Determination of the Th1, Th2, Th17, and Treg cytokine profile in patients with chronic Chagas heart disease and systemic arterial hypertension

Bestetti, Reinaldo B.; Dellalibera-Joviliano, Renata; Lopes, Gabriel S; Faria-Jr, Milton; Daniel, Rosemary Aparecida Furlan; Lopes, Kenio C; Batista, Divino R

doi:10.1007/s00380-018-1228-z

Cited by 20 publications

(22 citation statements)

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“…Simultaneous activation of Th1 and Th2 cells was shown in various renal pathologies [69][70][71] and in a number of other diseases [72][73][74]. Moreover, significantly increased IL-9 might suggest ongoing differentiation of Т-cells into a Th9 population [73].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

et al. 2020

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Chronic kidney disease (CKD) is an important public health problem in the world. The aim of our research was to identify novel potential serum biomarkers of renal injury. ELISA assay showed that cytokines and chemokines IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, Eotaxin, FGFb, G-CSF, GM-CSF, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-1bb, RANTES, TNF-α and VEGF were significantly higher (R > 0.6, p value < 0.05) in the serum of patients with CKD compared to healthy subjects, and they were positively correlated with well-established markers (urea and creatinine). The multiple reaction monitoring (MRM) quantification method revealed that levels of HSP90B2, AAT, IGSF22, CUL5, PKCE, APOA4, APOE, APOA1, CCDC171, CCDC43, VIL1, Antigen KI-67, NKRF, APPBP2, CAPRI and most complement system proteins were increased in serum of CKD patients compared to the healthy group. Among complement system proteins, the C8G subunit was significantly decreased three-fold in patients with CKD. However, only AAT and HSP90B2 were positively correlated with well-established markers and, therefore, could be proposed as potential biomarkers for CKD.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

et al. 2020

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“…In addition, most of the infants transferred for other reasons had an extremely low birth weight and congenital malformations. Since the incidence of CHD in these infants is often higher than that in other infants in hospital, 10 timely tracking these infants’ CHD screening results in other hospitals can better reflect the detection rate of CHD screening in newborns.…”

Section: Discussionmentioning

confidence: 99%

A Study on Clinical Screening of Neonatal Congenital Heart Disease in Jinjiang City

Zhang¹,

Bai²

2021

IJGM

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Objective This study explored the feasibility of congenital heart disease (CHD) screening by combining a percutaneous oxygen saturation (POX) test with cardiac auscultation method in neonates. Methods POX tests and cardiac auscultation were used concurrently to screen 8305 neonates born in Jinjiang City Hospital between January 2016 and December 2018 for CHD. The positive screening results (positive POX or positive cardiac auscultation) were confirmed with echocardiography, while any false negative results were identified through follow-up and parent feedback. Sensitivity, specificity, positive/negative predictive values, Youden’s index, and the area under the receiver operator characteristic curve (AUC) of the single use and combined use of the two methods (a POX test and auscultation) were calculated, and the results were compared. Results Among 8305 neonates, 22 cases were positive for POX alone, of which 6 cases were diagnosed by echocardiography; 83 cases were positive for cardiac auscultation alone, of which 47 cases were diagnosed by echocardiography; and 8 cases were positive for both methods, all of which were confirmed by echocardiography. Four more cases were confirmed during follow-up. Sensitivity, specificity, and the positive and negative predictive values of combined screening were 93.85%, 99.37%, 53.98% and 99.95%, respectively, while Youden’s index was 0.93, and the AUC was 0.966. Sixty-five cases of CHD were diagnosed, the total incidence being 7.82%, and a ventricular septal defect was found to be the most common type. Conclusion The combination of POX test and cardiac auscultation as a screening method for neonatal CHD can reduce missed diagnoses and increase the detection rate of CHD in newborn infants.

