Determination of the targeted carbendazim metabolites in zebrafish water tank by liquid chromatography coupled to high-resolution mass spectrometry

Untargeted Metabolomics Reveals Lipid Impairment in the Liver of Adult Zebrafish (Danio rerio) Exposed to Carbendazim

“…The metabolites at m/z 161.0544 must were produced from an O ‐dealkylation reaction, which is an oxidation reaction performed by zebrafish CYP enzymes (Goldstone et al, 2010). In addition, the metabolite at m/z 134.07132 corresponds to the metabolite 2‐AB already reported in our study about the metabolites formed through carbendazim biotransformation by zebrafish in water samples of aquaria containing zebrafish (zebrafish water tank samples; Costa et al, 2022). These metabolites might be used to ensure detectability and increase confidence in monitoring carbendazim exposure.…”

“…Carbendazim and its metabolites were extracted from the water samples using the analytical method developed and validated in the literature (Costa et al, 2022). Briefly, 10-ml aliquots of the samples were submitted to enzymatic hydrolysis.…”

“…Recently, the metabolites formed through carbendazim biotransformation by zebrafish have been identified in water samples from aquaria containing zebrafish (zebrafish water tank samples; Costa et al, 2022). However, apart from targeted metabolites, it is important to evaluate the untargeted metabolites because xenobiotics may also trigger the perturbation of endogenous metabolites, and profiling the dysregulated endogenous metabolome can promote an understanding of biological effects, highlighting biomarkers (Chen et al, 2019).…”

Photoinduced Electron Transfer-Triggered g-C3N4\Rhodamine B Sensing System for the Ratiometric Fluorescence Quantitation of Carbendazim

“…For that, chemical sensors and biosensors play an increasingly important role in environmental monitoring and food quality control. − In this context, optical sensing technologies, with their intrinsic simplicity, high sensitivity, miniaturized devices, and simple, fast, cost-effective, high-throughput, user-friendly field detection, have attracted tremendous attention, especially for monitoring trace pesticides. , Carbendazim (methyl-2-benzimidazole carbamate, Car) as a common pesticide belongs to the benzimidazole family and has been widely used in agriculture for pre- and post-harvest use, primarily in fruit and cereal crops to control several fungal plant diseases such as spot, mildew, mold, rot, and scorch. , However, carbendazim residues can cause a wide range of poisoning symptoms in people and livestock. Even worse, carbendazim has been classified as a potential human carcinogen based on various toxic side effects. − Thus, developing an efficient and reliable optical analytical method is in high demand to measure the number of carbendazim residues.…”