Determination of the triterpenoid, betulinic acid, in Doliocarpus schottianus by HPLC

Abstract

The isolation, detection and quantification of betulinic acid in Doliocarpus schottianus are described. The isolation from the plant extract was made by column chromatography and centrifugal TLC, and betulinic acid was characterized by spectrometric methods. The detection and quantification were made by HPLC using a C18 column eluted with acetonitrile: water and detected at 210 nm. The results showed that the metabolite accumulates in the bark of the plant, but very small concentrations were also found in the leaves and wood.

Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC

“…This is the major limitation in the analysis of this class of compounds using UV detection. However, there are some reports on triterpenoids and saponins detection at low wavelength range 25,26 in other plants. In some cases derivatization 26 has also been used for the detection at higher wavelengths, but this additional step may results in significant error in the method.…”

Simultaneous determination of betulin and betulinic acid in white birch bark using RP-HPLC

“…Analysis of betulin in Grewia tiliaefolia by HPTLC method has been described [6]. Betulinic acid has been determined by HPLC in Doliocarpus schottianus and by GC-MS after derivatisation in Platanus acerifolia and in Betula pendula [7,8]. The aim of this study was to develop a simple method for the simultaneous determination of betulin and betulinic acid in the white birch bark by HPLC with UV detection.…”

Subcritical water extraction of betulinic acid from birch bark

“…By modifying the method from Oliveira d. et al (2002), our tailored HPLC analysis method was performed. An aliquot (20 L) of each extract was injected into the HPLC column and elution was carried out while acetonitrile: water (with 0.5% acetic acid) = 88: 12, at a flow-rate of 1 mL/min.…”