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“…Several studies showed that IL-17A has an important immunomodulatory role in the chronic phase of the disease 8,14,15,41 . IL-17 expression by Th17 cells and B cells were found in patients with cardiac involvement more frequently, compared to asymptomatic patients, correlating with worse cardiac function 14 ; IL-17 exacerbated produce a proinflammatory environment in Chagas severe heart disease 42,43 .…”

Section: Discussionmentioning

confidence: 99%

“…In response to the infection, IL17-A, induces a rapid proinflammatory cascade of chemokines and cytokines that facilitates the recruitment and activation of neutrophils and monocytes required for the early control of the pathogen by the immune system 12,13 . In the chronic phase of the disease, several studies suggest that the clinical progression of Chagas cardiomyopathy involves the overexpression of IL-17 by Th17 cells and B cells 14,15 . by means of the enzyme-linked immunosorbent assay (ELISA) that uses recombinant antigen and a commercial indirect hemagglutination test.…”

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confidence: 99%

Genetic polymorphisms of IL17A associated with Chagas disease: results from a meta-analysis in Latin American populations

Strauss

Palma-Vega

Casares-Marfil

et al. 2020

Sci Rep

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Genetic factors and the immunologic response have been suggested to determine the susceptibility against the infection and the outcome of chagas disease. in the present study, we analysed three IL17A genetic variants (rs4711998, rs8193036 and rs2275913) regarding the predisposition to Trypanosoma cruzi infection and the development of chronic Chagas cardiomyopathy (CCC) in different Latin American populations. A total of 2,967 individuals from Colombia, Argentina, Bolivia and Brazil, were included in this study. The individuals were classified as seronegative and seropositive for T. cruzi antigens, and this last group were divided into asymptomatic and ccc. for T. cruzi infection susceptibility, the IL17A rs2275913*A showed a significant association in a fixed-effect meta-analysis after a Bonferroni correction (p = 0.016, OR = 1.21, 95%CI = 1.06-1.41). No evidence of association was detected when comparing ccc vs. asymptomatic patients. However, when ccc were compared with seronegative individuals, it showed a nominal association in the meta-analysis (p = 0.040, oR = 1.20, 95%CI = 1.01-1.45). For the IL17A rs4711998 and rs8193036, no association was observed. in conclusion, our results suggest that IL17A rs2275913 plays an important role in the susceptibility to T. cruzi infection and could also be implicated in the development of chronic cardiomyopathy in the studied Latin American population.Chagas disease caused by the protozoan Trypanosoma cruzi is a parasitic infection endemic in Latin American countries, which is nowadays increasingly becoming a global health problem, due to migration to non-endemic areas 1,2 . Around 6 to 7 million people are estimated to be infected worldwide, mostly in Latin America 1,3 . During the acute phase of the disease, the increase of parasitic load induces an inflammatory process for the control of the pathogen 4 . Ten to thirty years after infection, around 30 to 40% of chronically infected patients can develop cardiomyopathy or/and megaviscera. The symptoms include cardiac conduction abnormalities, myocardial contractile dysfunction, arrhythmias, dysphagia, regurgitation, and severe constipation, among others. The cardiac involvement is the most frequent manifestation of the disease 5,6 . The characteristics of this phase vary in different patients and in different regions of the endemic area 7 .After the infection with T. cruzi, interleukin-17A (IL-17A) is produced by T helper 17 (Th17) cells, subset of CD4+ T cells, and innate lymphoid cells 8,9 . More recently, it has been described that B cells are also an important source of IL-17A and IL-17F, produced as well after T. cruzi infection 10,11 . In response to the infection, IL17-A, induces a rapid proinflammatory cascade of chemokines and cytokines that facilitates the recruitment and activation of neutrophils and monocytes required for the early control of the pathogen by the immune system 12,13 . In the chronic phase of the disease, several studies suggest that the clinical progression of Chagas cardiomyopathy involves th...

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Determination of the Th1, Th2, Th17, and Treg cytokine profile in patients with chronic Chagas heart disease and systemic arterial hypertension

Cited by 20 publications

References 50 publications

Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

Proteomic Analysis of Human Serum from Patients with Chronic Kidney Disease

A Study on Clinical Screening of Neonatal Congenital Heart Disease in Jinjiang City

Genetic polymorphisms of IL17A associated with Chagas disease: results from a meta-analysis in Latin American populations

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