanotubes

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