Determination of the toxicity of several aromatic carbonylic compounds and their reduced derivatives onPhanerochaete chrysosporiumusing aPseudomonas putidatest system

Hage, A.; Wever, Ron; Zennaro, Elisabetta; Heipieper, Hermann J.

doi:10.1002/1097-0290(20010405)73:1<69::aid-bit1037>3.0.co;2-w

Cited by 18 publications

(4 citation statements)

References 31 publications

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“…The presence of an important proportion of acetophenone in the culture media created with soil heated at 300 and 450 °C could be another component limiting microbial proliferation. The toxicity of acetophenone on micro-organisms has been previously studied, reporting fungal and yeast growth inhibition [ 91 , 92 ] and also the existence of different micro-organisms able to degrade it by different metabolic pathways [ 93 ].…”

Section: Resultsmentioning

confidence: 99%

Soil pH and Soluble Organic Matter Shifts Exerted by Heating Affect Microbial Response

Bárcenas-Moreno

Jiménez-Compán

Emeterio

et al. 2022

IJERPH

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Fire-induced alterations to soil pH and organic matter play an important role in the post-fire microbial response. However, the magnitude of which each parameter affects this response is still unclear. The main objective of this work was to determine the magnitude in which soil pH and organic matter fire-induced alterations condition the response of viable and cultivable micro-organisms using laboratory heating, mimicking a range of fire intensities. Four heating treatments were applied to unaltered forest soil: unheated, 300, 450, and 500 °C. In order to isolate the effect of nutrient or pH heating-induced changes, different culture media were prepared using soil:water extracts from the different heated soils, nutrient, and pH amendments. Each medium was inoculated with different dilutions of a microbial suspension from the same original, unaltered soil, and microbial abundance was estimated. Concurrently, freeze-dry aliquots from each soil:water extract were analyzed by pyrolysis-gas chromatography/mass spectrometry. The microbial abundance in media prepared with heated soil was lower than that in media prepared with unheated soil. Nutrient addition and pH compensation appear to promote microbial proliferation in unaltered and low-intensity heated treatments, but not in those heated at the highest temperatures. Soil organic matter characterization showed a reduction in the number of organic compounds in soil-heated treatments and a marked increase in aromatic compounds, which could be related to the observed low microbial proliferation.

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Section: Resultsmentioning

confidence: 99%

Soil pH and Soluble Organic Matter Shifts Exerted by Heating Affect Microbial Response

Bárcenas-Moreno

Jiménez-Compán

Emeterio

et al. 2022

IJERPH

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“…In a study by Heipiepier et al (26), EC 50 s of 3.6 and 3.2 mmol L −1 were reported for aerobic Pseudomonas putida exposed to toluene and styrene, two solvents with log P o/w of 2.48 and 3.0, respectively. Hage et al reported EC 50 s of 8.0, 13.0, 1.5, 3.0, 0.5 and 1.9 mmol L −1 for P. putida growing in the presence of various organic compounds with log P o/w in the range 1.72–3.46 (25). Another aerobe, Acinetobacter calcoaceticus , showed EC 50 s of between 6.0 and 0.11 mmol L −1 when exposed to alkanols of log P o/w of between 1.87 and 3.97, with EC 50 s decreasing with increasing log P o/w (30).…”

Section: Discussionmentioning

confidence: 99%

Tolerance of Anaerobic Bacteria to Chlorinated Solvents

2014

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The aim of this research was to evaluate the effects of four chlorinated aliphatic hydrocarbons (CAHs), perchloroethene (PCE), carbon tetrachloride (CT), chloroform (CF) and 1,2-dichloroethane (1,2-DCA), on the growth of eight anaerobic bacteria: four fermentative species (Escherichia coli, Klebsiella sp., Clostridium sp. and Paenibacillus sp.) and four respiring species (Pseudomonas aeruginosa, Geobacter sulfurreducens, Shewanella oneidensis and Desulfovibrio vulgaris). Effective concentrations of solvents which inhibited growth rates by 50% (EC50) were determined. The octanol-water partition coefficient or log Po/w of a CAH proved a generally satisfactory measure of its toxicity. Most species tolerated approximately 3-fold and 10-fold higher concentrations of the two relatively more polar CAHs CF and 1,2-DCA, respectively, than the two relatively less polar compounds PCE and CT. EC50 values correlated well with growth rates observed in solvent-free cultures, with fast-growing organisms displaying higher tolerance levels. Overall, fermentative bacteria were more tolerant to CAHs than respiring species, with iron- and sulfate-reducing bacteria in particular appearing highly sensitive to CAHs. These data extend the current understanding of the impact of CAHs on a range of anaerobic bacteria, which will benefit the field of bioremediation.

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“…The phenolic compounds by-product stream contains acetophenone. Acetophenone toxicity has been reported at concentrations in the range of 0.4 to 30 mM [36][37][38]. This concentration is roughly 4-fold higher than that of acetophenone concentration present in the bacterial consortium cultures when added with the maximum concentration they can tolerate.…”

Section: Psmentioning

confidence: 99%

Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters

et al. 2021

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Molecular and metabolomic tools were used to design and understand the biodegradation of phenolic compounds in real industrial streams. Bacterial species were isolated from an industrial wastewater treatment plant of a phenol production factory and identified using molecular techniques. Next, the biodegradation potential of the most promising strains was analyzed in the presence of a phenolic industrial by-product containing phenol, alfa-methylstyrene, acetophenone, 2-cumylphenol, and 4-cumylphenol. A bacterial consortium comprising Pseudomonas and Alcaligenes species was assessed for its ability to degrade phenolic compounds from the phenolic industrial stream (PS). The consortium adapted itself to the increasing levels of phenolic compounds, roughly up to 1750 ppm of PS; thus, becoming resistant to them. In addition, the consortium exhibited the ability to grow in the presence of PS in repeated batch mode processes. Results from untargeted metabolomic analysis of the culture medium in the presence of PS suggested that bacteria transformed the toxic phenolic compounds into less harmful molecules as a survival mechanism. Overall, the study demonstrates the usefulness of massive sequencing and metabolomic tools in constructing bacterial consortia that can efficiently biodegrade complex PS. Furthermore, it improves our understanding of their biodegradation capabilities.

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Determination of the toxicity of several aromatic carbonylic compounds and their reduced derivatives onPhanerochaete chrysosporiumusing aPseudomonas putidatest system

Cited by 18 publications

References 31 publications

Soil pH and Soluble Organic Matter Shifts Exerted by Heating Affect Microbial Response

Soil pH and Soluble Organic Matter Shifts Exerted by Heating Affect Microbial Response

Tolerance of Anaerobic Bacteria to Chlorinated Solvents

Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters

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