Determination of the viscoelastic behavior of sodium hyaluronate in phosphate buffered saline with rheo-mechanical and rheo-optical methods

Meyer, F. A.; Lohmann, Derek; Kulicke, Werner‐Michael

doi:10.1122/1.3122985

Cited by 16 publications

(27 citation statements)

References 51 publications

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“…46 Light scattering experiments indicate an overlap concentration of c* % 0:05 wt: % for this HA molecule in PBS solution, suggesting that our solutions are in the semi-dilute regime. 42,52 All of the test solutions were prepared by weighing the HA and protein powders into a glass container and adding the appropriate volume of PBS solution. In order to avoid mechanical degradation of the HA during dissolution, magnetic stirring was avoided; instead occasional gentle manual agitation was applied to the containing vessel until the solution appeared completely transparent and homogenous.…”

Section: Test Fluids and Their Rheological Characterizationmentioning

confidence: 99%

“…3,38,39 A full characterization and understanding of the rheological properties of HA in solution is therefore vital for the optimization of the fluids used for this form of therapy. While such rheological studies are numerous for simple shearing flows that are relevant to joint flexion, 11,[40][41][42][43] detailed studies of extensional flows of HA solutions that could be relevant to SF function during joint compression remain scarce. [11][12][13]44 Backus et al 13 used an opposed-jets device, in "blowing mode," in order to apply a biaxial extensional flow to solutions of two different high molecular weight HA samples (one linear and one cross-linked) used in viscosupplementation therapy.…”

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confidence: 99%

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Extensional flow of hyaluronic acid solutions in an optimized microfluidic cross-slot device

et al. 2013

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We utilize a recently developed microfluidic device, the Optimized Shape Crossslot Extensional Rheometer (OSCER), to study the elongational flow behavior and rheological properties of hyaluronic acid (HA) solutions representative of the synovial fluid (SF) found in the knee joint. The OSCER geometry is a stagnation point device that imposes a planar extensional flow with a homogenous extension rate over a significant length of the inlet and outlet channel axes. Due to the compressive nature of the flow generated along the inlet channels, and the planar elongational flow along the outlet channels, the flow field in the OSCER device can also be considered as representative of the flow field that arises between compressing articular cartilage layers of the knee joints during running or jumping movements. Full-field birefringence microscopy measurements demonstrate a high degree of localized macromolecular orientation along streamlines passing close to the stagnation point of the OSCER device, while micro-particle image velocimetry is used to quantify the flow kinematics. The stress-optical rule is used to assess the local extensional viscosity in the elongating fluid elements as a function of the measured deformation rate. The large limiting values of the dimensionless Trouton ratio, Tr $ O(50), demonstrate that these fluids are highly extensional-thickening, providing a clear mechanism for the load-dampening properties of SF. The results also indicate the potential for utilizing the OSCER in screening of physiological SF samples, which will lead to improved understanding of, and therapies for, disease progression in arthritis sufferers. V C 2013 AIP Publishing LLC. [http://dx

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Section: Test Fluids and Their Rheological Characterizationmentioning

confidence: 99%

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confidence: 99%

Extensional flow of hyaluronic acid solutions in an optimized microfluidic cross-slot device

et al. 2013

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“…48 In addition to the PEO2 solutions, a series of semi-dilute aqueous solutions of the polysaccharide hyaluronic acid (HA) are utilized. In this case the solvent viscosity is kept 50 and the results are provided in Table I. Note that for a given molecular weight, the extensibility of HA is much lower than that of PEO.…”

Section: A Test Fluidsmentioning

confidence: 99%

Instabilities in stagnation point flows of polymer solutions

Haward

McKinley

2013

Physics of Fluids

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A recently-developed microfluidic device, the optimized shape cross-slot extensional rheometer, or OSCER [S.J. Haward, M.S.N. Oliveira, M.A. Alves and G.H. McKinley, Phys. Rev. Lett. 109, 128301 (2012)], is used to investigate the stability of viscoelastic polymer solutions in an idealized planar stagnation point flow. Aqueous polymer solutions, consisting of poly(ethylene oxide) and of hyaluronic acid with various molecular weights and concentrations, are formulated in order to provide fluids with a wide range of rheological properties. Semi-dilute solutions of high molecular weight polymers provide highly viscoelastic fluids with long relaxation times, which achieve a high Weissenberg number (Wi) at flow rates for which the Reynolds number (Re) remains low; hence the elasticity number El = Wi/Re is high. Lower concentration solutions of moderate molecular weight polymers provide only weakly viscoelastic fluids in which inertia remains important and El is relatively low. Flow birefringence observations are used to visualize the nature of flow instabilities in the fluids as the volume flow rate through the OSCER device is steadily incremented. At low Wi and Re, all of the fluids display a steady, symmetric and uniform 'birefringent strand' of highly oriented polymer molecules aligned along the outflowing symmetry axis of the test geometry, indicating the stability of the flow field under such conditions. In fluids of El > 1, we observe steady elastic flow asymmetries beyond a critical Weissenberg number, Wi crit , that are similar in character to those already reported in standard cross-slot geometries [e.g. P.E. Arratia, C.C. Thomas, J. Diorio and J.P. Gollub, Phys. Rev. Lett. 96, 144502 (2006)]. However, in fluids with El < 1 we observe a sequence of timedependent inertio-elastic instabilities beyond a critical Reynolds number, Re crit , characterized by high frequency spatiotemporal oscillations of the birefringent strand. By plotting the critical limits of stability for the various fluids in the Wi-Re operating space, we are able to construct a stability diagram delineating the distinct steady symmetric, steady asymmetric and inertio-elastic flow regimes in this idealized planar elongational flow device.

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“…This value of intrinsic viscosity gives a viscosity averaged molecular weight of the HA used in this study of 1.2 3 10 6 g mol 21 , agreeing with M w values reported for bacterial HA falling within the range of 7.9 3 10 5 to 1.9 3 10 6 g mol 21 . 34,37 Experimental Conditions for Gel formation Different experimental conditions were tested in order to obtain hyaluronan (HA) gels of high mechanical stability and fast responsivity. As detailed in the experimental section, aqueous HA solutions in the presence of EGDE crosslinker were subjected to gelation in plastic syringes for four days.…”

Section: Fourier Transform-infrared (Ft-ir) Spectrometermentioning

confidence: 99%

“…34 Preparation of HA Gels HA solutions were prepared by weighing out HA using a Shimadzu AUW220D scale and adding it to deionized water (18.2 MX cm at 25 C). The dispersion was gently stirred at 4 C for at least 15 h, to ensure complete dissolution of the polymer.…”

Section: Determination Of Intrinsic Viscositymentioning

confidence: 99%

Preparation and physical properties of hyaluronic acid‐based cryogels

Ström

Larsson

Okay

2015

J of Applied Polymer Sci

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Macroporous hydrogels based on hyaluronan (HA), a natural polysaccharide occurring in extracellular matrix, have attracted interest over many years owing to their numerous applications in the biomedical area. However, HA hydrogels produced so far suffer from low mechanical strength and slow rate of response against external stimuli, which limit their applications. Here, we prepared macroporous HA cryogels of high mechanical stability and fast responsivity from aqueous HA solutions at subzero temperatures using ethylene glycol diglycidyl ether as a crosslinking agent. HA cryogels are squeezable and no crack development was exhibited when compressed up to 80% strain. Depending on the synthesis parameters, the cryogels exhibit an elastic modulus between 0.2 and 2 kPa, and show fast swelling/deswelling behavior. The microstructure of the cryogels consists of large, interconnected pores on the order of 100 mm separated by thick pore walls, as observed by scanning electron microscopy and confocal scanning laser microscopy.

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Determination of the viscoelastic behavior of sodium hyaluronate in phosphate buffered saline with rheo-mechanical and rheo-optical methods

Cited by 16 publications

References 51 publications

Extensional flow of hyaluronic acid solutions in an optimized microfluidic cross-slot device

Extensional flow of hyaluronic acid solutions in an optimized microfluidic cross-slot device

Instabilities in stagnation point flows of polymer solutions

Preparation and physical properties of hyaluronic acid‐based cryogels

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